To put forward spatial resolution detection requirements of 64-slice helical CT based on analyzing the present situation of CT spatial resolution detection. The requirements for spatial resolution in the existing industrial standards were analyzed, and some test data were used to prove the deficiency of the standards. Spatial resolu-tion detection requirements were proposed for 64-slice helical CT, and the factors affecting spatial resolution were ex-plored. The deficiencies in the original standards were eliminated. The proposed requirements may facilitate the spatial resolution detection of 64-slice CT, and thus can enhance image quality for clinical diagnosis and treatment.