Humans ; Lead ; Spectrometry, Fluorescence ; Spectrophotometry, Atomic ; Uncertainty

Se load tests were carried out by administering 150?g Se as 0.1% sodium selenite 0. 5ml in 5 groups of male children (7～13 years) including 2 groups from different Kashin-Beck disease affected areas, 2 groups from non-endemic areas and another from an endemic area supplementing Se-fortilled salt for 4 years. The results showed that Se urinary excretion amounts, 6,12 and 24 hour accumulated rates and 24 hour net excretion amounts as well as rates after Se administration were similar between 2 endemic groups, but all far lower than those of 2 non-endemic groups; that urinary Se excretion peak in endemic groups delayed as comparied with non-endemic groups and that the above—mentioned parameters in endemic group supplementing Se restored to the levels of non-endemic groups. All those findings suggested that the children from Kashin-Beck disease affected areas might be in a Se deficiency status and Se supplementation to the endemic children consuming Se fortified salt significantly improve their Se deficiency status.

Objective To explore the expression of Hes1 mRNA during neural stem cells(NSC) differentiation toward neurons . Methods To establish the model of cultivation NSC in the hippocampal of newborn (24 h) SD rats ,and then to observe the mor-phology of NSC in the course of proliferation and differentiation .Before and after cellular induction ,the expression of Nestin and NSE were respectively measured to detect cell types by immunochemistry method .And flow cytometry was used to determine cell cycle phases ,so as to detect proliferative activity of these cells .Meanwhile ,the expression of Hes1 mRNA in NSC was determined by reverse transcription-PCR(RT-PCR) .Results The results demonstrated that NSC isolated from hippocampal showed vigorously clonal proliferation in vitro ,and positive Nestin expression .In addition ,the differentiated cells demonstrated positive NSE expres-sion .Flow cytometry analysis showed that the percentage of NSC in S phase was obviously higher than that of induced differentia-tion of all time(P<0 .01) ,which indicated that NSC were actively dividing induction before .Compared to NSC ,the percentages of cells in G0 G1 phases were increased significantly after neuronal differentiation (P<0 .01) ,which indicated that differented cells have arrested in G0 G1 phases .Meanwhile ,the results from RT-PCR showed that :Hes1 mRNA was expressed in NSC both before and af-ter induced differention .Compared to induction before ,the level of Hes1 mRNA expression in NSC during different stages of differ-entiation after induction were significantly decreased (P<0 .05) ,and Hes1 mRNA did not show any obvious changes among these stages of differentiation(P>0 .05) .Conclusion The high level of Hes1 mRNA was probably involved proliferation of NSC .How-ever ,low level of Hes1 mRNA might contribute to neuronal differentiation .

ObjectiveTo investigate the action mechanism of Th17 cells in immunoinflammatory response in systemic lupus erythematosus(SLE).MethodsReverse-transcription PCR was performed to measure the mRNA expre ssion of the retinoic acid receptor-related orphan nuclear receptor RORγt in peripheral blood mononuclear cells (PBMCs) from 12 patients with active SLE,9 patients with inactive SLE and 12 normal human controls.Data were statistically analyzed by approximate F test(Welch test) and Dunnett's T3 multiple comparison test (corrected).ResultsIn the case of RORγt mRNA expression in PBMCs,significant differences existed among the 3 groups(F =23.286,P ＜ 0.01 ); in detail,the patients with active SLE were significantly higher than patients with inactive SLE and normal controls ( 1.06 ± 0.44 vs.0.65 ± 0.25,F =2.453,P ＜ 0.05;1.06 ± 0.44 vs.0.22 ± 0.08,F =6.504,P ＜ 0.05),and the patients with inactive SLE were significantly increased compared with the normal controls (F =3.343,P ＜ 0.05).The expression level of RORγt mRN A was significantly positively correlated with SLE disease activity index (rp =0.623,P ＜ 0.01 ).ConclusionsThere is a polarization of Th17 cells in patients with SLE.To antagonize the transcription factor RORγt,which plays an essential role in the regulation of Th17 cell differentiation,may facilitate the control of SLE via attenuating the immunoinflammatory response.

Objective: To investigate the expression of Wnt/β-catenin pathway in diabetic nephropathy (DN) rats and the intervention effect of Chinese materia medica (CMM) for dispersing blood stasis and dredging collateral. Methods: Ten rats were selected as control group from 60 rats, the remaining rats were established as DN models by feeding high glucose and high fat diet combined with low-dose streptozotocin ip injection. Model rats were randomly divided into model group, irbesartan treatment group, and CMM group. The rats in each group were ig administered with corresponding drug, at the end of the 20th week, the 24 h urinary total protein was detected. The expression levels of Wnt4 and β-catenin mRNA and protein in renal tissue were detected. Results: Compared with control group, the 24 h urinary total protein, expression of Wnt4, β-catenin mRNA, and protein significantly increased in the model group (P < 0.01). Compared with model group, 24 h urinary total protein, the expression of Wnt4, β-catenin mRNA, and protein decreased significantly in irbesartan group and CMM group (P < 0.01 or P < 0.05). Conclusion: CMM for dispersing blood stasis and dredging collateral might decrease proteinuria in DN rats. It can also inhibit the high expression of Wnt/β-catenin pathway in the kidney of diabetic nephropathy rats. The effect might be one of the main ways to reduce urinary protein excretion.

