Objective To emphasize the necessitya and principle of hearing and speech training after cochlear implantatiom.Methods 2 cases evaluation pre-and post-operation.Results The result of 1 case who carried out effective hearing and speech training post-operation is much better than the result of another one who did not do the training.Conclusion The deaf children can get hearing by cochlear implant.In order to get good rehabilitation result,they should do scientific and effective hearing and speech training.

Objective: To analyze the differential protein expressions in highly-metastatic colorectal carcinoma cell lines LoVo and low-metastatic SW480 cells using proteomic analysis method and detect the biological molecule for early diagnosis of metastasis of colo-rectal carcinoma. Methods: Proteins in highly-metastatic LoVo cells and low-metastatic SW480 cells were isolated by two-dimension gel electrophoresis (Two -DE). Two-DE map was established by gel-silver staining and Coomassie brilliant blue staining. The well differentially expressed protein spots were screened by Image Master imaging. The specific protein spots were identified by peptide mass fingerprints using matrix-assisted laser desorption/time of flight mass spectrometry (MALDI-TOF/MS) and through searching NCBInr database using Mascot software. Results: Twenty one differential protein spots were screened and 5 differential proteins were identified. They included Ezrin protein, transcription elongation factor A, and lamin A/C which were up-regulated in LoVo cells and non-metastatic protein expressed in isoform a (NM23A) and heat shock protein 8 (HSPA8) which were up-regulated in SW480 cells. Conclusion: This study has found that the differential proteins are related with tumor growth, migration, adhesion and apoptosis. Our results provide theoretical basis for finding biological markers for early diagnosis of metastasis of colorectal carcinoma.

Objective To explore the distribution of ABO blood group in Tibetans population and their genetic relationship. Methods Data of ABO blood group of 28 Titetan populations were collected from China and India. The gene frequencies were processed by Phylip3. 68 and MEGA4. 1 genetic analysis software pack, and the Nei's genetic distance was imported and the genetic relationship was analyzed. Results The distribution of ABO blood group among Gansu, Qianghai, Sichuan, Yunnan, Tibet, and India was 0 ＞ A ＞ B ＞ AB. The nation index and genetic distance were 0. 63 ～ 0.98 and 0 ～0. 0072, respectively. Conclusion There is probable historical relationship among the ancestors of these Tibetan populations, but the differentiation incident of Tibetan population living in different area in history was different, so that their distribution of gene frequencies is diversified.

Objective To observe the change of serum TG in elderly diabetic patients after fatty meal.Methods The diabetic patients and non-diabetic patients of fasting normotriglyceridemia ate the fatty test meal after having been fasting for 12h.Serum lipid were measured at 0,2,4 and 6h after the meal.Results The serum TG level increased in the two groups after the meal,the diabetic patients had a higher TG-AUC than the non-diabetic patients (P

Objective To explore the effects of Taiji exercise on the psychology and the functions of the autonomic nervous systems of middle aged and elderly people. Methods Two hundred and seven middle aged and elderly Taiji exercisers and 166 age matched non Taiji exercisers were sampled. The Zung Self rating Anxiety Scale(SAS), Zung Self rating Depression Scale (SDS) and autonomic nervous system equilibrium index (ANSEI)were adopted to assess the subjects. Results Compared with those of the control group, the scores of SAS and SDS in Taiji group were significantly higher, and the equilibrium of autonomic nervous systems in Taiji group was also superior. There was no significant difference between the male and the female with regard to SAS, SDS and the equilibrium of autonomic nervous systems within Taiji group. The longer the exercise, the better the equilibrium of autonomic nervous systems. Conclusion Taiji exercise has positive effects on the psychology and the autonomic nervous system of the middle aged and the elderly.

ObjectiveTo analyze the symmetry of multifocal visual evoked potential (mfVEP) between both eyes in normal subjects. Methods The monocular mfVEP of both eyes in thirty-six normal subjects (72 eyes) was tested with VERIS Science 4.0. The stimulus was the pattern reversal dart array consisted of 60 sectors each included 16 black-white reverse patterns. The visual stimulation was controlled by the binary pseudo-random m-sequences and subtended approximately 25 degrees. Results There existed no statistically significant difference of P1 latencies and amplitudes between correspondent quadrant visual field of both eyes. The data difference of the ipsilateral quadrant visual fields was greater than those of the correspondent quadrant visual field. The comparison among four quadrant visual fields in right eye or left eye each showed that there was statistically significant difference of P1 latencies between the superionasal quadrant visual field and inferiotemporal or inferionasal quadrant visual fields. Conclusions The symmetry of normal mfVEP is more dominant in retina than that in visual cortex.

