Objective:To investigate the effect of TNBG on proliferation of human hepatocellular carcinoma cell lines SMMC-7721 and HepG2.Methods:MTT assay was used to test the effects of anti-proliferation.The flow cytometric analysis was used to detect the cell cycle distribution.Western blot was adopted for detecting the expression of cyclinB1 and p34 cdc2 .Results:Anti-proliferation effects were observed in SMMC-7721 and HepG2 after intervention of TNBG for 72 hours,and the effects were in concentration-dependent manners.The cells showed S and G2/M arrest in both of cell lines,and apoptosis was induced only in HepG2.The expression of cyclinB1 was inhibited,while p34 cdc2 was not changed.Conclusion:TNBG can inhibit the proliferation of tumor cells,which is due to G2/M arrest caused by expression inhibition of cyclinB1 induced by TNBG.

Objective:To analyze the postoperative recurrentive factors of oral squamous cell carcinoma.Methods: 673 patients of primary oral squamous cell carcinoma were involved. The focus location, growth type, T stage, lymphonodus metastasis, treatment, pathological grading and resection mode of mandible were evaluated to determine the prognosis of patients.Results:The postoperative recurrence rate was relevant to the focus location, growth type, T stage, lymphonodus metastasis and pathological grading, while the treatment and resection mode of mandible had no effects on the recurrence rate. Conclusion: It's of great importance to estimate all kinds of clinicopathologic factors and perform preventive treatments during the clinical work to promote the operative cure rate and survival rate of patients with oral squamous cell carcinoma.

Objective:To prepare an acellular matrix from trachea of rabbits and SD rats, and to investigate its biocompatibility.Methods: A modified detergent and enzyme link extraction procedure was performed to remove cells from the trachea of SD rats and rabbits. The histology, topography of inner-surface and biocompatibility were studied by morphological observation,cell culture and in vivo transplantation respectively.Results:The acellular trachea matrix did not inhibit the growth and amylase secretion of allogenic salivary gland cells cultured on it.The allogenically transplanted acellular trachea matrix did not result in inflammation reaction of the host tissue,could integrate with surrounding tisses.Conclusion:The acellular trachea matix is biocompatible.

Objective:To study the effects of isoproterenol(Iso) on the proliferation of cultured submandibular gland cells of SD rats. Methods:Submandibular gland cells of SD rats were primarily cultured. The cells were exposed to Iso at 10 -3 g/L continuously for 8 days or intermittently(2 h each day for 8 days). The cell proliferation was sutdied by bromodeoxyuridine Brdu labeling and cell counting.Results:Iso increased cell proliferation(P

s Objective: To study the feasibility of allogeneic tra ns plantation of myoblasts of SD rats for the reconstruction of facial muscle de fects. Methods: Myoblasts obtained from the forelimbs and hindlimbs of neonatal SD rats were purified and cultured. 5?10 4 myoblasts w ere seeded onto each of type Ⅰcollagen sponges in the size of 1.5 mm?0.8 mm? 0.5 cm. The myoblasts/sponge constructs were transplanted into the facial muscl e defects of syngeneic mutured rats and observed with microscopy,immunohistoche mistry and electromyography (EMG).Result:Myoblasts attac hed and kept alive on type I collagen for at lest 3～4 days in culture. 4 and 8 w eeks after transplantation,muscle like tissue with blood vessles was observed i n the implants. Expression of actin and myosin in the implants was similar to th at in the control muscle.8 weeks after implantation,the spike wave (mV) on the g rafted side and control side was 0.7?0.3 and 2.7?0.6 ( P

Objective: To explore the effect of immune reaction induced by alginate on parotid acinar cells in vitro. Methods: Rabbits were immunized from the conjugated alginate- BSA (1.0 mg/kg) by 40-days routine immunity method. ELJSA method was used to examine the titration (valence) of anti-alginate serum. Five groups (group A: contrast, group B: BSA, group C; alginate, group D: anti-alginate serum, group E; alginate + anti-alginate serum) were examined by MTT method at four time points( 1, 6,12 and 24 h). The growth and morphology of parotid acinar cells were observed under inverted phase contrast microscope and scanning electron microscope. Results: Antibody-serum was acquired by routine immunity method, and the titration (valence) of anti-alginate serum was 1: 400. MTT results showed that the proliferation of parotid acinar cells had been limited at 24 h( P <0.05), the other three time points showed no difference. Under inverted phase contrast microscope, a few of acinar cells whose membranes were destroyed after 12 h, some cell contents leaked out. The holes in membrane could be seen early at 6h under scanning electron microscope. Most of the acinar cells were broken at 24 h. Conclusion: The antibody-serum to alginate and immunized rabbit was acquired by routine immunity method. The immune reaction induced by alginate can destroy parotid acinar cells in vitro.

