OBJECTIVE:To establish a method for content determination of aflatoxins in eupolyphaga. METHODS:HPLC was performed on the column of Phenomenex-C18 with mobile phase of methanol-acetonitrile-water(28∶17∶55,V/V/V)at flow rate of 1.0 ml/min,column temperature was 30 ℃,fluorescence detector(FLD)λex was 365 nm,λem was 450 nm;derivative solution was 0.05% iodine solution,derivatized pump flow rate was 0.3 ml/min,derivatized temperature was 70 ℃ and volume injection was 20μl. RESULTS:Aflatoxin G2 and aflatoxin B2 showed good linear relationship at 1.2-12 pg,and aflatoxin G1 and aflatoxin B1 showed a good linear relationship at 4-40 pg(r≥0.999 3);RSDs of precision,stability and reproducibility tests were≤2.3%;aver-age recovery was 77.4%-94.8%(RSD=3.1%-4.3%,n=6). CONCLUSIONS:The method is simple,accurate and reproducible, and can be used for the determination of aflatoxins in eupolyphaga.