Child ; Humans ; Mycoplasma pneumoniae ; Pneumonia, Mycoplasma

Objective To assess the effect of 5-azacytidine (5-AZ) on the expressions of atrial natriuretic peptide (ANP) and β-myosin heavy chain (β-MHC) genes in cultured rat bone marrow stromal cells (BMSCs). Methods Neonatal rat cardiomyocytes and adult rat BMSCs were isolated and cultured. BMSCs were incubated with 5-AZ and (or) myocardiocytes culture medium. RT-PCR was used to measure the expressions of cardiac ANP and β-MHC genes. Results Normally cultured rat BMSCs didn't express cardiac ANP or β-MHC. After inducing by 5-AZ, rat BMSCs expressed ANP or β-MHC. The myocardiocytes culture medium enhanced expression of β-MHC, but didn't affect the expression of ANP. Conclusions The 5-AZ induces the rat BMSCs to express either cardiac ANP or β-MHC, and the former could be enhanced by cardiomyocytes culture medium.

The velocity of blood in vessels is an important indicator that reflects the microcirculatory status. The core of the measurement technology, which is based on spatiotemporal (ST) image, is to map the cell motion trace to the two-dimensional ST image, and transfer the measurement of flow velocity to the detection of trace orientation in ST image. This paper proposes a trace orientation measurement algorithm is based on Randomized Hough Transformation and projection transformation, and it is able to estimate trace orientation and flow velocity in noisy ST images. Experiments showed that the agreement between the results by manual and by the proposed algorithm reached over 90%.
Algorithms ; Blood Flow Velocity ; Humans ; Microcirculation ; Spatio-Temporal Analysis

Objective To explore the diagnostic value of serum myoglobin (Mb) and cardiac troponin (cTn) in patients with acute myocardial infarction (AMI) by reviewing and evaluating the corresponding references with evidence-based medicine (EBM) theory.Methods All references (including papers,reviews and abstracts) of CNKI-CHKD,CMCC(1994-2005)and EMCC(1995-2005) were collected and retrospectively analyzed according to evidence based medicine (EBM) standard,with Mb cTn as the detected indices for diagnosing AMI.Results In the searched period of 1994-2005,123 papers were published (93 pieces were written in Chinese,and 30 were written in English). In most references,the authors set clinical control trial (CCT) (Chinese: 92.5%;English: 53.3%),blind control trial (Chinese: 19.4%;English: 10.0%) and/or random control trial (RCT) (Chinese: 9.7%;English: 16.7%). The respective reference ranges weren′t provided in 42 pieces of articles.Conclusion The analytic result of the references in home and aborad indicates combined detection of Mb and cTnI posseses favourite sensitivity and specificity,which contributes to the early diagnosis of acute myocardial infarction.

Objective　To study on effects of mVEGF and anti-VEGF antibody during cultured HUVEC proliferation in vitro.Methods　Endothelial cell proliferation was assayed using human umbilical vein endothelial cells stimulated with mVEGF and with CIA joint extracts and was used　　3　　H-TaR incorporation.Results　The anti-VEGF neutralizing antibody can inhibit the proliferation of HUVEC stimulated with mVEGF and with CIA joint extracts,whose suppression percents were 72.2% and 69.9%,respectively.Conclusion　mVEGF specifically promotes the growth of vascular endothelial cells.During early stage of CIA development,expression of VEGF in the joint increases and VEGF is expressed biologically active and can be inhibited by anti-VEGF neutralizing antibody.

