BACKGROUND:The narrowing of intervertebral space induced by the intervertebral disc degeneration is mainly characterized by the expression of proteoglycan in nucleus pulposus cells and the reduction of col agen type II. OBJECTIVE:To quantitatively observe col agen type II protein in adult normal and degenerative intervertebral disc nucleus pulposus cells by immunofluorescence staining and safranin O staining.
METHODS:The nucleus pulposus specimens were col ected from adult scoliosis patients and patients with intervertebral disc protrusion, who were al volunteers. After culture, 26 cells in each patient were measured. There were 78 cells in both normal group and degeneration group. The normal and degenerative intervertebral disc nucleus pulposus cells were subjected to safranin“O”staining, and gray values were determined;intracellular col agen type II was detected by immunofluorescence staining.
RESULTS AND CONCLUSION:Immunofluorescence staining revealed that, degenerative intervertebral disc nucleus pulposus cells were only mildly stained, with the fuzzy staining, the shape was round, spindle, fusiform and irregular. There were a very smal amount of fluorescent particles within cells. The expression of col agen type II was decreased significantly compared with normal cells (P<0.05). Safranin O staining showed that, degenerative nucleus pulposus cells began to swel , the nuclei swel ed and were stained slightly, cellprocesses were prolonged, cytoplasmic dyeing was uneven accompanying with vacuole, celldisruption, scattered and chaotic distribution were visible, patches of necrosis were observed. The image gray value showed no significant difference compared with normal nucleus pulposus cells (P>0.05). The degenerative intervertebral disc nucleus pulposus cells have a smal quantity and partial y become apoptotic, the content of col egen type II protein is decreased significantly compared with normal nucleus pulposus cells.

0.05). But Xuezhikang was superior to Pulagu in decreasing TG level and raising HDL C level ( P

Objective To study the relationship between glycemic control and platelet parameters in newly diagnosed type 2 diabetic patients. Methods Ninety patients newly diagnosed as type 2 diabetes and 90 healthy people were enrolled into the study. Their blood pressure, platelet parameters, including blood platelets count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), fasting blood glucose (FPG),HbA1c ,triglyceride ( TG ), total cholesterol ( TC ), high density lipoprotein cholesterol (HDL-C ), low density lipoprotein cholesterol(LDL-C) were examined and the data were analyzed. Results The level of FBG, TG, TC,MPV and PDW of patients newly diagnosed as type 2 diabetic were significantly higher than those of healthy people (t =6. 405,2. 069,2. 633,2. 178,2. 103 ;P =0. 001,0. 046,0. 010,0. 031 and 0. 043 respectively); The level of FBG, HbA1c, TC, TG, MPV and PDW in the diabetic patients, with their HbA1 c ＞ 7%, were significantly higher than the patients with HbA1c ≤ 7 % ( t = 5.691,2.013,2. 345,3. 467,4. 016,2. 893, P = 0. 001, 0. 038,0. 029,0. 013, 0. 004, 0. 026 ). There were positive correlations between MPV and HbA1c ( r = 0. 389 P =0. 020), MPV and PDW ( r = 0. 324 P = 0. 01 ) in diabetic patients, but no correlation between MPV and FBG,MPV and Systolic BP or MPV and Diastolic BP (Ps ＞ 0. 05 ). The level of FBG, HbA1c, TG, MPV and PDW decreased significantly in the diabetic patients with HbA1c ＞ 7% after treatment (t = 5. 591,2. 301,2. 410,2.204,2.105; P=0.001,0.031,0.023,0.035,0.041, respectively).Conclusion The platelet activity enhanced in newly diagnosed type 2 diabetic patients, platelet activity can be recovered through glycemic control,which may prevent the role of platelet in diabetes complications in these patients.

BackgroundDomestic researches on the professional identity of psychiatrists are still relatively rare up to now， and effective evaluation tools remain inadequate. ObjectiveTo construct a professional identity questionnaire for psychiatrists with high reliability and validity， so as to provide an effective tool for the evaluation of professional identity of psychiatrists. MethodsA self-designed questionnaire titled "Professional Identity Questionnaire" was compiled. After preliminary screening of items and expert consultation， an initial questionnaire was formed and administered to a sample of 400 psychiatrists， and the final questionnaire was assessed by item analysis， reliability analysis and validity analysis. ResultsThe Cronbach's α coefficient was 0.930 for the final questionnaire， and ranged from 0.817 to 0.920 for each dimension. The value of KMO was 0.904 （χ²=2 931.652， P<0.01）. The cumulative variance contribution rate of factors extracted from exploratory factor analysis was 68.306%. Confirmatory factor analysis showed that the integrity of fit indexes were χ²/df=1.234， GFI=0.905， NFI=0.916， RMSEA=0.036， CFI=0.983， IFI=0.983. The final version of the professional identity questionnaire for psychiatrists consisted of 23 items relating to 4 dimensions， namely， professional cognition， professional behavior， professional emotion and professional values. ConclusionThe professional identity questionnaire for psychiatrists has good reliability and validity， and can be used as an effective tool to evaluate the professional identity of psychiatrists. ［Funded by Foundation of Sichuan Research Center of Applied Psychology of Chengdu Medical College （number， CSXL-22304）； Science and Technology Guiding Plan Project of Guangyuan City （number， 22ZDYF0072）］