Objective:This study is to explore available approaches to selling up a twin registry, which was based on their mental development and status of their mental health. Methods:In order to choose suitable approaches to setting up the twin registu, police, education committee-school and newspaper approaches are compared. Two-step-method is adopted to construct the twin registry. The first step is In collect basic data of twins and the second step is to contact children's guardians, get the signatures to the comprehending and consent document from the guardians and then bring the twins into the registry formally. Result:The successful recruit ratios of the police approach, the education commiltee-school approach and the newspaper approach are 4%, 42.6% and 29.2%. The refusing ratios of the education committee-school approach and the newspaper approach are 57.4% and 70.8%. Then we adopted a series of methods to reduce the refusing ratios. The refusing crowds were contacted again. The re-refusing ratio of the education committee-school approach and the newspaper approach are 31.03% and 55.88%. The overall refusing ratio of the education commillee-school approach and the newspaper approach are 17.82% and 39.58%. Conclusions:Every approach has its own advantages and disadvantages. The available approaches should be adopted to setting up the twin registry according to the characteristics of the research.

Objective: To investigate the chemical constituents of Abelmoschus esculentus. Methods: Compounds were isolated and purified by MCI, ODS, and silica gel column chromatography. Their chemical structures were elucidated on the basis of spectral data and physicochemical properties. Results: Twelve compounds were isolated and identified as tryptophan (1), deoxyguanosine (2), hypoxanthine (3), 3, 4-dihydroxy benzoate (4), 3′-deoxyinosine (5), thymidine (6), inosine (7), β-daucosterol (8), aurantiamide acetate (9), xanthine (10), ethyl-β-D-xyloside (11), and ethyl-α-D- arabinofuranoside (12). Conclusion: Except compound 1, other compounds are separated from A. esculentus for the first time.

Objective: To investigate the chemical constituents of Abelmoschus esculentusl. Methods: Compounds were isolated and purified by Sephadex LH-20, MCI, ODS, and silica gel column chromatography. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data ( 1H-NMR and 13C-NMR). Results: Twelve compounds were isolated and identified as: uracil (1), uridine (2), deoxyuridine (3), adenine (4), adenosine (5), 3′-deoxyadenosine (6), guanosine (7), deoxyguanosine (8), phenylalanine (9), tyrosine (10), leucine (11), and isoleucine (12). Conclusion: Compounds 1-8 are separated from A. esculentusl for the first time.

Objective To explore cardiovascular risk factors in patients of hypertension (HTN) complicated with non-alcoholic fatty liver but normal aminotransferase. Methods Forty-four cases (control group) of uncomplicated hypertension and 56 cases (study group) of hypertension complicated with nonalcoholic fatty liver but normal aminotransferase were screened from those who visited physical examination center of the hospital for regular checks-up during October 2009 to June 2010. Blood pressure ( BP), body mass index ( BMI), fasting plasma glucose (FPG), total serum cholesterol (TC) , triglyceride (TG), highdensity lipoprotein-cholesterol ( HDL-C ), low-density lipoprotein-cholesterol ( LDL-C ), gammaglutamyltransferase ( GGT ), uric acid ( UA ) , highly-sensitive C-reactive protein ( hs-CRP) and homocysteine (Hey) of the two groups were compared. Results Comparing with the control group, the study group had higher diastolic blood pressure ( DBP) (t = 1898. 5, P < 0. 05) , BMI (t = - 5. 036, P < 0.01), FPG (t= -2.026, P<0.05), TG (t = 1923. 5, P<0.05), GGT (t = 1789, P <0.01), UA 0 = 1715, P<0.01), hs-CRP (t = 1832.5, P < 0.01) and lower level of HDL-C (t =2357.0, P< 0. 01). Serum activity of GGT correlated positively with BMI (rs = 0. 349, P = 0. 000), TG ( rs = 0.413,P =0.000), UA (rs =0.446, P= 0.000) and hs-CRP (rs =0.350, P = 0.000), respectively.Conclusions When patients of hypertension complicated with non-alcoholic fatty liver but normal aminotransferase, their cardiovascular risk factors clustered further, with serum activity of GGT obviously increased which correlated positively with various risk factors, indicating increase in cardiovascular risks.

Objective To investigate whether serum miRNA-720 and miRNA-484 can be used as non-invasive biomarkers of colon cancer.Methods Real time-polymerase chain reaction was used to detect the expressions of miRNA-720 and miRNA-484 in serum from 104 colon cancer patients(divided intoⅠ~Ⅱ-stage group andⅢ~Ⅳ-stage group)compared with 60 ad-ditional healthy controls.ROC curve was performed to analyse the diagnostic usefulness of both miRNAs distinguishing be-tween different stages of colon cancer patients.Results Compared with healthy controls,the miRNA-720 was upregulated close to 2 times inⅠ~Ⅱ-stage group (t=1.997,P<0.05),and was upregulated about 3 times inⅢ~Ⅳ-stage group (t=2.133,P<0.05).The miRNA-484 was downregulated almost 2 times inⅠ~Ⅱ-stage group (t=2.585,P<0.05),but was upregulated over 3 times in Ⅲ~Ⅳ-stage group (t=3.416,P<0.01).The area under the ROC curve (AUC)for the diag-nosis of colon cancer were 0.76 (95%CI:0.67~0.86)and 0.79 (95%CI:0.69~0.89)respectively.The combined use of both miRNAs could make the AUC up to 0.87 (95%CI:0.77~0.96).Conclusion Serum miRNA-720 and miRNA-484 can be used as non-invasive biomarkers to diagnose early colon cancer.They can also distinguish between different stages of co-lon cancer patients.

