A simple, fast flow injection chemiluminescence (CL) method for the determination of methyl-parathion has been developed. It is based on the reaction of methyl parathion with luminol-H2O2 in the alkaline medium (pH：11.5～12.0), sensitized by water-soluble macromolecule Polyethylene glycol 400. Under the optimal conditions, the CL intensity was linear to the concentration in the range of 5.0×10-8～1.0×10-5　g/mL (r=0.9996), with a detection limit (3σ) of 2.0×10-8　g/mL. Relative standard deviation was less than 4% (n=11) and the recovery was between 82%～93%. This method has been successfully applied to the determination of trace residue of methyl-parathion in grain sample.

Objective To observe the clinical efficacy of acupuncture-moxibustion plusMEI Te-lan’s manipulations in treating lumbar intervertebral disc herniation (LIDH) due to blood stagnation.Method Totally 248 LIDH patients due to blood stagnation were randomized into a treatment group of 123 cases and a control group of 125 cases. The treatment group was intervened by acupuncture-moxibustion plusMEI Te-lan’s manipulations, while the control group was by acupuncture-moxibustion plus tuina at lower back. The short-form McGill Pain Questionnaire (SF-MPQ) and Oswestry disability index (ODI) were observed before and after intervention, and the clinical efficacies were compared between the two groups.Result The total effective rate and recovery-markedly effective rate were respectively 80.5% and 52.0% in the treatment group, versus 72.8% and 20.0% in the control group. There was a significant difference in comparing the recovery-markedly effective rate between the two groups (P<0.05). In the treatment group, the Pain Rating Index (PRI) score, Visual Analogue Scale (VAS) score, Present Pain Intensity (PPI) score, and ODI score were significantly changed after 3-day, 6-day, and 20-day treatment compared to the scores before treatment (P<0.05). After 20-day treatment, the PRI, VAS, PPI, and ODI scores of the treatment group were significantly different from that of the control group (P<0.05).Conclusion Acupuncture-moxibustion plusMEI Te-lan’s manipulations is an effective method in treating LIDH due to blood stagnation.