Objective:Human metapneumovirus (hMPV),initially described in 2001,is an enveloped RNA virus of the genus Metapneumovirus,subfamily Pneumovirinae,family Paramyxoviridae. Here we sought to clarify basic features of human metapneumovirus proteins. Methods:Rabbits were immunized with inactivated virons of hMPV Chinese first isolate,CHN05-01,to yield anti-hMPV antiserum. Antiserum was used as primary antibody to detect hMPV proteins by Western blotting. NetNglyc1.0 server,NetOglyc 3.1server and the NetPhos 2.0 server were applied for predicting potential glycosylation and phosphorylation sites of proteins of prototype virus of type A,CAN97-83. Results:The highest reactive titer of the antiserum with hMPV antigens reached 1:500 in ELISA. Potential glycosylation sites of G protein and phosphorylation sites of P protein were greatest among all hMPV proteins. G protein was shown a narrow band with molecular weight between 55 and 72kDa (approximately 68kDa),indicating its glycosylation level being consistent and remarkably different from that of CAN99-80 and CAN99-81. F1 subunit of fusion protein displayed molecular weight between 40 and 55kDa (approximately 48 kDa),which is consistent with previous reports. Conclusion:Basic features of two major membrane proteins of Chinese human metapneumovirus isolate were clarified,which will benefit future studies on protein funtion and pathogenesis of this virus.

Objective To investigate the CT and MRI manifestations and clinical features of liver fluke granuloma.Methods A retrospective analysis was performed for the clinical and imaging data of 5 patients with pathologically confirmed liver fluke granuloma who were hospitalized in The First Affiliated Hospital of Guangxi Medical University from January 2010 to September 2015.Results Liver fluke granuloma had slightly low density on CT plain scan,as well as a slightly low signal on T1 weighted images and a slightly higher signal on T2 weighted images of MRI plain scan.Three-phase contrast-enhanced CT scan showed delayed enhancement with mild dilatation of the intrahepatic bile duct,and normal vessels ran through the lesion.Conclusion Liver fluke granuloma is a rare disease in chnical practice.A history of eating raw fish,delayed enhancement on three-phase contrast-enhanced CT scan,and normal vessels running through the lesion all contribute to the diagnosis of liver fluke granulomas.

Objective To investigate the clinicopathological characteristics and treatment of nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL). Methods Seven patients were confirmed as NLPHL by pathologic immunohistochemistry. Six patients received combined-modality therapy of chemotherapy and involved field, and the other one received single chemotherapy. Results The 3-year local control rate and overall survival rate were 100 % and 86 %, respectively. Only one case died of pulmonary infection following chemotherapy. Conclusion These patients with NLPHL has favorable prognosis, tolerance and less toxicity for combined-modality therapy. However the management of toxicity following treatment should be noted.

The paper sorts out and analyzes papers on medical English teaching and research published in core periodicals of China. The results show that there are various problems: slow research progress, insufficient empirical studies, a shortage of research on teachers and students, a lack of young faculty members and insufficient international perspective. With the increasing requirement for medical students' higher language proficiency imposed by the development of higher medical education in China, this paper puts forward some suggestions based on the analysis of the past ten-year data on medical English teaching and research: to build stronger medical English teaching faculty and to enhance their research competence by means of improving, enlarging and cooperating; to intensify the research into the two subjects in the teaching process: medical English teachers and medical students; and to avoid depending too heavily on questionnaire survey and employ multiple research methods and collect effective data in order to fundamentally improve medical English teaching and research.

Objective:To study the antigenicity of OMP extracted from Edwardsiella tarda.Methods:ELISA, Bactericidal test, Agglutinating test and Western blotting were used for testing the antigenic titers and immunogenicity of OMP.Results:In immunoblotting, by using ATCC15947 OMP antibody, the non pathgenic strains were negative, while all pathogenic strains except Et 122 gave positive results and had OMP bands of 33k, 35k, 38k, and 45k. OMPs of both ATCC 15947 and JEL4 could induce high antibody titers. Further more, the antibodies evoked by OMPs of ATCC 15947 of 33k or 35k could also protected mice to some degree when diluted.Conclusion:The 33k, 35k, 38k, and 45k of OMPs may be protective antigens, and the OMPs of Et could be a candidative component for vaccine.

OBJECTIVE:To investigate the effects of Danhong injection combined with low molecular weight heparin (LM-WH) on clinical symptoms,serum inflammatory factors and lung function in elderly patients with chronic obstructive pulmonary disease (COPD). METHODS:A total of 146 elderly patients with COPD were randomly divided into observation group and con-trol group,with 73 cases in each group. Both groups received conventional treatment. On this basis,control group was given Dan-hong injection 30 mL,intravenous drip,once a day. Observation group was additionally given LMWH sodium injection 5000 IU, subcutaneously,twice a day,on the basis of control group. Both groups were treated for 14 d. Clinical efficacies of 2 groups were observed and compared. The clinical symptom score,the levels of IL-6,IL-8,TNF-α,FEV1,FEV1/FVC,pa(O2),pa(CO2) and the occurrence of ADR were compared before and after treatment. RESULTS:After treatment,the total response rate of observa-tion group was significantly higher than that of control group(93.15%vs. 78.08%,P<0.05). Before treatment,there was no statis-tical significance between above indexes (P>0.05). After treatment,individual clinical symptom score,total clinical symptom score,the levels of IL-6,IL-8,TNF-α,pa(CO2)in 2 groups were significantly lower than before;the observation group was signif-icantly lower than the control group;FEV1,FEVl/FVC,pa(O2) were significantly higher than before,and the observation group was significantly higher than the control group,with statistical significance (P<0.05). CONCLUSIONS:Based on conventional treatment,Danhong injection combined with LMWH is effective in the treatment of senile COPD,and can significantly improve the clinical symptoms,serum inflammatory factors levels and pulmonary function with good safety.

