Objective To prepare and characterize glycyrrhetinic acid cationic liposomes.Methods Liposomes were prepared by ethanol injection technique.An orthogonal test was utilized to optimize the formulation and preparation of glycyrrhetinic acid liposomes.The unencapsulated glycyrrhetinic acid and liposomes were separated by Sephadex gel G-50,the encapsulation efficiency was detected by HPLC.The morphological examination of glycyrrhetinic acid liposomes was performed using transmission electron microscopy.The particle size and Zeta potential of the liposomes were measured.The release rate of glycyrrhetinic acid from liposomes was tested.Results The liposomes with spherical or ellipsoidal shape and better stability featured the encapsulation efficiency of(91.61?1.16)%,the mean partical size of(141?10)nm,and Zeta potential of(35.9?5)mV.The in vitro release kinetics were consistent with Higuchi equation.The stability of glycyrrhetinic acid liposomes was better.Conclusion The selected formulation and preparation technic of glycyrrhetinic acid liposomes could be rational,stable,and with a sustained feature in vitro release.

Objective To determine the encapsulation efficiency of glycyrrhetinic acid(GA) liposomes and to study the release of GA from the liposomes in vitro.Methods GA liposomes were prepared by ethanol injection.The unencapsulated glycyrrhetinic acid and liposomes were separated by sephadex gel G-50,and the encapsulation efficiency was detected by HPLC.The release behavior of the drug was studied by the third method for dissolution issued in Chinese pharmacopoeia(published in 2005).Results The encapsulation efficiency of liposomes was 91.61%,and the in-vitro drug-release curve was accorded with Higuchi equation.Conclusion The GA liposomes have high encapsulation efficiency and sustained-release effect.

【Objective】 To observe the therapeutic effect of focus-locating scalp circum-needling for ischemic stroke (IS) at acute stage. 【Methods】 Sixty-one cases of IS were randomized to two groups: group A (n=31) was treated with acupuncture on homolateral scalp region projected by CT-located focus; group B (n = 30) was treated with acupuncture on conventional scalp regions which were in charge of motor and sensory function. The cases were treated with acupuncture once a day for 30 days. The changes of symptoms and signs and the plasma contents of norepinephrine (NE) and epinephrine (E) were observed before and after treatment. 【Results】 Twenty cases were cured, 10 markedly effective, 1 effective and 0 ineffective in group A; 11 cases were cured, 15 markedly effective, 4 effective and 0 ineffective in group B. The therapeutic effect in group A was better than that in group B (P