OBJECTIVE:To establish the method to determine the concentration of human plasma felodipine by HPLC-MS.METHODS:The HPLC separating system was adopted and the mobile phase was a mixture of methanol-water-acetic acid with a flow rate at1ml/min and split ratio at2∶1,the fixed phase was Hypersil C 18 column;The mass spectrometric de-tection system and AP-ESI positive ion model were adopted with the atmosperic pressure at0.054Pa(400psi),the protecting gas at N 2 10L/min,capillary voltage at4000V,the ion source temperature at100℃and the fragment voltage at220V,the ions collected by SIM were felodipine m/z411(M+Na),Nimodipine m/z441(M+Na).RESULTS:The linear concentration range for felodipine was0.2～50ng/ml with a minimum detection limit at0.15ng/ml,the recovery was above90.93%with both the intra-day RSD and the inter-day RSD at less than9.8%(RSD

[Summary] The isolated dissection of the superior mesenteric artery ( IDSMA) was once considered to be a rare disease .In recent years , a growing number of cases of IDSMA have been reported , especially by Chinese authors , because of the increasing use of contrast-enhanced computed tomography scans .To understand the real domestic status of IDSMA , the Chinese-language literatures were searched, and the clinical characteristics and treatment of IDSMA were analyzed .

Objective To study the characteristics of breast disease and the effect of differentating diagnosis of breast disease under fiberoptic ductoscopy(FDS). Methods 50 cases of breast disease were examined by FDS. Results There were hyperplasia of mammary glands in 31 cases; chronic mastitis in 5 cases; ductal ectasia in 7 cases and breast cancer in 7 cases by FDS in this series. The accord rate of pathological diagnosis with FDS was 98%,and with clinical diagnosis was 76%.Conclusions FDS is a very important and new method in the diagnosis of breast disease .FDS can detect early breast cancer. It can be used as intervening treatment for some intraductal papilloma and chronic inflammatory disesase.FDS has precis location and qualitation effect for diagnosis of breast disease. FDS will greatly influence the diagnosis and treatment of breast cancer.

Paroxysmal supraventricular tachycardia (PSVT) is one of the frequent cardiovascular diseases.In recent years, cryoablation has become a research hot spot for PSVT treatment.Theoretically, its operability and safety are both better than those of radiofrequency ablation.Cryoablation can significantly reduce risk of ablation complications, but how to reduce recurrent rate after cryoablation is still a problem in the future.The present article made a review on main therapeutic methods of PSVT, especially application of cryoablation.

There is rapid growth of pediatric surgery service in China in the recent years. A survey by the China Association �A gainst �Epi�lepsy underta�ken in �June ����� 2006���� on the ma�jor cities in different parts of the country showed a dramatic increase particularly in years 2004 and 2006. Surgery for temporal lobe epilepsy accounts for half of the operation. Surgery has an important role in the treatment of drug resistant pediatric epilepsy. With large population and vast geographical spread, it is important to further develop pediatric epilepsy surgery service in China

The earliest activities related to epilepsy surgery in China ma�� � y be tra�ce��d to the 19���50s an�� �� d 60s��. A�ter a lull during the cultural revolution, there was resurgence of epilepsy surgery from the 1980s, and rapid development from 2000. It is estimated that app�roximately 150- ���200 p hysi�cians �currentl�� � y pra�cti�ce epilepsy surgery in the country.�� In year 2��� 005, there were a total of 2,��500 �cases of e�pile�ps��y o�perate��d in whole of �China, close to half in Beijing, Tian�jin, �Shanghai, �Chongqing an��d Guang�zhou, an�� a �uarter in Beijing. There are three Chinese epilepsy surgery journals, 5 monographs on epilepsy surgery, and “��Epilepsy surgery” edite�� ��� ��� �� ��� ��� d by HO Luders has also �been translate��d into �Chinese��.

Clinical significance of ovarian cancer marker COX 1 was studied . The data were colected from 97 cases of ovarian benign and malignant orarian tumors diagnosed by pathology . A sandwich enzyme immunoassay procedure was designed for quantitative determinations of COX 1, and CA125 was detected. 52 78% of COX 1 was found to be positive in ovarian cancer group ( P

Child ; Hemofiltration ; Humans ; Male ; Paraquat ; poisoning ; Poisoning ; therapy ; Treatment Outcome

Objective To study the main causes of hearing disability in Beijing residents. Methods The second national sample survey on disability of China was carried out in Beijing on April 1,2006. Muhiphase, stratified, proportional and cluster samplings were adopted to investigate 74 795 residents in Beijing, including 36 753 males and 38 042 females. 55 773 were urban residents while 19 022 from rural areas. The method of descriptive study was used to analyze the data of hearing disabitity in Beijing. Results Top five causes of hearing disability (including multiple disabilities) in Beijing were presbycusis, unknown, systematic diseases, noise induced, and odds media. The prevalence was 1.10%, 0.30%, 0.30%, 0.20% and 0.17%, respectively. Heredity, unknown and others were the main causes of children under 0～14 years. Unknown, ototoxicity, and otitis media were the main causes of people aged 15 ～59 years. Presbycusis, systematic diseases, noise induced and unknown were main causes of people aged 60 years and older. Presbycusis was the top cause of hearing disability for male and female residents as well as people live in urban and rural areas in Beijing. Conclusion Presbyeusis, unknown, systematic diseases, noise induced, and otitis media were main causes of hearing disability (including multiple disabilities) in Beijing.

Adenosine ; metabolism ; Adenosine Kinase ; metabolism ; Adenosine Triphosphate ; metabolism ; Animals ; Brain ; drug effects ; metabolism ; Diet, Carbohydrate-Restricted ; methods ; Diet, Ketogenic ; Energy Metabolism ; Epilepsy ; diet therapy ; metabolism ; Humans