Objective To explore the clinical significance of serum HGF level changes in gastric cancer pa-tients. Methods 60 cases of patients who operatied and clinicopathological confirmed gastric cancer, were detected serum HGF level by enzyme linked immunosorbent assay(ELISA) .40 cases were administered radical gastrectomy,20 cases of patients who had distant metastasis and loss operative chance were detected 7 days and 3 months preopera-tive,postoperative serum HGF level respectively. At the same time 40 cases of chronic gastritis patients and 40 cases of healthy examination people were selected as control group. Results Serum HGF level in patients with gastric canc-er was significantly higher than that of chronic gastritis and normal controls(P <0. 01). The serum HGF level of pa-tients with tumor vascular invasion significantly increased(P <0. 05). The patients with lymph node metastasis,serum HGF level was significantly higher than that patients with no lymph node metastasis(P <0. 05). The serum HGF level of patients with liver metastasis was significantly higher than that patients with no liver metastasis(P <0. 05). The se-rum HGF level of gastric cancer patients whose maximum diameter of the tumor ≥3cm were significantly higher than that maximum diameter of the tumor <3cm(P<0. 05). According to TNM staging of gastric cancer,the serum HGF level of Ⅰ phase and Ⅱ phase patients were significantly lower than that Ⅲ phase and Ⅳ phase patients(P <0.05).The serum HGF level of gastric cancer patients had no significant correlation with sex ,age and histological type(P <0.05). The serum HGF level of 40 cases of patients who were 1 week after surgery were significantly decreased,the patients who were 3 months after surgery were more obvious(P <0. 05 and P <0. 001). Conclusion HGF may play an important role in gastric cancer occurrence and development. The detection of serum HGF level can become an im-portant index for monitoring the degree of gastric cancer and prognosis of patients.

OBJECTIVE:To evaluate the methodological bias of systematic review and the reliability of counclusions of tradi-tional Chinese medicine (TCM) combined with transarterial chemoembolization in the treatment of primary liver cancer,and pro-vide evidence-based reference. METHODS:Retrieved from Cochrane Library,PubMed,Guideline,CBM,CJFD and Wanfang Da-tabase,high quality systematic review about TCM combined with transarterial chemoembolization in the treatment of primary liver cancer was collected for reevaluation. RESULTS:Totally 12 systematic review were involved. Results showed TCM combined with transarterial chemoembolization can improve tumor’s objective response,prolong the survival time of patients,improve life quali-ty,reduce postoperative adverse reactions with no serious adverse reactions reported related to TCM in the treatment of primary liv-er cancer. CONCLUSIONS:The clinical efficacy of TCM combined with transarterial chemoembolization is superior to transarterial chemoembolization alone in the treatment of primary liver cancer,with better safety.

Objective To study the association of single nucleotide polymorphisms (SNPs) of solute carrier family 22 member 4 (SLC22A4) and SLC22A5 genes with Crhon's disease (CD) in Chinese Han nationality. Methods SNPs in the entire coding region of SLC22A4 and SLC22A5 genes were screened by direct DNA sequencing in 80 CD patients and 80 healthy subjects, and statistical in Han population. Five SNPs were found in entire coding region (2 in SLC22A4 gene and 3 in distribution of the alleles and genotypes of SLC22A4 and SLC22A5 polymorphisms between CD patients and healthy controls. Conclusion There is no correlation of SLC22A4 and SLC22A5 with CD in Chinese Han nationality.

Objective To assess the reliability and validity of the Pittsburgh sleep quality index (PSQI) .Methods The cross-sectional survey was conducted in the outpatient department of this hospital .544 insomnia and non-insomnia voluntary participants (18-65 years old) from July to October 2011 were assessed with PSQI and the insomnia severity index (ISI) ,which were randomly delivered to them .Results The test-retest reliability of PSQI was 0 .994 .The split-half reliability coefficient of PSQI was 0 .824 . The overall Cronbach′s alpha coefficient was 0 .845 .The confirmatory factor analysis showed that the fitting indicators for the χ2/df ,root mean square error of approximation(RMSEA) ,non-normed fit index(NNFI) ,comparative fit index(CFI) and goodness-of-fit index(GFI) were 4 .83 ,0 .09 ,0 .96 ,0 .98 and 0 .97 respectively .The correlation coefficient between the PSQI and ISI was 0 .842 . The correlations coefficients between PSQI with the questionnaire type ,interview date and interview department were -0 .046 , 0 .276 and -0 .331 .Grouping by individuals with or without insomnia ,the area under the receiver operator characteristic curve (ROC) for PSQI was 0 .944(95% CI:0 .925-0 .963) .Conclusion PSQI has good reliability and validity ,and could be used for the sleep quality comprehensive evaluation in the patients with insomnia .