Objective To investigate the clinical efficacy of gemcitabine in the treatment of elderly patients with advanced non-small cell lung cancer.Methods 50 patients in our hospital from January 2014 to September 2015 were selected as the research object, and were randomly divided into the control group and the experimental group, each group of 25 patients each.The patients in the control group were treated with special therapy, and the patients in the experimental group were treated with gemcitabine.The clinical indexes of the experimental group and the control group were compared and analyzed.Results After the treatment, the experimental group of 25 patients, 12 cases of patients with complete remission, 8 cases partial remission, 3 cases were stable, 2 patients progressed, the effective rate was 75%, clinical benefit rate of 92%.In the control group, 4 patients had complete remission, 2 patients had partial remission, 9 patients were stable, 10 patients progressed, the treatment efficiency was 24%, and the clinical benefit rate was 60%.The effective rate and the clinical benefit rate of the experimental group were significantly higher than that of the control group (P<0.05).1 years later, the survival rate of patients in the experimental group was 22, the survival rate was 88%, and the number of patients in the control group was 14.The survival rate of the experimental group was significantly higher than that of the control group, with statistical difference (P<0.05).Conclusion Gemcitabine monotherapy in the treatment of elderly patients with advanced non-small cell lung cancer can improve the therapeutic effect to a great extent, good safety, low incidence of adverse reactions, high survival rate, with further clinical promotion and application significance.

Objective To investigate the differences in the clinical application of the etiological subtypes of ischemic stroke according to the TOAST,ASCO and CISS criteria.Methods A total of 167 consecutive patients with fist-ever ischemic stroke were enrolled in the study.They were classified with the TOAST,ASCO and CISS criteria,respectively.The differences and consistency of subtype diagnosis of each etiology were compared.Results Compared with TOAST,ASCO grade 1 significantly increased the proportion of patients in a large atherosclerosis group (23.4％ vs.19.8％ ;x2 =4.167,P =0.031) and decreased that in a small vessel disease group (32.9％ vs.38.3％ ; x2 =4.923,P =0.022); similarly,CISS also significantly increased the proportion of patients in the large atherosclerosis group (37.1％ vs.19.8％ ;x2 =27.034,P ＜0.001)and decreased that in the small vessel disease group (19.2％ vs.38.3％;x2=25.289,P ＜ 0.001).However,ASCO grade 1 (34.1％ vs.28.1％;x2 =3.682,P=0.052) and CISS (32.9％ vs.28.1％;x2 =0.880,P =0.268) did not decrease the proportion of patients in a cryptogenic group.The consistency of the 3 typing methods was between moderate (other etiology group of TOAST/ASCO-1,κ =0.434) and excellent (cardioembolism group of TOAST/ASCO-1,κ =0.967).Conclusions Both ASCO-1 or CISS typing may not decrease the proportion of the patients with the subtype of cryptogenic stroke,but the consistency among all the subtypes was better.The design and characteristics of the diagnostic criteria in all subtypes with the 3 criteria should be considered in clinical practice.

Objective: To establish a method for the determination of ketoconazole and miconazole nitrate in compound ketocon-azole gels. Methods:An HPLC method was developed. A Hypersil BDS C18 column(416 mm × 200 mm,5μm) was used, the mobile phases consisted of 0. 5% ammonium acetate solution-methanol(containing 0. 2% triethanolamine) (20∶80), the flow rate was 1. 0 ml ·min-1 , the detection wavelength was set at 230nm, the column temperature was 30℃ and the injection volume was 20μl. Results:There was a good linear correlation within the range of 5. 1-510. 0 mg·L-1 for ketoconazole (r=0. 999 9) and 50. 0-500. 0 mg·L-1 for miconazole nitrate (r=0. 999 9), the average recovery for ketoconazole and miconazole nitrate was 100. 3%(RSD=0. 38%, n=6) and 99. 9%(RSD=0. 79%, n=6), respectively. Conclusion:The method is simple, rapid, accurate and sensitive, and can pro-vide a method for controlling the quality of compound ketoconazole gels.

Etiologic classification of ischemic stroke is the basis for epidemiological investigation, risk factor screening developing individualized treatment plan and prognostic measures. The etiologic classifications, such as TOAST, CCS, and ASCO, are more generally accepted methods at present. This article reviews the advantages and disadvantages from the diagnostic criteria and clinical application.