Objective To explore the features of airway inflammation in patients with eosinophilic bronchitis(EB). Methods Sputum induced eosinophilic count was performed in 7 patients with EB(EB group), 12 patients with asthma(asthma group), 16 patients with cough variant asthma(CVA group), and 6 patients with atrophic rhinitis(AR group). The number of eosinophil and mastocyte was detected by bronchial biopsy in patients of EB and CVA group. Results The percentage of eosinophil counts of induced sputum in asthma group, CVA group, EB group and AR group was ( 18.7±5.3)%, ( 10.2± 4.5)% ,(8.7±2.3)% and (2.7±3.2 )% respectively. There were significant differences between EB group and the other three groups.The number of eosinophil in airway smooth muscle was significantly lower in EB group [ ( 1.3±0.1 )/mm2 ]than that in CVA group[ (5.2±1.2)/mm2]. And the mastocyte number in EB group[ (3.5±1.4)/mm2] was significantly lower than that in CVA group [ ( 12.5±2.4)/mm2]. Conclusions Perhaps EB is associated with many diseases. Aggressive treatment maybe prevent asthma. Eosinophil and mastocyte number is significantly low in patients with EB compared those with CVA, which maybe play an important role in absent airway hyper-responsiveness in EB.

Cardiac involvement related to iron overload caused by long-term blood transfusion is a major cause of death for patients with thalassemia major. Patients' survival will be improved greatly if cardiomyopathy resulted from transfusional iron overload could be detected timely and effective iron chelation be initiated promptly. In the present article, various evaluation Methods currently used in clinical practice are reviewed, with particular focus on cardiac MRI for the monitoring of myocardial involvement related to iron overload.

Objective To explore the accuracy and the clinical significance about the diagnosis treatment to allergy rhinitis with bioresonanztherapie.Methods 22 cases who were confirmed as house dust mite,extremely sensitive to duse diagnosed by BICOM were randomly collected and examined by allergic-skin test,which were compared with the control group.Results 22 examples by BICOM bioresonanztherapie,for to the house dust mite and the dust highly sensitive patient,allergy test sets piercing experiment was the strong masculine genders.Conclusion Bioresonanztherapie is really feasible at accuracy and practicability to test allergic rhinitis.

Acetophenones ; pharmacology ; Caspase 3 ; metabolism ; Cell Proliferation ; drug effects ; Cells, Cultured ; Focal Adhesion Protein-Tyrosine Kinases ; metabolism ; Humans ; Muscle, Smooth, Vascular ; drug effects ; metabolism ; pathology ; Pulmonary Artery ; drug effects ; pathology

Antigens, CD1 ; metabolism ; Child, Preschool ; Cholangitis, Sclerosing ; diagnosis ; etiology ; metabolism ; Diagnosis, Differential ; Female ; Histiocytosis, Langerhans-Cell ; complications ; metabolism ; pathology ; Humans ; Immunohistochemistry ; Liver ; metabolism ; pathology ; S100 Proteins ; metabolism

Objective To evaluate the feasibility of cell free fetal DNA(cffDNA)-based noninvasive prenatal diagnosis,we developed a precise technique for fetal sex determining region of Y chromosome(SRY)gene detection using size-fractionated cell-free DNA in maternal plasma.Methods Peripheral blood samples were collected form 117 pregnant women.cffDNA was extracted based on a column absorbent method and isolated by agarose gel electrophoresis.A dulex-polymerase chain reaction(PCR)was used to detected SRY gene and glycerol-dehyde-phosphate dehydrogenase(GAPDH)gene.Results Both SRY and GAPDH gene were detected in 86 cffDNA samples from women bearing male fetuses.And only GAPDH gene was detected in 71 cffDNA samples from women bearing female fetuses.These results had a coincidence whit those of villus or amniotic fluid samples.The specificity and sensitivity reached 100%(117/117)and 100%(66/66),respectively.Conclusion By agarose gel electrophoresis,re-extratedand and dulex PCR,size-fractionated cell-free fetal DNA in maternal plasma can be selective enriched and used to noninvasive prenatal diagnosis of sex-linked disorders and single gene disorders.

