41 rotavirus strains that were collected at Hai Phong Children Hospital were determined G, P genotypes by Rt-PCR method. Of these, G9 was the most common (75.62%), followed by G4 (12.19%), G1 (4.87%), and untyped (9.75%). For P genotype, P8 (46.34%), P6 (43.90%) were the most common. The common phenotype of rotavirus strains in Hai Phong Children Hospital were G9P8 (43.90%), G9P6 (21.95%), and G4P6 (12.20%)
Gastroenteritis ; Rotavirus ; child ; diagnosis ; epidemiology

Using rotavirus cultural methods isolated from 2001019203 strain in cellular fluid containing trypsine and rotavirus cloning method in stool samples of diarrhea children at the age of 9 months old, treated in St. Paul's Hospital in 2001. The results: 2001019203 strain was type G4, which was developed in MA 104 cellular fluids. 6/11 clones of the second cloning rotavirus were adaptable and development in cellular fluid MA104 were selected, in which only 3 clones were both adaptable and development as A-1, C-1* and E-3. All 6 adaptable colony were selected into further study
Rotavirus ; Macaca mulatta ; kidney ; cells

10 strains of rotavirus C-1P6 causing acute diarrhoea in under 5- year - old - children were cultured on African Rhesus monkey kidney cells. Only 4 strains were adapted in the mixture of monkey MA104 kid cells. Results showed that in the most favorable medium, not all strains of virus could be adapted and grown properly. Rotaviruses of A group is the main agents causing acute diarrhoea in children in winter
Diarrhea ; Rotavirus ; Kidney ; child ;

Four line of cells Hep2, Vero, MA104 and JVKT are used for quality control and a line of monkey kidney cells used for production of poliomyelitis vaccine. Contaminated mycoplasma was tested by culture method and immu-mark mycotest. Same results were given: 50% of Hep 2 cells and 66.6% of MA104 were contaminated with mycoplasma. There is not any controlled lot of JVKT. Vero and monkey kidney which was contaminated
Poliomyelitis ; Mycoplasma ; Quality Control ; Vaccines

Rotavirus strains isolated from 3 acute diarrhea calves at Ba Vi cattle farm were cultured and firstly cloned in African green monkey kidney (Vero) cells and African Rhesus monkey kidney (MA104) cells. After 3 times of passage, the calf rotavirus strains were secondly cloned and all of them were adapted in primary kidney cells of Macaca mulatta
Rotavirus ; Cell Culture Techniques ; Diarrhea

By RT-PCR biomolecular technique on ARN isolated from 30% fecal mixture of 41 samples of under five years old children with acute diarrhoea due to rotavirus collected at Khanh Hoa Pediatric Hospital, the year 2002, 4 genotypes (G9, G1, G2 and G4), 3 P genotypes (P4, P6, P8) and 2 common phenotypes G1P8 and G2P4 were determined. While 9.75% of G gene and 21,95% of P gene were not determined yet
Diarrhea ; Rotavirus ; Child

2455 fecal samples of acute diarrhoea children collected from National Institute of Pediatries, Hai Phong Pediatric hospital, Khanh Hoa Pediatric hospital, HoChiMinh city Pediatric hospitals No1 and No2 in the year 2002. By EIA assay, in 1042 samples (42.62%) Rotavirus was determined as positive. The virus existed around all the year, concentrated in Jan, Feb, March, Apr and July, affected commonly the children aged 3-35 months. From 154 genus of rotavirus, G type, G9 genus is the most common (90 genera). In addition, G2 (21 genera), G1 (19 genera), G4 (12 genera) and mixing types and unidentified types (12 genera) were also detected
Rotavirus ; epidemiology ; Diarrhea

Collecting stools sample from 232 children with acute diarrhoea, who treated in hospitals. Using diagnose immune enzyme EIA bio-product branch defined that reason of the disease is rotavirus. In 10 typies of rota G1P8 that cause of disease in Vietnam only have suitable and develop 4 typies in renal cell combination of Africa Rhesus monkey are G1P8 that signed 2001019423-11, 2001019427-15, 2001019428-17, 2001019432-20
Rotavirus ; Diarrhea ; Child

Whole studied poliomyelitis vaccine bulk lots had had sensitivity in high temperature and low concentration of NaHCO3. They all met WHO standards
Poliomyelitis ; Antigens, Bacterial ; Reference Standards

Background: \r\n', u'Rotavirus is the major important cause of infectious infantile gastroenteritis and diarrhea, especially the children aged 6 months to 24 months. Some efforts made to provide safe water and cultivate the hygiene have not reduced the diarrhea caused by rotaviruses. Develope a vaccine agaisnt this virus is of high importance. A question about the production of this kind of vaccine targeted athow to make human rotaviruses strains adapt cultured cells.\r\n', u'Objectives\r\n', u'To determine the multiplicity of infection (MOI) of human rotaviruses strains in vero cell.\r\n', u'Subjects and method: \r\n', u'The multiplicity of infection of Human rotaviruses strains (G1 P8, G1P4, and G4P6) were selected and the CDC- Atlanta US supplied the vero cell.Use vero cells cultivate, temper the vero cell and, applying direct immunofluorescence to determine the multiplicity of G1P8, G1P4, C4P6.\r\n', u'Results:\r\n', u'The MOI of G1P8, G1P4 and C4P6 are 0.02 ffu/cell, 0.15ffu/cell, and 0.35 ffu/cell, respectively.\r\n', u'Conclusion:\r\n', u'Using the vero cell to multiple the rotaviruses is the effective part during the production of vaccines against rotaviruses. The MOI of human rotaviruses strains G1P8, G1P4 and G4P6 are in order:0,02 ffu/cell, 0,15ffu/cell, and 0,35% ffu/cell. \r\n', u'
Rotavirus ; Vero Cells ; Cell Culture Techniques ;