ObjectiveTo explore the effective methods to improve the pulmonary function and life quality of patients with idiopathic pulmonary fibrosis (IPF).Methods48 IPF patients were randomly divided into two groups, each group includes 24 cases. Group A was treated with prednisone 0.5 mg/kg per day orally, reduce half of the dosage 4 weeks later and Fluimucil 600 mg three times per day orally, while group B with prednisone 0.5 mg/kg per day orally, reduce half of the dosage 4 weeks later. The clinical manifestation, pulmonary function testing, high-resolution CT of the chest, arterial blood gas analysis were observed before and 3 months after treatment. ResultsThere was significant difference in Group A (P<0.05) but no significant difference in Group B (P>0.05) before and after treatment in the pulmonary function testing and arterial blood gas analysis. There was significant difference after treatment in the pulmonary function testing and arterial blood gas analysis between Group A and Group B (P<0.05).There was significant difference after treatment in the clinical manifestation, high-resolution CT of the chest and between Group A and Group B (P<0.05). ConclusionHigh dose Fluimucil can ameliorate the pulmonary function, relieve the symptom of short breath of patients with idiopathic pulmonary fibrosis.

Viral myocarditis (VMC) is a clinical frequent cardiovascular disease with extremely high mortality and disability rate ,which seriously impairs the health of children and young adults ,and its pathogenesis has not been fully elucidated .It is generally believed that VMC pathogenesis is mainly related to direct damage on myocardial cells ,immune damage ,cell apoptosis and cytokines .The present article made a summary about the research progress of its pathogenesis .

Mesial temporal lobe epilepsy (mTLE) is the most common type of refractory focal epilepsy. Diffusion tensor imaging (DTI) can provide qualitative and quantitative information on the microstructural arrangement of epileptic brain tissue. DTI is significant for insights to the pathophysiological mechanism of mTLE, and has potential for investigation of changes in structural connectivity of mTLE. The development and application of DTI in research of mTLE were reviewed in this article.

Objective To assess the effectiveness of the acupuncture treatmeat on anxiety compared with non-acupuncture intervention. Method All of the randomized or quasi-randomized trials involving were reviewed systemantically with the Meta analysis method. The homogeneity test was mode, the standandised mean difference (95% CI) and add risk (OR, 95% CI) were tested. Results Homogeneity test was made among the trials and no significant difference between the acupucture and non-acupuncture groups. Fixed effect model was used. ORP =1.759 419 579, 95% CI (1.336 391, 2.316 356), there was significant difference between the acupuncture group and medicine group as the interventions used to treat anxiety while there was no difference between the two group on the influence in HAMA. Conclusion The Meta analysis results was a trend in favor of acupuncture effectiveness. It seems no serious adverse reactions have been found. But there was no sufficient reliable evidence due to the low quality of the trials and possible publication bias. Further randomized, double blind controlled trials are needed.

Objective To observe the clinical effect of continuous intravenous pumping of octreotide in the treatment of intestinal endotoxemia.Methods Eighty patients with intestinal obstruction and non-surgical treatment were divided into group Ⅰ with 34 cases who received conventional-treatment and group Ⅱ with 46 cases who received conventional-treatment combined with octreotide 24 h continuous intravenous pumping.White blood cell count ( WBC ),diamine oxidase (DAO),D-lactic acid (D-LA) and endotoxin were detected before treatment and at 24 h,48 h,4 d after treatment.Results The content of WBC,DAO,D-LA and endotoxin in two groups all reached peak at 48 h after treatment.The difference of the content of WBC,DAO,D-LA and endotoxin between two groups had no statistical significance at 24 h after treatment (P ＞ 0.05).The content of WBC,DAO,D-LA and endotoxin of group Ⅱ at 48 h and 4 d after treatment were lower than those of group Ⅰ.And the difference at 48 h after treatment had statistical significance[(18.40 ±0.10)× 109/L vs.(20.60 ± 2.36) × 109/L,(6.12 ± 1.02) kU/L vs.(8.02 ± 1.54) kU/L,(2.14 ±0.21) mg/L vs.(3.34 ± 0.04) mg/L,(1.65 ±0.16) kEU/L w.(2.23 ±0.36) kEU/L] (P ＜ 0.01).While the difference at 4 d after treatment had no statistical significance(P＞ 0.05 ).Body temperature at 48 h after treatment,gastrointestinal decompression capacity,anus exhaust time of group Ⅱ were (37.60 + 3.01 )℃,(320.00 ± 76.14) ml/d,(54.00 ± 0.94) h respectively,and they all were superior to those of group Ⅰ[(38.50 ± 2.21 ) ℃,(500.00 ± 80.32) ml/d,(68.00 ± 1.02) h] (P ＜0.01).Conclusions Continuous intravenous pumping of octreotide can effectively protect the intestinal mucosal barrier function,improve intestinal permeability,reduce the trmslocation of intestinal flora,inhibit the incidence and development of enterogenous endotoxemia.And it provides new evidence to support the clinioal application of octreotide in patients with intestinal endotoxemia.