Objective:To study the expressions of hepatocyte growth factor (HGF) and basic fibroblast growth factor (bFGF) in fresh and two preserved amniotic membranes (AM),and to explore their biological vitality.Methods:The amniotic membranes from the healthy puerperia with abdominal delivery were randomly divided into three groups:fresh group,DMEM/glycerol preserved group at -80℃ and glycerol preserved group at 4℃ (for 1 and 3 months).Immunohistochemical method was used to investigate the changes in the expressions of HGF and bFGF among the three groups.Results:HGF and bFGF were distributed in the epithelium of AMs mostly.The expressions of HGF in both preserved AMs for 1 and 3 months decreased significantly compared with fresh AM (P

Humans ; Multiple Myeloma ; Neoplasm Recurrence, Local

Objective　To study on effects of mVEGF and anti-VEGF antibody during cultured HUVEC proliferation in vitro.Methods　Endothelial cell proliferation was assayed using human umbilical vein endothelial cells stimulated with mVEGF and with CIA joint extracts and was used　　3　　H-TaR incorporation.Results　The anti-VEGF neutralizing antibody can inhibit the proliferation of HUVEC stimulated with mVEGF and with CIA joint extracts,whose suppression percents were 72.2% and 69.9%,respectively.Conclusion　mVEGF specifically promotes the growth of vascular endothelial cells.During early stage of CIA development,expression of VEGF in the joint increases and VEGF is expressed biologically active and can be inhibited by anti-VEGF neutralizing antibody.

Objective To compare the clinical effect of C3F8 and air tamponade after vitrectomy for the treatment of idiopathic macular hole (IMH).Methods A total of 54 eyes of 54 patients with IMH that had undergone 23G pars plana vitrectomy with internal limiting membrane peeling were retrospectively studied.All patients received optical coherence tomography ()CT) examination and the best corrected visual acuity (BCVA).They were divided into 2 groups.26 eyes in group A were filled with air and 28 eyes in group B were filled with C3F8.In group A,6 eyes at stage Ⅱ,20 eyes at stage Ⅲ,the minimum diameter (Dmin) of macular hole in 14 eyes was less than 400 μm,and in the other eyes was larger than 400 μm.In group B,10 eyes at stage Ⅱ,18 eyes at stage Ⅲ,the Dmin of macular hole in 15 eyes was less than 400 μm,and in the other eyes was larger than 400 μm.The differences of age,course of the disease,BCVA,fundus diameter,Dmin,height,index,diameter of outer retina diameter (Dord) between the two groups were not significant (P＞0.05).The basic data before surgery and the closure rate,BCVA,Dord 1 month after surgery between two groups were compared.Results At 1 month after surgery,the IMH closure rate was 100.0％ in group A and 92.9％ in group B,the difference between these two groups was not significant (P=0.491).The closure rate of eyes with Dmin＜400 μm were both 100.0％ in two groups,and the closure rate of eyes with Dmin＞400 μm were 100.0％ in group A and 84.6％ in group B.There was no statistically significant differences between two groups (P =0.480).The mean BCVA of two groups were 0.35±0.22 and 0.33 ±0.16 respectively.The mean Dord were (782.2 ± 478.0) μm and(792.1 ± 432.7) μm respectively.All cases got better BCVA (t=-7.310,-10.506;P＜0.01) and shorter Dord (t=6.704,7.770;P＜0.01).But there was no statistically significant differences between groups 1 month after surgery in BCVA and Dord (t =0.381,0.800;P =0.705,0.937).Conclusion Air tamponade after vitrectomy has the same efficacy as C3 F8 tamponade in the treatment of IMH.

?AlM: To observe the changes of corneal topography and astigmatism et al with various duration of closing eyes and sleep;and study the impact of closing eye and sleep causing physiological hypoxia on corneal topography.
? METHODS: Sixteen volunteers were selected ( 32 eyes), 22-33 (26. 19 ± 3. 95) years old, without refractive errors and other eye diseases, as well as never wearing corneal contact lens, including 10 male eyes and 22 female eyes. The main parameters received corneal simulation K value, corneal astigmatism, corneal irregular measure ( ClM ) and the shape factor ( SF ) by Zeiss corneal topographer. They were measured before and after closed eyes 10, 20, 30, 60, 120min; before sleep and after over 6h sleep, they were detected immediately as soon as they opened eyes after 10, 20, 60, 120min.
? RESULTS: During closing eyes, there were no statistical significance on the changes of corneal topography, corneal astigmatism, and SF. After opening eyes and over 6h sleep, there were no statistical significance on the changes of corneal topography, corneal astigmatism, ClM, and SF.
?CONCLUSlON:Closing eyes and sleep don’t injure the cornea substantially, so corneal topography and corneal astigmatism don’t mainly change.

