Objective To investigate the expression of CDX2 and its clinical significance in gastric intestinal epithelial tissue,and investigate the relationship between expression of CDX2 and infection of Helicobacter pylori.Methods The expression of CDX2 was studied by immunohistochemical technique in 93 cases of gastric mucosa (including 7 cases of normal gastric mucosa,21 cases of superficial gastritis,22cases of atrophic gastritis,43 cases of atrophic gastritis with intestinal metaplasia).14C urea breath test was used to detect the Helicobacter pylori infection.Results Helicobacter pylori was negative in 7 cases of normal gastric mucosa,the positive rate of Helicobacter pylori infection in atrophic gastritis and atrophic gastritis with intestinal metaplasia was 77.27％ (17/22),65.12％ (28/43),significantly higher than that of superficial gastritis [ 19.05％(4/21 )] (P ＜0.01 ).No expression of CDX2 was observed in normal gastric mucosa and superficial gastritis.The positive rate of CDX2 in atrophic gastritis with intestinal metaplasia was 83.72％(36/43 ),which was higher than that in atrophic gastritis [40.91％(9/22)](P＜ 0.01 ).Conclusions The infection of Helicobacter pylori and the expression of CDX2 are significantly associated with intestinal metaplasia.The abnormal expression of CDX2 might be the origin of intestinal metaplasia.The detection of gastric mucosa of CDX2 expression can be used to identify the intestinal metaplasia of gastric mucosa,predict the progress of gastric precancerous lesions,and provide a basis for the selection of treatment program.

Anticoagulants is the drugs which prevent blood clotting process by affecting coagulation factor.They can be used for prevention of thromboembolic disease and stroke.As we stand on the threshold of a new era of anticoagulants,it is time to take an overview of the different types of anticoagulants and how to use them in the management of some specific conditions,including pregnancy,renal impairment,heparininduced thrombocytopenia and cancer.

Objective To compare the pharmacodynamic actions of Liuwei Dihuang Pills(LDP)prepared by four kinds of techniques on hemolysin contents in mice.Methods Sensitized by SRBC,immuno-inhibited mice models were induced by dexamethasone and cyclophosphane respectively.The hemolysin contents in the mice fed with LDP prepared by four kinds of techniques were observed to compare the pharmacodynamic actions.Results In experiment 1 that immuno-inhibited mice models were induced by cyclophosphane,the half hemolysin concentration(HC50)was low in the model group and LDP groups,especially in the LDP groups of techniqueⅡ,Ⅲ and Ⅳ.In experiment 2 that immuno-inhibited mice models were induced by dexamethasone,the difference of HC50 was insignificant between the blank control group,LDP groups of techniqueⅠand concentrated LDP group,the difference also being not significant in LDP groups of techniqueⅠat different dosages.Conclusion The dose of LDP can be reduced according to the experiment,however,the process technique of LDP needs to be improved.For this prescription is indicated for the treatment of yin-deficiency syndrome,dexamethasone can be used as the proposed drug for the establishment of the immuno-inhibited models.To confirm the reasonability of formula revolution,more parameters should be added to observe the pharmacodynamic actions.

Traumatic brain injury (TBI) is one of the leading causes of death and disability worldwide,particularly in young adults.Conventional imaging modalities such as CT or MRI can show most of the morphological changes of the brain in TBI.PET/CT and PET/MR can acquire both functional and morphological images compared with CT or MRI alone,which might be helpful for the diagnosis,management and prognosis evaluation of TBI.This review summarizes the applications of PET imaging in TBI patients.

Objective To systematically evaluate the risk of BRCA1 mutation in patients with triplenegative breast cancer (TNBC).Methods Articles about the association between the TNBC and BRCA1 mutation were retrieved from database,such as PubMed,CNKI,Wanfang,VIP and CBM databases.The odds ratio (OR) was used to evaluate the risk of BRCA1 mutation in TNBC compared with non-TNBC.Rev Man 5.2 software was applied to perform the Meta-analysis.Results A total of 23 articles were eligible for the Meta-analysis,including 1 104 patients with TNBC and 4 245 patients with non-TNBC.The pooled OR was 7.67 and 95％ CI(6.24,9.42),and the difference was statistically significant (Z =19.38,P ＜ 0.000 01).In the subgroup analysis about the race,the pooled OR for Asian was 6.67 and 95％ CI (4.98,8.95).The pooled OR for Caucasian was 8.83 and 95％ CI (6.61,11.80).There was statistically significant difference in the pooled OR between the Asian and the Caucasian (Z =14.74,P ＜ 0.000 01).Conclusion The patients with TNBC are 7.67 times more likely to have BRCA1 mutation compared with non-TNBC phenotype.Largesample randomized controlled trials are warranted to demonstrate the detection of BRCA1 mutation can be a conven-tional application in clinical for the patients with TNBC.

