In presnt experiment, animal models for hyperthyroidism and hypothyroidism were made, and using radioimmunoassay, the immunoeactive leucine-enkephalin (Ir-LEK) levels of such discrete brain areas as hypothalamus, striatum, cerebral cortex(frontal lobe) and whole pituitary were determined in different groups. The results obtained indicated that both hyperthyroidism and hypothyroidism induced significant changes of concentration in CNS Ir-LEK. It was found in hypothyroid group that concentration of Ir-LEK in hypothalamus, cerebral cortex and pituitary increased significantly against the control (P

OBJECTIVE:To establish a method for the determination of related substances in Levo-cetirizine hydrochloride chewable tablet. METHODS:RP-HPLC was performed on the column of Venusil XBP-CN with mobile phase of acetonitrile-0.05 mol/L Sodium dihydrogen phosphate solution(pH value adjusted to 3.0 by phosphoric acid)(60∶40,V/V)at a flow rate of 1.0 ml/min, the detection wavelength was 230 nm,column temperature was 25 ℃,and volume injection was 20 μl. RESULTS:Levo-cetirizine hydrochloride chewable tablet showed good separation with related substances;the linear range of levo-cetirizine hydrochloride was 1.2-2.8 μg/ml(r=0.999 9);the quantification limit and detection limit was 1.4 ng/ml and 0.3 ng/ml,respectively;RSDs of preci-sion,stability and reproducibility tests were lower than 1%;recovery was 96.3%-105.0%(RSD=1.7%,n=9);the contents of re-lated substances were no more than 0.17%. CONCLUSIONS:The method is simple,sensitive,reproducible,accurate and reli-able,and can be used for the determination of related substances in Levo-cetirizine hydrochloride chewable tablet.

Bacterial Infections ; epidemiology ; etiology ; Cerebral Hemorrhage ; diagnosis ; etiology ; therapy ; Craniocerebral Trauma ; diagnosis ; etiology ; therapy ; Female ; Fetal Membranes, Premature Rupture ; Gestational Age ; Humans ; Infant, Newborn ; Infant, Premature ; Infant, Premature, Diseases ; diagnosis ; etiology ; therapy ; Pregnancy ; Risk Factors ; Tomography, X-Ray Computed

The therapy of removing beat and toxic,strengthening the body to cultivate the fundamental is the mainstream of treating cancer in traditional Chinese medicine up to now,and the therapy of warming yang has been rarely involved.While the pathogenesis of tumor is spleen and kidney yang deficiency in root,and phlegm,stasis,and poison entangled with each other at the secondary position; the main therapeutical principle of tumor is simultaneous application of purging-tonifying therapy while focusing on protecting yang.The author believes that the treatment of cancer should pay attention to the whole concept,especially warming yang for patients with advanced tumors.The therapy of warming yang is an effective way to get rid of phlegm and blood stasis.Patients after radiotherapy and chemotherapy should be paid more attention to warming yang.

Deifnitive treatment for locoregionally advanced squamous cell carcinoma of the head and neck region (HNSCC) is challenging, and usually require multidisciplinary efforts involving surgery, radiotherapy, and chemotherapy. Although surgery followed by radiation or chemoradiation therapy remains the standard treatment for resectable disease, combined chemoradiation therapy provides an effective option with organ spearing potential. In addition, combined chemoradiation therapy is the only treatment option for non-metastatic advanced HNSCC. Recently published results from TAX323 and TAX324, two important randomized clinical trials on the efficacy of induction chemotherapy using docetaxel based regimen, showed that induction chemotherapy using TPF can signiifcantly improve patients’ survival as compared to the conventional PF regimen. However, whether TP or TPF induction chemotherapy should be combined with concurrent chemoradiation and considered as part of the standard treatment regimen remains controversial, and requires support from the results of well-designed randomized clinical trial.

AIM To study the effects of RAPA on lymphocyte proliferation induced by mitogen and in mixed lymphocyte reaction(MLR), and the effects on T subpopulation and the content of IL 2,IFN ?,TNF ? in mice. METHODS Lymphocyte proliferation induced by mitogens and MLR were detected with MTT colometeric assay. T cell subsets were measured with fluorescence activated cell sorter (FACs). IL 2,IFN ? and TNF ? were detected by ELISA and biological assay. RESULTS RAPA inhibited murine lymphocyte proliferation induced by Con A, PHA or LPS, and also inhibited lymphocyte proliferation stimulated in MLR. RAPA had no significant effect on murine spleenic T lymphocyte subpopulation. RAPA inhibited ConA induced production of IL 2 of IFN ? by murine splenocytes, but had not inhibited LPS induced TNF ? production by murine peritoneal macrophage. CONCLUSION The immunosuppressive mechanism of RAPA is distinct from CsA.

Observating 13 neonates who accepted mechanical ventilating reatment for respiratory failure, the important methods about the nurse of the neonatal respiratory tract were summarized. The clinical nursing care, the nutritionsurpporting,and the reducing complication of the mechanical ventilation in neonates were emphasized.

Objective:To investigate the effects of rapamycin on allograft rejecti on and immune response in mice. Methods:The heterotopic ear-hea rt grafting or sk in grafting were done in mice. The delayed type hypersensitivity (DTH) response i nduced by dinitro-fluorobenzene (DNFB), the production of hemolysin of mouse sensitized by sheep red blo od cell (SRBC) and the neutral red phagocytosis of the peritoneal macrophage wer e also tested in mice. Results:RAPA significantly blocked allograft reject ion of heart and skin, markedly inhibited DTH response and decreased the production of hemolysin,but had no significant effect on the neutral red phagocytosis of the p eritoneal macrophage. Conclusion:RAPA potently blocked allograft rej ections in mi ce and suppressed cellular and humoral immune response, but had no significant e ffect on phagocytoses of macrophage.

Objective To investigate the clinical significance of the changes of hemodynamics and electrolyte during orthotopc liver transplantation.Methods We studied 9 patients undergoing elective orthotopic liver transplantation without venovenous bypass,drew out arterial blood for monitoring arterial blood gas tensions,Na~+,K~+,Ca~(2+) and observed changes in hemodynamics and electrolyte.Results In most patients,arterial pressure significantly decreased at the prophase of the anhepatic phase,and tended to normal range in other phases.The concentration of calcium maintained a low-level during the whole surgery.During the prophases of the anhepatic phase and neo-hepatic phase,there was a light hyperkalemia,and the concentration of natrium slightly increased since 30 minutes after the anhepatic phase and neohepatic phase,but insensibly exceeded normal range.With resuming of the stability of hemodynamics,these changes had followed.Conclusion We shoud give our attention to correct the occurrence of the hypocalcemia during the forepart of the anhepatic phase and neo hepatic phase,and care for the hyperkalemia 5 minutes after the block of liver blood purveyance and revascularization of liver.The lock of body capability shoud be recruit duly according to the lose of blood and hemacytometer changes.In order to prevent serious academia and maintain the stability of hemodynamics and electrolyte,vasoconstrictor shoud be used carefully during the anhepatic phase.

The lack and absence of knowledge in humanities and medical ethics among nursing staff have a negative influence on the mutual communication between nurses and patients and the implementation of humanistic nursing.The implementation of humanistic nursing can improve the general quality of nursing staff,especially their knowledge in humanities and medical ethics,and increase patients' satisfaction towards medical service.Meanwhile,it can also upgrade hospital's overall image,and increase social and economic profits of hospitals as well.