AIM: To investigate the effect and mechanism of Mingjing decoction combining with argon laser on the levels of plasma vascular endothelial growth factor (VEGF) in patients with diabetic retinopathy(DR).
METHODS:The 66 patients(118 eyes)with DR at Ⅲ ~ Ⅴstage were randomly divided into two groups (treatment group and control group). The control group (33 patients with 56 eyes ) was treated with argon laser, and the treatment group (33 patients with 62 eyes) was treated with mingjing decoction combining with argon laser, and a blank group(30 eyes) was set up. The levels of plasma VEGF were detected by enzyme linked immunosorbent assay (ELISA). The results of best corrected visual acuity and fundus fluorescein angiography(FFA) were analyzed, as well as detected glycosylated hemoglobin ( HbA1c), blood coagulation function and the function of liver and kidney.
RESULTS: Patients with DR had significantly risen plasma VEGF before treatment when compared with blank control group ( P < 0. 05 ). After 3mo of combined treatment, the levels of plasma VEGF significantly reduced and HbA1c decreased in patients treated with mingjing decoction and argon laser, there were statistically significant difference compared to control group ( P < 0. 05 ). In the treatment group, the best corrected visual acuity and FFA were significantly improved in patients treated with mingjing decoction combining with argon laser compared to patients treated with argon laser alone after 3mo of combined treatment. CONCLUSION: Mingjing decoction combining with argon laser for DR can effectively reduce the level of plasma VEGF, stabilize blood sugar levels, improve the function of retina, and delay DR progresses.

Astragaloside Ⅳ content in species of Radix Astragali seu Hedysari of Chinese materia mediea has been determined with dual wavelength,reflect zig-zag scanning,external-standard dual-point methods. The average recovery and coefficient of variation(CV)are 99.15% and 3.71%(n=6),respective- ly.

Multiple myeloma is a malignancy of plasma cells,which is still incurable now.Despite dramatic improvement of the response rate and overall survival have been made due to the application of target therapy and stem cell transplantation,the treatment effect is difficult to maintain,nearly all patients will eventually relapse and become refractory.At relapse,the challenge is to select the appropriate treatment for each patient.The decision will be both disease-dependent and patient-dependent.

Objective To observe the efficacy and prognosis of aconite-cake-partitioned moxibustion plus acupuncture in treating peptic ulcer.Method Thirty-four patients in the treatment group were intervened by aconite-cake-partitioned moxibustion plus acupuncture, while thirty-three patients in the control group were by Omeprazole Enteric-coated Capsules. The clinical efficacy, the effect on ulcer under gastroscope and Hp, and the therapeutic efficacy 12 months after the intervention were evaluated.Result The total effective rate was 91.2% in the treatment group versus 72.7% in the control group, and the difference was statistically significant (P<0.05). For ulcer by gastroscopy, the total effective rate was 85.3% in the treatment group versus 69.7% in the control group, and the difference was statistically significant (P<0.05). After intervention, the Hp level didn’t show significant change (P>0.05). The relapse rate of the control group was higher than that of the treatment group 12 months after the intervention.Conclusion Aconite-cake-partitioned moxibustion plus acupuncture can produce a higher efficacy than Western medication in treating peptic ulcer.

Objective To observe the clinical efficacy of acupuncture-moxibustion in treating endometriosis (EM). Method Fifty EM patients were randomized into two groups, 25 cases in each group. The treatment group was intervened by acupuncture-moxibustion, while the control group was intervened by oral administration of Mifepristone tablets. Abdominal pain, pelvic lump, and relevant indicators were observed before and after the treatment, and the therapeutic efficacies were compared.Result After 6-month treatment, symptoms including abdominal pain obviously subsided in the treatment group and the total effective rate was 92.0%, versus 52.0% in the control group, and the between-group difference was statistically significant (P<0.01). The pelvic lump size was significantly changed after the treatment in both groups (P<0.05), suggesting that the lump size became smaller in both groups after the treatment;there was a significant difference in comparing the pelvic lump size between the two groups after the treatment (P<0.05), indicating that the lump size was reduced more significantly in the acupuncture-moxibustion group. The level of serum CA125 changed significantly after the treatment in both groups (P<0.05), suggesting that serum CA125 dropped in both groups after the treatment; there was a significant difference in comparing the level of serum CA125 between the two groups after the treatment (P<0.05), indicating that serum CA125 dropped more significantly in the acupuncture-moxibustion group. A year later, there was a significant difference in comparing the relapse rate between the two groups (P<0.05), revealing that the relapse rate was significantly lower in the acupuncture-moxibustion group than in the medication group.Conclusion Acupuncture-moxibustion can produce a marked therapeutic efficacy in treating EM.