1.CaMKⅡ:A molecular switch of synaptic plasticity
Wenbin HE ; Junlong ZHANG ; Longchuan YU ; Naihong CHEN
Chinese Pharmacological Bulletin 2014;(7):897-900,901
Calcium/calmodulin-dependent protein kinase Ⅱ(CaMKⅡ),which is an important protein kinase involved in learning and memory,is found in most tissues,but it is present in especially high concentrations in neurons.The relatively high expression of CaMKⅡin nervous system suggests it plays an im-portant role in the function of nervous system.This paper re-views the research development of the molecular structure of CaMKⅡ and its autophosphorylation,subcellular localization and its role in synaptic plasticity.
2.Effect of long-term sustained release naltrexone on semantic recognition of opioid addicts
Shengxi HE ; Longchuan YU ; Qing CHEN ; Dongmei WANG ; Shu HU ; Shaowei JIA
Chinese Journal of Tissue Engineering Research 2009;13(8):1573-1576
BACKGROUND: Long-term sustained release naltrexone has been reported in clinical application near one year that it can improve emotional state and relieve addiction; therefore, the effect of long-term sustained release naltrexone on memory restoration at neuropsychology level were explored. OBJECTIVE: To observe the effect of long-term sustained release naltrexone on memory ability of opioid addicts. DESIGN, TIME AND SETTING: A contrast observational study was performed at Drug Rehabilitation Centers of Wuhan, Changde, Zhengzhou, and Jiangyang between October and December 2006. Healthy controls were tested in Shenzhen Hospital of Peking University in October 2006. PARTICIPANTS: A total of 88 males with opioid addicts were divided into naltrexone group (n=35), compulsory detoxification group (n=26), and non-treatment group (n=27). Another 22 healthy subjects were considered as the controls. METHODS: At 6-12 months before testing, naltrexone (3.1 g) was subcutaneously implanted into bilateral abdominal wall in the naltrexone group; patients in the compulsory detoxification group underwent completely compulsory detoxification for 6 months, and the examination results, including diamorphine, methadone, and buprenorphine in urine, were negative on the immediately testing day. Event related potential and its wave form were recorded from the opioid addicts in the three groups and from healthy controls who finished semantic recognition between new and old words using portable-type event related potential working system. MAIN OUTCOME MEASURES: Correct rate and response time of semantic memory; latency and amplitude of language related potential-N400. RESULTS: ①There were significant differences in correct rate and response time between three expedmental groups and healthy control group (P < 0.001 ); in addition, correct rate was significantly increased, and response time was significantly shortened in the naltrexone group compared to compulsory detoxification group and non-treatment group (P< 0.05). ② N400 latency in the three experimental groups was significantly longer than healthy control group (P < 0.01), while N400 amplitude in the naltrexone group was increased, which was no significant difference compared to healthy control group (P> 0.05) but was significant difference compared to compulsory detoxification group and non-treatment group (P < 0.01). CONCLUSION: Long-term sustained release naltrexone can effectively improve neural function and enhance semantic memory of the opioid addicts.
3.Genotyping of ABO Blood Group in Partial Population of Yunnan Province by SNaPshot Technology.
Shu Xin YU ; Fa Ming ZENG ; Yan Zhang JIN ; Hong Jing WAN ; Dian ZHAI ; Yu Ming XING ; Bao Wen CHENG
Journal of Forensic Medicine 2017;33(3):277-280
OBJECTIVES:
To detect the genotype of ABO blood group by SNaPshot technology.
METHODS:
DNA were extracted from the peripheral blood samples with known blood groups (obtained by serology) of 107 unrelated individuals in Yunnan. Six SNP loci of the 261th, 297th, 681th, 703th, 802th, and 803th nucleotide positions were detected by SNaPshot Multiplex kit, and relevant genetics parameters were calculated.
RESULTS:
In 107 blood samples, the allele frequencies of types A, B, OA, and OG were 0.355 1, 0.168 2, 0.230 0 and 0.247 6, respectively, while that of types AG and cis AB were not detected. The genotyping results of ABO blood group were consistent with that of serologic testing.
CONCLUSIONS
SNaPshot technology can be adapted for genotyping of ABO blood group.
ABO Blood-Group System/genetics*
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Alleles
;
Asian People/genetics*
;
China
;
DNA
;
Ethnicity
;
Gene Frequency
;
Genotype
;
Humans
;
Polymerase Chain Reaction/methods*
;
Polymorphism, Restriction Fragment Length