Objective To investigate relationship between serum uric acid, blood lipids and blood pressure in obesity children.Methods 71 obese volunteers were selected as obesity group and 30 normal volunteers who matched obese volunteers in age and sex as control group from 13 702 children in Xiangtan city by sampling survey. Their body mass index(BMI), systolic and diastolic blood pressure(SBP,DBP),serum uric acid(UA), high-sensitive C-reactive protein(hs-CRP) and blood lipids were checked respectively. Then ,the difference between the two groups was compared and the relationship of all indexes was analyzed, then the difference between the two groups was compared and the relationship of all indexes was analyzed.Results The values of serum UA,hs-CRP, triglyceride(TG),low density lipoprotein cholesterol(LDL-C) and apolipoprotein B(apoB) in obesity group were significantly higher than those in control group (P

Objective To explore the safety and feasibility of laparoscopy in diagnosis and treatment of neonates and infants diseases.Methods Laparoscopic operations were performed in 297 neonates and infants aging from 2 hours to 6 months,from January 2001 to May 2007.The diseases of 297 cases included 80 cases of congenital hypertrophic pyloric stenosis,67 cases of obstructive jaundice,35 cases of indirect inguinal hernia,31 cases of biliary atresia,21 cases of middle or high anal atresia,21 cases of biliary hyperviscosity,7 cases of congenital diaphragmatic hernia,7 cases of jejunal atresia,6 cases of choledochal cyst,5 cases of Hirschsprung's disease,5 cases of duodenal web,3 cases of uronephrosis,2 cases of esophageal hernia,2 cases of esophageal atresia,2 cases of intestinal malrotation,1 case of intestinal obstruction,1 case of intussuception,,and 1 case of congenital muscular troticollis.Results The laparoscopic operations were completed in 294 cases,of which 67 received diagnostic cholangiography,80 received pyloromyctomy,35 received hernia sac high ligation,21 received biliary irrigation,5 received operations for membranous stenosis of duodenum,2 received Ladd operations for malrotation of intestine,1 received reduction of intussusception,1 received enterolysis,and 1 received incision of lower end of sternocleidomastoid muscle;and some complicated procedures including 31 cases of hepatic portal duct-jejunostomy,21 anoplasty,7 repair of diaphragmatic hernia,7 enterectomy and entero-anastomosis of intestinal atresia,6 hepatic duct-jejunostomy,5 radical surgery for Hirschsprung's disease(megadolicho-colon),3 pyeloplasty,2 operations for esophageal atresia,and 2 fundoplication for hiatus hernia.No massive hemorrhage,intra-or post-operative blood infusion,accidental damage or peri-operative death was reported.Three cases of biliary atresia were converted to open surgery due to oozing of blood in hepatic portal.All case were followed for 2 months-6 years,and no long-term or short-term complication was found.Conclusions Laparoscopic technique is safe and reliable in the diagnosis and treatment of diseases in neonates and infants,which is characterized by minimal invasion,quick recovery and satisfactory cosmetic effects.

Objective In order to protect the natural resources of Swertia bimaculata which has been destroyed seriously,the method of artificial propagation by way of tissue culture have been systematically studied.Methods The stems,leaves,and stems with buds which were from the seedlings germinated from the seeds on the initial medium were taken as explants.These explants were cultured on MS culture media by adding different portions of hormones at various cultural conditions.Results The stems were the best material in speeding propagation among the three explants.The proper initial medium for the stems was MS+ BA 0.5 mg/L+saccharose 3.0%,the optimum medium for proliferation was MS+BA 0.5 mg/L+IBA 0.1 mg/L+saccharose 3.0%,and the best medium for rooting was 1/2MS+NAA 0.5 mg/L+saccharose 1.5%.Conclusion Tissue culture of S.bimaculata could make its propagation rapid,its resources preserved,and its utilization last.