Objective To study the effects of monosodium glutamate on viability and fertility of filial generation Drosophila.Methods Different concentrations(15,30,45 g/L)of L,H,T monosodium glutamate(MSG)were added in the medium for wildtype Drosophila melanogaster,and the non-MSG medium was taken as the control group,3 pairs of Drosophilas in each bottle and 3 repeats in each group,the first and second filial generation adult Drosophila's eclosion time(growth period) and the number of offspring within 7 days(reproduction quantity),and the death count in 30 days of 20 Drosophilas were observed and recorded.Results Compared with the control group,the reproduction quantity of Drosophila significantly increased,the growth period shortened in the first filial generation in 15 g/L of T MSG group.As the MSG concentrations increased,the reproduction quantity reduced significantly,the survival rate of Drosophila in 30 days declined significantly.The reproduction quantity in second filial generation increased at the initial stage of exposure and then decreased as the MSG concentrations increased.Conclusion The results of the present paper indicates that low-dose of MSG may be the advantage for survival and reproduction of Drosophila,but high doses will produce adverse effects.

Humans ; Integrative Medicine ; Myelodysplastic Syndromes ; therapy

Objective To establish a rapid, reliable method for identifying and typing of Clostridium botulinum using 16S rDNA sequencing and phylogenetic tree construction. Methods Clostridium genome templates were extracted from 9 strains, including LCL063, 830110, LC175, LCL155, 66418, N153, 61082, ALASKA, and IWAN AI; 16S rRNA genes were amplified by PCR with the 16S rRNA specific primers, and then the PCR products were cloned to pGEM-T Easy vector and sequenced. Finally, the sequence of 16S rDNA was analyzed by Clustal and Mega program; the Phylogenetic trees were constructed by Neighor joining and Maximum parsimony. Results It was found that LCL063, 830110, and LCL175 were BONT/E producing Clostridium butyricums; IWANAI and ALASKA were Clostridium botulccum type E. The results of the present method were consistent with those of the conventional method. Conclusion 16S rRNA sequencing combined with phylogenetic tree analysis a rapid and accurate method in Clostriduum bouulccum identification, and the method may serve as a criterion for bacterial typing with the completion of ribosomal RNA data bank.

Objective: To investigate the chemical constituents in the roots of Angelica dahurica var. formosana. Methods: The dried rhizomes of Angelica dahurica var. formosana were chopped and extracted with 80% EtOH for 3 times under reflux. The EtOH extract was isolated by chromatography on silica gel using a gradient solvent system (PE: EtOAc & CHCl3: MeOH) and Sephadex LH-20. The structures of the contents were elucidated by nuclear magnetic resonance and MS spectral analysis. Results: Seventeen compounds were identified, namely, isoimperatorin (1), imperatorin (2), bergapten (3), alloimperatorin(4), xanthotoxol (5), cnidilin (6), byakangelicin (7), bergaptol (8), byakangelicol (9), pabulenol (10), oxypeucedanin hydrate(11), desmodimine (12), palmitic acid (13), stiamasterol (14), β-sitosterol (15), β-daucosterin (16), and sucrose(17). Conclusion: Compounds 8 and 12 have been isolated from Angelica dahurica var. formosana for the first time. Compound 12 has been firstly isolated from Umbelifera plants. All the courmarin compounds belong to linear 6, 7-furanocourmarins.

Objective To study the chemical composition from the seeds of Annona squamosa. Methods The components of annonaceous acetogenins from the seeds of A. squamosa were isolated and purified by extracting and chromatography separation. The structures of the compounds were identified by their physicochemical properties, UV, NMR, and mass spectrometry data. Results Seven annonaceous acetogenins were isolated from the ethyl acetate extract of the seeds from A.squamosa. They are 3-[7-[5- [1,4-dihydroxy-4-[5-(1-hydroxytridecyl)-2-furanyl] butyl] tetrahydro-2-furanyl]-2-hydroxyheptyl]-5-methyl-2(5H)-furanone (1), 3-[9-[5-(1-hydroxy-4-heptadeceneyl) tetrahydro-2-furanyl] hydroxynonyl]-5-methyl-2 (5H)-furanone (2), bullatalicin (3), squamostatin A (4), squamostatin D (5), bullatacin (6), and 10-hydroxyasimicin (7). Conclusion Compounds 1 and 2 are two new compounds, named as trilobalicin I (1) and D20-solamin B (2), respectively.