AIM:To discuss the main clinical characteristics of current drug eruption for reference of prevention and treatment of drug eruption.　METHODS: Two hundred and thirty-three with drug eruption in 1999 were collected, and their clinical characteristics, such as age, main causative drugs and eruption types, etc. were analysed.　RESULTS: The age of current drug eruption increased. Antibiotics was the most common causative drug, while antiinflammatory analgestics, serum and vaccine, traditional Chinese medicine were next to it. Scarlet fever-like or measles-like type was the most common eruption type, while urticaria type, erythema multiforme type and fixed type were next to it.　CONCLUSION: Clinical physicians shoud inquire alergic history of patients thoroughly and avoid unnecessary drugs, so as to decrease the occurrence of drug eruption.

Objective To discuss the diagnostic value of high frequency ultrasound on lesions of knee traumas.Methods Ninty-eight cases with post-traumatic knee injuries were examined by high frequency ultrasound,and the characteristics of changing ultrasonogram were observed and comparing with surgical results and MR.Results Of the 98 cases with post-traumatic knee injuries,there were 57 cases with effusion and hematocele in knee joint,among which there were 41 cases with collateral ligaments and meniscus injuries,the diagnostic coincidence rate was 93.3% in tendon tear and complete tendon fracture(compared with MR),91.7% in partial and complete muscle tear(compared with MR),67.7% and 71.4% in collateral ligaments and meniscus injuries(compared with surgical results).Conclusions High frequency ultrasound can be used as a conventional imaging examination method for post-traumatic knee injuries.

Chondrodysplasia Punctata ; congenital ; Humans ; Infant, Newborn ; Male

Ectodermal Dysplasia ; classification ; diagnosis ; Humans ; Infant ; Male ; Patient Care

Purpose To evaluate the correlation between neovascularization in femoral artery plaque and cerebral infarction by using contrast enhanced ultrasound,and to assess the predictive value of femoral artery plaque stability in cerebral infarction and its clinical significance.Materials and Methods Sixty-two patients with femoral artery hypoechoic plaques were retrospectively studied.The patients were divided into cerebral infarction group and control group,and all of them underwent two-dimensional ultrasound and contrast enhanced ultrasound.The parameters such as the maximum thickness,the stenosis rate of cross-sectional area,the enhanced intensity (EI),the area under the curve (AUC) and the grading of the neovascularization in the plaque were detected and compared.Results The EI and AUC of the cerebral infarction group were higher than those of the control group,the differences were statistically significant (P＜0.05).Many grade Ⅲ and Ⅳ neovascularizations were found in cerebral infarction group,while the grade Ⅰ and Ⅱ neovascularizations were mostly in the control group,the difference between the two groups was statistically significant (P＜0.05).There were no significant differences in the maximum thickness and stenosis rate of cross-sectional area between the two groups (P＞0.05).The neovascularization grade,EI and AUC in the plaque were positively correlated with cerebral infarction (r=0.331,0.416 and 0.410,P＜0.05).Conclusion ① The chara-cteristics of femoral artery hypoechoic plaques in patients with cerebral infarction are rich of neovascularization and instable by using contrast-enhanced ultrasonic imaging.② The neovascularization of femoral artery hypoechoic plaque has positive correlation with cerebral infarction,which may provide a new method for clinical prediction and prevention of intracranial atherosclerosis.

Objective To investigate the role of TLR9 in the glomerulonephritis through observing the changes of Toll-like receptor 9 in the glomerulo nephritis kidney tissue with or without CpG-ODN stimulation .Methods Wistar male rats were randomly divided into normal group(N),nephritis model group(M),CpG-ODN group(CpG) and GpC-ODN group(GpC).The urine samples were collected at 2,4,6 and 8 weeks after treatment,respectively.Blood samples were collected at the end of the last urine sample was collected,and the kidney tissue was collected,then the rats were killed.24 h urine protein was measured by Coomassie light blue technique.Serum album and renal function were determined by serological method.The pathologic changes of kidney were observed by light microscope and NF-?B p65 expression was detected using immunohistochemystry,RT-PCR was performed to detect the expressions of TLR9,INF-? and IL-6 mRNA.Results The expression of TLR9 was lighter in group M,and significantly increased after CpG-ODN stimulation compared with group M.Furthermore,24 h urine protein excretion was markedly increased,serum album was markedly decreased.The histopathologic changes of kidney were more severe.The mesangial cells(MCs) proliferated diffusifully in midrange and wide range,some of the glomeruli formatted cellularity crescent,micrangium loop was limitted,mononuclear macrophile cells were seen in the mesangial region.Conclusion Inflammatory factors mediated by TLR9 can deteriorate the biochemical and histopathologic changes.The immunologic reaction mediated by TLR9 is one of the mechanisms for the glomerulonephtitis' progression.