Background and purpose: The Testin gene is a candidate tumor suppressor gene located at 7q31.2. Our purpose was to investigate the expression of Testin gene, and its possible relationship with the clinicopathological features of human colorectal carcinoma. Methods: Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry methods were used to investigate the expression of Testin in 60 specimens of colorectal carcinomas and corresponding adjacent normal mucosal tissues. The correlations of Testin to clinicopathologic features of colorectal carcinoma were analyzed. Results: The mRNA level of Testin was down-regulated in colorectal carcinomas. The positive rate of Testin protein was significantly lower than the paracancerous mucosal normal tissues (P<0.01). The expression of Testin protein and mRNA was correlated with infiltration, differentiation, Dukes stage, T stage,lymph node metastasis and distant metastasis (P<0.05), but not with patients' gender, age, site of cancer (P>0.05). The expression of Testin protein was lower in the tissues which were deeper infiltrated, poorer differentiated, advanced in Dukes stage and T stage, or with lymph node or remote metastasis. The expression of Testin mRNA was positively associated with that of Testin protein(r=0.75,P<0.05). Conclusion: The expression level of Testin is significantly down-regulated in colorectal carcinomas, which may be associated with the degree of infiltration, differentiation and metastasis of colorectal carcinoma. It suggests that Testin may be a valuable marker for assessing the prognosis and metastasis of colorectal carcinoma.

Objective:To design and prepare repaglinide and metformin hydrochloride tablets for pilot scale test and industrial pro-duction. Methods:Dissolution was applied to optimize the formula and preparation process. Results: The tablets were prepared ac-cording to the determined formula and process with poloxamer 188, meglumine, PVP K30, croscarmellose sodium, microcrystalline cellulose PH101, sorbitol, magnesium stearate and coating powder as the adjuvants. The dissolution behavior of the tablets in four media was similar to that of the reference. Conclusion:The preparation process of repaglinide and metformin hydrochloride tablets is simple and feasible, and the quality is controllable and stable.

Objectives To evaluate the potential of antisense c myc gene therapy for acute promyelocytic leukemia by investigating the biological effects and molecular mechanisms in induction of differentiation of HL 60 cell line using recombinant antisense c myc adenovirus (Ad AS c myc). Methods Cultured HL 60 cells treated with Ad AS c myc or Ad LacZ with polybrene and protamine sulfate were analyzed by X gal staining, morphology, MTT, flow cytometric analysis, RT PCR, and immunocytochemical techniques in vitro . Results HL 60 cells could be transfected effectively by Ad LacZ+protamine sulfate (79.8%). The level of c myc transcription and the version could be strongly inhibited by Ad AS c myc in the transfected HL 60 cells. Ad AS c myc could strongly inhibit the cell growth in HL 60 cells (51%). Ad AS c myc could reduce the ratio of nuclear/cytoplasm, and increase the activity of peroxidase in HL 60 cells. Ad AS c myc could lead to the blocking of G 0/G 1 phase in HL 60 cells. Ad AS c myc could also increase the expression of c fos in HL 60 cells. Conclusion The expression of Ad AS c myc can inhibit the growth and induce differentiation of HL 60 cells in vitro . The biological effects of Ad AS c myc may be closely associated with the activity of peroxidase and c fos gene in HL 60 cells. Ad AS c myc is of clinical potential in gene thera py for acute promyelocytic leukemia.

Objective To investigate the principles of combined traditional Chinese and western medicine treatment of early postoperation inflammatory small bowel obstruction(EPISBO).Methods 93 cases of EPISBO were randomly divided into two groups including group A,47 cases received combined traditional Chinese and west- ern medicine treatment,and group B 46 received only western medicine treatment.Results 93 patients were all cured through nonsurgical treatment.The combined traditional Chinese and western medicine treatment was better in symtom improvement and reduce of expense.Conclusion The principle treatment of EPISBO is mainly nonsurgical. The traditional Chinese medicine and somatostatin are very important.