The perineurial cells of the small nerve branch of the normal human abdominal wall are observed with electron microscope. The fibrous long-spacing bodies (FLS) are found within the interstitial substance of the endoneurium. 1. 2-5 layers of the perineurial cells surround the nerve fasciculus. The perineurial cells are squamous in shape and the cytoplasm contains microfilaments and pinocytotic vesicles. Each perineurial cell has an obvious basement membrane on its basal surface but the fibroblasts, whatever situated in the endoneurium or on the outer surface of the perineurium, has no basement membrane. Numerous desmosome and some gap junctions between the close attached perineurial cells are demostrated. The collagen fibrils between perineurial cells can be often shown. FLS bodies and collagen fibrils about 45 nm in diameter in the interstitial substance of endoneurium inside perineurial cells are demonstrated. In the connective tissue surrounding the outside of perineurial cells, the collagen fibrils of 80 nm in diameter can be seen, but no FLS bodies present there. 2. FLS bodies in the endoneurial matrix can be demostrated. Most of them closely associated with the basement membrane of the Schwann cells surrounding the unmyelinated nerves but no FLS bodies are found associate with those of the myelinated nerves. A few FLS bodies do not relate to basement membrane and they are invested only with the collagen fibrils. In the longitudinal sections, most of FLS bodies are in spindle shape of various sizes, their longitudinal axes parallel to that of the adjoining collagen fibrils. Sometimes the FLS bodies continue with the collagen fibrils are found. Occasionally, FLS body like a bridge locate between two Schwann cells and its dark bands continue to the basement membrane of the Schwann cells. Two or three FLS bodies may fuse together but their cross bands do not registered at the same level. In the oblique sections, FLS bodies are nearly rectangular in shape, with the cross bands shown, and their appearance do not show greaty difference from those in longitudinal sections. The periodicity of FLS bodies is about 133nm in length; the dark band in it is about 53 nm, which is made of the dense granular substance; the light band is about 80 nm, which is made of a network with approximated parallel microfilaments. There are no further crossstriated structures within the periods. Some problems, such as the role of the perineurial cells, the relationship of FLS bodies with basement membrane, are briefly discussed.

Diabetes associated large vascular disease is a major threat to type 2 diabetes mellitus,its main pathological mechanism is atherosclerosis.Carotid intima-media thickness (CIMT) detected using high resolution ultrasound technique can be used as an alternative indicator of subclinical atherosclerosis.It has been confirmed that it is a strong predictor of cardio-cerebrovascular diseases.This article focuses on the latest research progress of the CIMT-associated risk factors in patients with type 2 diabetes mellitus.

Objective To rapidly construct hepatitis B virus(HBV)adefovir(ADV)‐resistant strain(RT A181T)infectious clone by using SOE‐PCR ,to observe the expression of recombinant plasmids in Huh7 cells and to establish the in vitro cell model of HBV ADV‐resistant strain(RT A181T) .Methods The conservative primers were designed ,the full‐length HBV genome was amplified from serum in the patients with ADV‐resistant chronic hepatitis B .Then ,the 1 .3‐fold genome‐length infectious clone pHBV1 .3 was constructed by using the SOE‐PCR technique ,which was transfected into human hepatoma cells Huh7 cell line .ELISA ,Western Blot and Real‐time PCR were adopted to detect infectious cloning replication and expression ,meanwhile the antiviral drugs lamivu‐dine(LAM ) and ADV were used to verify the infectious clone copy and inhibition of expression .Results The HBV ADV‐resistant infectious cloning plasmids pHBV1 .3 was successfully constructed .This plasmid could be effectively replicated ,transcripted and ex‐pressed in Huh7 cell lines .LAM could effectively inhibit the replication and expression of the infectious clone ,while ADV could not inhibit the replication and expression of the infectious clone .Conclusion The constructed infectious clone plasmids pHBV1 .3(RT A181T ) of HBV ADV‐resistant strain can efficiently replicate and express protein in vitro ,its transfected cells can be used in the study of HBV replication mechanism and antiviral study .

Metoclopramide ( MCP ) is similar to Procainamide in structure. In rats anaesthetized by ip chloral hydrate 300 mg/kg, arrhythmia induced by BaCl 3 mg/kg was shown to be antagonized by injecting iv MCP 30 mg/kg. It was also found that MCP 10 mg/kg injected iv protected rabbits against a'rrhythmia induced by chloroform-adrenaline . In anaesthetized rats, MCP 30 mg/kg injected iv raised the dose of aconitine required to induce first arrhythmia, ventricular fibrillation ( VF ) and heart arrest, while in anaesthetized guinea pigs, the same amount of MCP injected iv showed no significant action on the dose of ouabain inducing these effects. Ventricular tachycardia produced by occlusion of coronary artery in rats could not be prevnted by injecting iv MCP 30 mg/kg. It was found that MCP has local anaesthesia action.