Objective　To study on effects of mVEGF and anti-VEGF antibody during cultured HUVEC proliferation in vitro.Methods　Endothelial cell proliferation was assayed using human umbilical vein endothelial cells stimulated with mVEGF and with CIA joint extracts and was used　　3　　H-TaR incorporation.Results　The anti-VEGF neutralizing antibody can inhibit the proliferation of HUVEC stimulated with mVEGF and with CIA joint extracts,whose suppression percents were 72.2% and 69.9%,respectively.Conclusion　mVEGF specifically promotes the growth of vascular endothelial cells.During early stage of CIA development,expression of VEGF in the joint increases and VEGF is expressed biologically active and can be inhibited by anti-VEGF neutralizing antibody.

Objective To rapidly construct hepatitis B virus(HBV)adefovir(ADV)‐resistant strain(RT A181T)infectious clone by using SOE‐PCR ,to observe the expression of recombinant plasmids in Huh7 cells and to establish the in vitro cell model of HBV ADV‐resistant strain(RT A181T) .Methods The conservative primers were designed ,the full‐length HBV genome was amplified from serum in the patients with ADV‐resistant chronic hepatitis B .Then ,the 1 .3‐fold genome‐length infectious clone pHBV1 .3 was constructed by using the SOE‐PCR technique ,which was transfected into human hepatoma cells Huh7 cell line .ELISA ,Western Blot and Real‐time PCR were adopted to detect infectious cloning replication and expression ,meanwhile the antiviral drugs lamivu‐dine(LAM ) and ADV were used to verify the infectious clone copy and inhibition of expression .Results The HBV ADV‐resistant infectious cloning plasmids pHBV1 .3 was successfully constructed .This plasmid could be effectively replicated ,transcripted and ex‐pressed in Huh7 cell lines .LAM could effectively inhibit the replication and expression of the infectious clone ,while ADV could not inhibit the replication and expression of the infectious clone .Conclusion The constructed infectious clone plasmids pHBV1 .3(RT A181T ) of HBV ADV‐resistant strain can efficiently replicate and express protein in vitro ,its transfected cells can be used in the study of HBV replication mechanism and antiviral study .

In order to continuously improve the level of English abstracts for medical postgraduates , this paper introduces the definition, purpose, types, contents and writing strategies of English abstracts .It also introduces the writing difference and evolution of the word abstractand the writing tips of all kinds of abstracts , especially the structured abstract .In reference to international and do-mestic writing criteria , it points out the problems which should be paid attention to in writing English abstracts and tried to develop the students′consciousness in improving their level of writing English abstracts so that their thesis will be better recognized and cited by in -ternational experts , thus promoting the international academic exchanges as well as the development of China ′s medical undertakings .

Objective To improve the diagnosis and treatment of hepatolithiasis associated with cholangio-caminoma.Methods Data of 28 cases of hepatolithiasis associated with intrahepatic cholangiocarcinoma from 2005 to 2008 in Li Huili Hospital of Ningbo University Medical School were collected and analysed.Results The diagnosis was established preoperatively in 8(28.6%)cases.Twenty-one(75.0%)patients were radically resected,5(17.9%)patients received palliative operation,and other 2(7.1%)patients received exploratory surgery.In the 24 patients followed-up closely,the median survival time was 30 months in radical surgery group,8 months in palliative operation group,whereas 2 exploratory surgery cases only survived 2 months and 4 months respectively.Conclusions Cholangiocarcinoma may be related to hepatolithiasis.Early diagnosis rate is low and prognosis is poor in the cholangiocarcinoma associated with hepatolithiasis.Early radical therapy for hepatolithiasis plays an important role in preventing cholangiocarcinoma occurrence.Palliative operation for cholangiocarcinoma may improve quality of live.