T1 mapping is a new magnetic resonance imaging technique .By which the longitudinal relaxation time constant ( T1) of the myocardium can be measured to detect and quantitatively assess various focal or diffuse diseases .T1 mapping has various scan sequences , such as LL sequence , MOLLI sequence and ShMOLLI sequence .T1 mapping can be classified into two groups:pre--contrast and post-contrast T1 mapping.In addition, by measuring post-contrast T1 mapping and pre-contrast T1 mapping, myocardial extra-cellular volume (ECV) can be derived, which has been applied to assess myocardial edema and myocardial fibrosis with great clinical value .This review focuses on the techniques , methods and clinical application of cardiac magnetic resonance T 1 mapping technology .

Objective To evaluate exertional overheating and endurance during and after physical exercise on individuals with hypohidrotic ectodermal dysplasia(HED) ,to assess protective effects of skin cooling device ,and to provide theoretical basis for ex-ternal cooling devices .Methods 12 HED patients and 12 age-matched healthy controls were studied during standardized exercise on a treadmill at ambient temperatures of 25 ℃ and 30 ℃ .Body core temperature ,performance ,heart rate ,respiratory rate ,blood pres-sure and serum lactate were investigated during and after exercise .Results HED subjects experienced a significantly greater rise in body temperature after cycling than healthy controls ,and their body temperature remained elevated longer(P<0 .05) .HED subjects had a lower endurance time(F=9 .985 ,P=0 .005) and increasing speed value(F=7 .158 ,P=0 .014) .However ,serum lactate value of the HED subjects found to be higher than the controls (F=5 .204 ,P=0 .033) .Maximum heart rates ,respiratory rate and blood pressure did not differ significantly between HED and the control groups .However ,compared with controls ,body temperature and endurance time of HED patients equipped with skin cooling device had no statistical significance .Conclusion HED subjects showed a significantly greater rise of body temperature during exercise than the control groups ,and their body temperature remained elevat-ed longer than in healthy subjects ,and had a lower performance .External evaporative skin cooling attenuates exertional overheating in HED patients and may facilitate their participation in athletic activities and professional life .

Objective: To study the influence of micrometastasis in lymph node on the prognosis of oral squamous cell carcinoma(OSCC).Methods: In 36 patients micrometastasis in pathologically negative lymph nodes were tested by immunohistochemical cytokeratin(CK) examination and the relationship between CK+ and survival time of the patients was analyzed. Results: The survival time(month) of CK+ and CK- patients was 36.76?6.91 and 47.47?11.35 respectively(P=0.002).Multivariate analysis of Logistic regression model showed that micrometastasis in lymph node (P=0.044)and histologic grade (P=0.040) were regarded as independently prognostic factors while clinical stage (P=0.236)did not. Conclusion: The detection of micrometastasis in the lymph nodes may serve as a supplement to the present staging system of OSCC. The prognosis of patients with micrometastasis is poorer than those without micrometastasis.

Objective: To study the role of simian virus 40 large T antigen(SV40LTag) and telomeras in the proliferation of endothelial cells in hemangioma. Methods: The expression of SV40LTag was examined in 100 cases of hemangioma with immunohistochemistry, telomerase activity and telomeric length were measured by RT-PCR based telomeric repeat amplification protocol(TRAP) assay, telomeric restriction fragment (TRF) analysis and chemiluminescence in situ hybridization in 50 cases of hemangioma and 10 of squamous cell carcinoma. A sample of embryonic kidney endothelia was used as the contrdol. Results: SV40LTag expression was observed in 45 out off the 100 cases of hemangioma. Telomerase expression was found in 27 out off the 50 cases of hemangioma and all the 10 of squamous cell carcinoma. The telomeric length in hemangioma varied from 6.8 to 13.2 kb, 10.5-13.5 kb in squamous cell carcinoma and 12.4 kb in embryonic kidney endothelia. Conclusion: Transformation of endothelia by simian virus 40 may contribute to the occurring of hemangioma, telomerase may play a role in the proliferation of the endothelial cells in hemangioma.