Objective To investigate clinical effect of file hand stainless steel and nickel -titanium instru-ment combined with sonic endodontic on chronic periapical periodontitis for C -shaped root canals in mandibular sec-ond molars.Methods 140 patients with chronic periapical periodontitis for C -shaped root canals in mandibular sec-ond molar were chosen and randomly divided into two groups,including A group (70 patients)with file hand stainless steel and B group (70patients)with sonic endodontic combined with nickel -titanium instrument.The following -up success rate of root canal therapy,filling quality of root canal,incidence of pain reaction and complication of root canal preparation between the two groups were compared.Results The following -up success rate of root canal therapy in 6 months after treatment of A group and B group were 32.86%(23 /70),67.14%(47 /70).The following -up suc-cess rate of root canal therapy in 12 months after treatment of A group and B group were 64.29%(45 /70),72.86%(51 /70).The following -up success rate of root canal therapy in 6 months after treatment of B group was significantly higher than A group(χ2 =10.27,P <0.05).There was no significant difference in following -up success rate of root canal therapy in 12 months after treatment between the two groups(χ2 =2.14,P >0.05).The good filling rate of root canal of B group was significantly higher than A group(χ2 =9.70,P <0.05).The incidence rates of pain reaction of A group and B group were 48.57%(34 /70),21.43%(15 /70).The incidence rate of pain reaction of B group was significantly lower than A group(χ2 =12.15,P <0.05).The incidence rates of apical foramen deformation,wall perforation and step formation of A group were 6.54%(14 /214),0.93%(2 /214),7.94%(17 /214),those of A group were 7.73%(17 /220),0.91%(2 /220),1.82%(4 /220).The incidence rate of step formation of B group was significantly lower than A group (χ2 =11.14,P <0.05 ).There were no significant differences in the incidence rates of apical foramen deformation and wall perforation between the two groups (χ2 =3.11,1.06,P >0.05 ). Conclusion Nickel -titanium instrument combined with sonic endodontic on chronic periapical periodontitis for C -shaped root canals in mandibular second molars can efficiently improve rational symptoms,enhance filling quality of root canals and reduce pain degree and complication risk,the effect is better than file hand stainless steel for canal preparation.

Objective: To study the effect of eliminating pyrogen with ultrafiltration on the quatity of Chuanshentong Injection. Methods: The sample solution was filtered with the ultrafiltration membranes of molecular weight 6000 and 20000. Then,the results of eliminating pyrogens and the examination results of quality of sample solution before and after ultrafiltration were compared.Results: The results of elminating pyrogens is satisfactory. But there are larger changes in many items of quality markers after ultrafiltration. Conclusion: Therefore, the ultrafiltration should be used with caution in eliminating pyrogens of injection of Chinese medicinals.

Acute Disease ; Humans ; Pneumonia ; prevention & control ; Pulmonary Disease, Chronic Obstructive ; complications ; Respiration, Artificial ; Respiratory Insufficiency ; therapy ; Ventilators, Mechanical ; adverse effects

Objective To explore the effect and mechanism of wortmannin on the human gastric carcinoma cells. Methods SGC7901 cells were treated with 10nmol/L,30nmol/L and 60nmol/L of wortmannin,a specific inhibitor of AKT,for different time periods. Cell viability was estimated by MTT assay. Western blot was used to detect the level of NF-?B neucleoprotein and reverse transcriptasepolymerase chain reaction(RT-PCR) was used to determine transcription of NF-?B mRNA. Results All three different concentrations of wortmannin could inhibit the growth of SGC7901 cells,and the depression effect obviously depended on time and drug dose(P0.05). As the action time of wortmannin was prolonged,neucleoprotein and mRNA expression of NF-?B significantly decreased compared with the control (P

Objective To observe the effect of mirror therapy on acute stroke dysphagia. Methods 60 patients with dysphagia were randomly divided into treatment group (n=30) and control group (n=30). The control group received routine dysphagia rehabilitation and intermittent oral gastric tube feeding, while the treatment group received mirror therapy in addition. They were assessed with Kubota water-drinking test and swallowing videofluoroscopy 4 weeks after treatment, and the incidence of aspiration were recorded. Results The oral transit time, swallowing reaction time, pharyngeal transit time, laryngeal closure duration improved in the treatment group compared with the control group in swallowing videofluoroscopy (P<0.05). The total effective rate was obviously higher in the treatment group than in the control group (P<0.05), with the lower incidence of aspiration pneumonia (P<0.05). Conclusion Mirror therapy may promote the recovery of swallowing function after acute stroke, and reduce the risk of aspiration.