Objective To get genetic transformated Erigeron breviscapus plantlet with the antiinsect and antifungi effects.Methods The plasmid with gene RBBI2-3 was produced by PCR,middle carrier PBS and plant carrier YY46.By using the particle gun to bombard E. breviscapus,genetic transformation was realized.It was done with the control experiment on bombarded materials,bombarding pressures,and Gent.sensitivity.Results The result of genetic transformation by using true leaves as bombarded material was the best;Ⅱ-type callus were the next in order;the roots,stems,and Ⅰ-type callus were the last in the result.The bombarding pressure of 8.96?10~6 Pa was the best to induce true leaves forming adventitious bud.The result of using the pressure of 7.58?10~6 Pa and 8.96?10~6 Pa to bombard Ⅱ-type callus did not show any difference and was next to true leaves.The best result is using 50 ?g/mL Gent to choose the E.breviscapus of genetic transformation.Conclusion The paddy RBBI2-3 transformed into the genetic transformation on E.breviscapus by particle gun bombarding is the best.The genetic transformated E.breviscapus plantlet has been obtained.

It is generally known that postoperative pain can cause many adverse clinical effects on the patients of orthopaedic surgery such as lack of exercises of the involved limb,atrophy of the related muscles,anchylosis,osteoparosis,et al,which will ultimately affect the patient's final recovery and living quality.This paper introduces some recent advanced theories about the orthopaedic postoperative pain diagnosis,evaluation and various kinds of treatments hoping to achieve more effective perioperative analgesia in orthopaedic surgery.

Humans ; Lymphoproliferative Disorders ; classification ; World Health Organization

Humans ; Multiple Myeloma ; diagnosis ; therapy ; Prognosis

China ; Humans ; Multiple Myeloma ; diagnosis ; therapy ; Translational Medical Research

Humans ; Multiple Myeloma

Objective:To explore effect of objective life status and psychological factors on the life satisfaction (SLS) in retired carders.Methods:Two hundred and forty-four retired carders were assessed with Quality of Life Inventory (QOLI), The Elderly's Coping Style Questionaire and Social Support Questionaire.Results:The correlation analysis showed that SLS was significantly related with OIQL, coping style, social support,and life expectation.Multiple stepwise regression analysis revealed that objective indicators about physical state and mental state from OIQL distancing style from positive coping and social support had significant effect on SLS.

Peroxisome proliferators-activated receptor-gamma(PPAR?),one of members of the nuclear hormone receptor superfamily of ligand-activated transcription factors,may affect the ocurrence and development of atherosclerosis(AS) through effective modulate lipocyte differentiation,improve glucose and lipoprotein decompensation and control inflammation reaction and so on.This review will focus on the recently development that relation on PPAR? and AS.

Objective To evaluate the effect of Liujunzi soup on the total course of disease and inflammatory mediators in patients with remission of allergic cough. Methods Eighty six patients with allergic cough were enrolled in our hospital from April 2011 to April 2012. The patients were randomly divided into observation group (n = 43) and control group (n = 43). The control group was given basic western medicine treatment, the observation group in the control group on the basis of another treatment of six gentleman soup. The levels of inflammatory cytokines, pulmonary function and adverse reactions were compared between the two groups in clinical efficacy, hospitalization time, total course of disease, IL-2, TNF-α and IL-6. Results The total effective rate was 90.70％ in the observation group was significantly higher than that in the control group (72.09％)( P<0.05). The duration of hospitalization (13.35 ± 3.24) d and the total course of disease (17.68 ± 4.53) d were significantly shorter than those in the control group (16.23 ±3.10) d, (20.35 ± 4.67) d (P<0.05). The levels of inflammatory factors and pulmonary function in the observation group were statistically significant compared with the control group (P<0.05). No adverse reactions occurred in both groups. Conclusion Liujunzi Decoction combined with western medicine can improve the level of inflammatory cytokines and lung function, shorten the length of hospital stay, the total course of disease and high safety, and have good clinical value.