Objective:Human metapneumovirus (hMPV),initially described in 2001,is an enveloped RNA virus of the genus Metapneumovirus,subfamily Pneumovirinae,family Paramyxoviridae. Here we sought to clarify basic features of human metapneumovirus proteins. Methods:Rabbits were immunized with inactivated virons of hMPV Chinese first isolate,CHN05-01,to yield anti-hMPV antiserum. Antiserum was used as primary antibody to detect hMPV proteins by Western blotting. NetNglyc1.0 server,NetOglyc 3.1server and the NetPhos 2.0 server were applied for predicting potential glycosylation and phosphorylation sites of proteins of prototype virus of type A,CAN97-83. Results:The highest reactive titer of the antiserum with hMPV antigens reached 1:500 in ELISA. Potential glycosylation sites of G protein and phosphorylation sites of P protein were greatest among all hMPV proteins. G protein was shown a narrow band with molecular weight between 55 and 72kDa (approximately 68kDa),indicating its glycosylation level being consistent and remarkably different from that of CAN99-80 and CAN99-81. F1 subunit of fusion protein displayed molecular weight between 40 and 55kDa (approximately 48 kDa),which is consistent with previous reports. Conclusion:Basic features of two major membrane proteins of Chinese human metapneumovirus isolate were clarified,which will benefit future studies on protein funtion and pathogenesis of this virus.

Objective To investigate the CT and MRI manifestations and clinical features of liver fluke granuloma.Methods A retrospective analysis was performed for the clinical and imaging data of 5 patients with pathologically confirmed liver fluke granuloma who were hospitalized in The First Affiliated Hospital of Guangxi Medical University from January 2010 to September 2015.Results Liver fluke granuloma had slightly low density on CT plain scan,as well as a slightly low signal on T1 weighted images and a slightly higher signal on T2 weighted images of MRI plain scan.Three-phase contrast-enhanced CT scan showed delayed enhancement with mild dilatation of the intrahepatic bile duct,and normal vessels ran through the lesion.Conclusion Liver fluke granuloma is a rare disease in chnical practice.A history of eating raw fish,delayed enhancement on three-phase contrast-enhanced CT scan,and normal vessels running through the lesion all contribute to the diagnosis of liver fluke granulomas.

Objective To investigate the clinicopathological characteristics and treatment of nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL). Methods Seven patients were confirmed as NLPHL by pathologic immunohistochemistry. Six patients received combined-modality therapy of chemotherapy and involved field, and the other one received single chemotherapy. Results The 3-year local control rate and overall survival rate were 100 % and 86 %, respectively. Only one case died of pulmonary infection following chemotherapy. Conclusion These patients with NLPHL has favorable prognosis, tolerance and less toxicity for combined-modality therapy. However the management of toxicity following treatment should be noted.

Feature extraction is a very crucial step in P300-based brain-computer interface (BCI) and independent component analysis (ICA) is a suitable P300 feature extraction method. But at present the convergence performance of the general ICA iteration methods are not very satisfactory. In this paper, a method based on quantum particle swarm optimizer (QPSO) algorithm and ICA technique is put forward for P300 extraction. In this method, quantum computing is used to impel ICA iteration to globally converge faster. It achieved the purpose of extracting P300 rapidly and efficiently. The method was tested on two public datasets of BCI Competition II and III, and a simple linear classifier was employed to classify the extracted P300 features. The recognition accuracy reached 94.4% with 15 times averaged. The results showed that the proposed method could extract P300 rapidly and the extraction effect did not reduce. It provides an experimental basis for further study of real-time BCI system.
Algorithms ; Brain-Computer Interfaces ; Electroencephalography ; Event-Related Potentials, P300 ; Humans ; Principal Component Analysis ; Signal Processing, Computer-Assisted

OBJECTIVE:To reference clinical utilization of amikacin of a hospital.METHODS:By retrospective survey,the clinical information of 30 inpatients who were treated with amikacin and received plasma concentration monitoring from Apr.2006 to Feb.2010 were analyzed statistically in respect of primary disease,results of pathogen culture and drug sensitivity test,dosages and usage,plasma concentration,clinical efficacy,adverse drug reactions,etc.RESULTS:Clinical response rate was 55.2% and dosage of amikacin was small as well as measured value and reference value of plasma concentration.No adverse drug reaction was observed.CONCLUSION:The proportion of amikacin should be increased and plasma concentration monitoring of amikacin should be strengthened.Individual regimen is realized to improve effectiveness and safety of drug use.

Objective To explore the causes of the special cases showing the EKG changes of cardiac infarction. Methods To analyse the causes of 30 special cases showing the EKG changes of cardiac infarction between 2000 and 2004. Results Among 30 special cases there were 11 cases of viral myocarditis( 36. 7 % ), 7 cases of acute pancreatitis(23.3% ) ,6 cases of hypertrophic obstructive cardiomyopathy(20% ) ,4 cases of bile-cyst and heart syn-drome(13. 3% ) and 2 cases of pulmonary embolism(6. 7% ). Conclusions There are many causes showing the EKG changes of cardiac infarction. It isn't the typical symptom of the cardiac infarction. It should be combined with the clinic and be distinguished timely and exactly in order to avoid the misdiagnosis.

corrected visual acuity and good visual quality are clinic effects standard of laser in situ keratomeleusis.Visual quality descending for patients after LASIK surgery depends on many factors.This review recommends analyses the influence of visual quality for patients after LASIK surgery and it's guiding significance to LASIK from seven sides,such as visual acuity,refactive diopter,contrast sensitivity,wavefront aberration,increment of Q-value,binocular visual function,the change of dominant eye.

1 case of intraductal papilloma of parotid gland was analyzed by means of clinicopathologic data,hematoxylin-eosin and immuno-histochemical staining.Combined with the relevant literature,clinical pathological features,diagnosis and treatment of the salivary gland intra-ductal papilloma were discussed.

We use chitosan and n -acetyl -d -glucosamine to inhibit fibroblast proliferation which was cultured in vitro. The result shows that n - acetyl - d - glucosamine: ID50=16. 35mg/L, chitosan: ID50=69. 59mg/L. We also plant the chitosan membrane to the rabbit body to test its bio-degradation. The result shows that the molecular weight of chitosan drops 17% and 6% after 25 days and 18 days, respectively. On the basis of these results, we have obtained a conclusion that we can probably use chitosan to continuously release n-acetyl -d-glucosamine which can inhibit fibroblast proliferation effectively.

Objective:To study the antigenicity of OMP extracted from Edwardsiella tarda.Methods:ELISA, Bactericidal test, Agglutinating test and Western blotting were used for testing the antigenic titers and immunogenicity of OMP.Results:In immunoblotting, by using ATCC15947 OMP antibody, the non pathgenic strains were negative, while all pathogenic strains except Et 122 gave positive results and had OMP bands of 33k, 35k, 38k, and 45k. OMPs of both ATCC 15947 and JEL4 could induce high antibody titers. Further more, the antibodies evoked by OMPs of ATCC 15947 of 33k or 35k could also protected mice to some degree when diluted.Conclusion:The 33k, 35k, 38k, and 45k of OMPs may be protective antigens, and the OMPs of Et could be a candidative component for vaccine.