OBJECTIVE To understand operating room management conditions and put forward improvement methods at township hospitals.METHODS An observatory study was conducted in 9 township hospitals using the evaluation criteria based on the requirements of the Guidelines for Disinfection in Hospitals.Technical Guidelines for Prevention and Control of the Hospital Infection in Operation Departments and the Guidelines for Hand Hygiene of Medical staff in Medical Institutions.RESULTS Problems were found to a different extent in all of the 9 township hospitals:faults in rules and regulations,unreasonable layout technological process,unstandardized sterilization and disinfection and short knowledge about hospital infection.CONCLUSIONS Special attention to managment of operating room,improving consciousness,strengthening medical staff study and improving system are the key issues for improving the management level of operating room at township hospitals.

The velocity of blood in vessels is an important indicator that reflects the microcirculatory status. The core of the measurement technology, which is based on spatiotemporal (ST) image, is to map the cell motion trace to the two-dimensional ST image, and transfer the measurement of flow velocity to the detection of trace orientation in ST image. This paper proposes a trace orientation measurement algorithm is based on Randomized Hough Transformation and projection transformation, and it is able to estimate trace orientation and flow velocity in noisy ST images. Experiments showed that the agreement between the results by manual and by the proposed algorithm reached over 90%.
Algorithms ; Blood Flow Velocity ; Humans ; Microcirculation ; Spatio-Temporal Analysis

Objective To explore the diagnostic value of serum myoglobin (Mb) and cardiac troponin (cTn) in patients with acute myocardial infarction (AMI) by reviewing and evaluating the corresponding references with evidence-based medicine (EBM) theory.Methods All references (including papers,reviews and abstracts) of CNKI-CHKD,CMCC(1994-2005)and EMCC(1995-2005) were collected and retrospectively analyzed according to evidence based medicine (EBM) standard,with Mb cTn as the detected indices for diagnosing AMI.Results In the searched period of 1994-2005,123 papers were published (93 pieces were written in Chinese,and 30 were written in English). In most references,the authors set clinical control trial (CCT) (Chinese: 92.5%;English: 53.3%),blind control trial (Chinese: 19.4%;English: 10.0%) and/or random control trial (RCT) (Chinese: 9.7%;English: 16.7%). The respective reference ranges weren′t provided in 42 pieces of articles.Conclusion The analytic result of the references in home and aborad indicates combined detection of Mb and cTnI posseses favourite sensitivity and specificity,which contributes to the early diagnosis of acute myocardial infarction.

Objective To assess the effect of 5-azacytidine (5-AZ) on the expressions of atrial natriuretic peptide (ANP) and β-myosin heavy chain (β-MHC) genes in cultured rat bone marrow stromal cells (BMSCs). Methods Neonatal rat cardiomyocytes and adult rat BMSCs were isolated and cultured. BMSCs were incubated with 5-AZ and (or) myocardiocytes culture medium. RT-PCR was used to measure the expressions of cardiac ANP and β-MHC genes. Results Normally cultured rat BMSCs didn't express cardiac ANP or β-MHC. After inducing by 5-AZ, rat BMSCs expressed ANP or β-MHC. The myocardiocytes culture medium enhanced expression of β-MHC, but didn't affect the expression of ANP. Conclusions The 5-AZ induces the rat BMSCs to express either cardiac ANP or β-MHC, and the former could be enhanced by cardiomyocytes culture medium.

Parkinson's disease(PD) is a progressive neurodegenerative disorder harming mainly the elderly.Astrocyte may be involved in the pathogenesis of PD.However,it also plays a protective role in PD by releasing neurotrophic factors and glutathione to support the survival of dopaminergic neurons.Astrocyte may also release proinflammatory cytokines to promote the development of PD.

Heterotopic ossification is the abnormal formation of true bone within extraskeletal soft tissues, which frequently showing a predilection instead for fascia, tendons, and other mesenchymal soft tissues. This paper summarized differential types and quantities of regulatory proteins expression at different stages of heterotopic ossification by proteomics techniques, and screened differential expression of functional proteins at different time points. Further more, identificated and validated certain proteins, which helpful to discovery new regulatory proteins and explore the mutual relationships among the proteins. Proteomics has extensive applications in regulatory proteins research of post-traumatic heterotopic ossification, can further elucidate the mechanism of post-traumatic ectopic bone formation and search for new regulatory biomolecule regulatory sites.