Background: \r\n', u'Rotavirus is the major important cause of infectious infantile gastroenteritis and diarrhea, especially the children aged 6 months to 24 months. Some efforts made to provide safe water and cultivate the hygiene have not reduced the diarrhea caused by rotaviruses. Develope a vaccine agaisnt this virus is of high importance. A question about the production of this kind of vaccine targeted athow to make human rotaviruses strains adapt cultured cells.\r\n', u'Objectives\r\n', u'To determine the multiplicity of infection (MOI) of human rotaviruses strains in vero cell.\r\n', u'Subjects and method: \r\n', u'The multiplicity of infection of Human rotaviruses strains (G1 P8, G1P4, and G4P6) were selected and the CDC- Atlanta US supplied the vero cell.Use vero cells cultivate, temper the vero cell and, applying direct immunofluorescence to determine the multiplicity of G1P8, G1P4, C4P6.\r\n', u'Results:\r\n', u'The MOI of G1P8, G1P4 and C4P6 are 0.02 ffu/cell, 0.15ffu/cell, and 0.35 ffu/cell, respectively.\r\n', u'Conclusion:\r\n', u'Using the vero cell to multiple the rotaviruses is the effective part during the production of vaccines against rotaviruses. The MOI of human rotaviruses strains G1P8, G1P4 and G4P6 are in order:0,02 ffu/cell, 0,15ffu/cell, and 0,35% ffu/cell. \r\n', u'
Rotavirus ; Vero Cells ; Cell Culture Techniques ;

Background: In recent years, the rotavirus diarrhea increased significantly in Vietnam, an estimated 50%-70% of children\u2019s hospitalization for diarrhea in 2005. The virus subtype causing disease mainly in Vietnam today was not only G1, G2, G4, like other countries in the world but also the rare strain was G9. Objectives: to isolate G9 rotavirus strains on MA104 cell culture; to selecting G9 rotavirus which developing well on MA104 cells. Subjectives and Method: an experimental research in the laboratory. 20 stool samples derived from 20 children with acute diarrhea caused by rotavirus G9P8 (using RT-PCR method). The samples were processed according to standards of the Centers for disease control and prevention (CDC), Atlanta, USA. Results: eight out of 20 human rotavirus strains G9P8 were positive on Ma104 cell suspension after 3 consecutive cultures (OD indexes of these sample were over 0.100,sample No 2 had the highest OD (1.347)). Sample No 2 was chosen for the first time cloning (25 clones, 17/25 with OD>0.100). And clone No 16 was selected for the second purifying (25 clones, 24/25 with OD>0.100). Ten out of 25 clones in the second time were adapted on monolayer Ma104 cell culture and only clone No 14 and No 18 with highest OD (2.648 and 2.644, respectively) will be used for next studies. Conclusions: cloning method was a basic method for adapting rotavirus clone on cell cultured. Rate of G9P8 rotavirus strain which isolated on Ma104 cells\ufffd?suspension was relatively high. Among the second time clones, 2/10 samples adapted on Ma104 cells with high OD.
Cell Culture Techniques ; Rotavirus ;

Twenty Rota virus strains were isolated from 20 stool specimens derived from diarrhea children in Khanh Hoa province. After 3 times of passaged culturations on MAI04 cells, there were only 2 human Rota virus strains growing well in the medium of trypsin concentration at 5 mg/ml and 4 human Rotavirus strains multiflying well in the medium of trypsin concentration at 10 mg/ml.
Rotavirus ; Diarrhea, Child ; Child

Rota virus of the type of G4P6, 8 (VNHR 203) is one of endemic strains in Vietnam causing acute diarrhea in under- five -year -old children. In the year 2000 this strain was isolated from fecal sample of a 9 month boy with acute diarrhoae admitted to St Paul hospital. Viruses were carelully selected and underwent 10 consecutive transferred culturing on Rhesus (MA104) monkey kidney cell medium, then 20 times of assimilating on Macacca mulatta monkey primitive kidney cells.They had got high safety and efficacy in the trial on Macacca mulatta aged 12-24 months
Macaca mulatta ; Haplorhini ; Rotavirus ; Diarrhea ; child

41 rotavirus strains that were collected at Hai Phong Children Hospital were determined G, P genotypes by Rt-PCR method. Of these, G9 was the most common (75.62%), followed by G4 (12.19%), G1 (4.87%), and untyped (9.75%). For P genotype, P8 (46.34%), P6 (43.90%) were the most common. The common phenotype of rotavirus strains in Hai Phong Children Hospital were G9P8 (43.90%), G9P6 (21.95%), and G4P6 (12.20%)
Gastroenteritis ; Rotavirus ; child ; diagnosis ; epidemiology

Using rotavirus cultural methods isolated from 2001019203 strain in cellular fluid containing trypsine and rotavirus cloning method in stool samples of diarrhea children at the age of 9 months old, treated in St. Paul's Hospital in 2001. The results: 2001019203 strain was type G4, which was developed in MA 104 cellular fluids. 6/11 clones of the second cloning rotavirus were adaptable and development in cellular fluid MA104 were selected, in which only 3 clones were both adaptable and development as A-1, C-1* and E-3. All 6 adaptable colony were selected into further study
Rotavirus ; Macaca mulatta ; kidney ; cells

10 strains of rotavirus C-1P6 causing acute diarrhoea in under 5- year - old - children were cultured on African Rhesus monkey kidney cells. Only 4 strains were adapted in the mixture of monkey MA104 kid cells. Results showed that in the most favorable medium, not all strains of virus could be adapted and grown properly. Rotaviruses of A group is the main agents causing acute diarrhoea in children in winter
Diarrhea ; Rotavirus ; Kidney ; child ;

Four line of cells Hep2, Vero, MA104 and JVKT are used for quality control and a line of monkey kidney cells used for production of poliomyelitis vaccine. Contaminated mycoplasma was tested by culture method and immu-mark mycotest. Same results were given: 50% of Hep 2 cells and 66.6% of MA104 were contaminated with mycoplasma. There is not any controlled lot of JVKT. Vero and monkey kidney which was contaminated
Poliomyelitis ; Mycoplasma ; Quality Control ; Vaccines

Rotavirus strains isolated from 3 acute diarrhea calves at Ba Vi cattle farm were cultured and firstly cloned in African green monkey kidney (Vero) cells and African Rhesus monkey kidney (MA104) cells. After 3 times of passage, the calf rotavirus strains were secondly cloned and all of them were adapted in primary kidney cells of Macaca mulatta
Rotavirus ; Cell Culture Techniques ; Diarrhea

By RT-PCR biomolecular technique on ARN isolated from 30% fecal mixture of 41 samples of under five years old children with acute diarrhoea due to rotavirus collected at Khanh Hoa Pediatric Hospital, the year 2002, 4 genotypes (G9, G1, G2 and G4), 3 P genotypes (P4, P6, P8) and 2 common phenotypes G1P8 and G2P4 were determined. While 9.75% of G gene and 21,95% of P gene were not determined yet
Diarrhea ; Rotavirus ; Child