Objective To emphasize the necessitya and principle of hearing and speech training after cochlear implantatiom.Methods 2 cases evaluation pre-and post-operation.Results The result of 1 case who carried out effective hearing and speech training post-operation is much better than the result of another one who did not do the training.Conclusion The deaf children can get hearing by cochlear implant.In order to get good rehabilitation result,they should do scientific and effective hearing and speech training.

Objective: To analyze the differential protein expressions in highly-metastatic colorectal carcinoma cell lines LoVo and low-metastatic SW480 cells using proteomic analysis method and detect the biological molecule for early diagnosis of metastasis of colo-rectal carcinoma. Methods: Proteins in highly-metastatic LoVo cells and low-metastatic SW480 cells were isolated by two-dimension gel electrophoresis (Two -DE). Two-DE map was established by gel-silver staining and Coomassie brilliant blue staining. The well differentially expressed protein spots were screened by Image Master imaging. The specific protein spots were identified by peptide mass fingerprints using matrix-assisted laser desorption/time of flight mass spectrometry (MALDI-TOF/MS) and through searching NCBInr database using Mascot software. Results: Twenty one differential protein spots were screened and 5 differential proteins were identified. They included Ezrin protein, transcription elongation factor A, and lamin A/C which were up-regulated in LoVo cells and non-metastatic protein expressed in isoform a (NM23A) and heat shock protein 8 (HSPA8) which were up-regulated in SW480 cells. Conclusion: This study has found that the differential proteins are related with tumor growth, migration, adhesion and apoptosis. Our results provide theoretical basis for finding biological markers for early diagnosis of metastasis of colorectal carcinoma.

Objective To explore the distribution of ABO blood group in Tibetans population and their genetic relationship. Methods Data of ABO blood group of 28 Titetan populations were collected from China and India. The gene frequencies were processed by Phylip3. 68 and MEGA4. 1 genetic analysis software pack, and the Nei's genetic distance was imported and the genetic relationship was analyzed. Results The distribution of ABO blood group among Gansu, Qianghai, Sichuan, Yunnan, Tibet, and India was 0 ＞ A ＞ B ＞ AB. The nation index and genetic distance were 0. 63 ～ 0.98 and 0 ～0. 0072, respectively. Conclusion There is probable historical relationship among the ancestors of these Tibetan populations, but the differentiation incident of Tibetan population living in different area in history was different, so that their distribution of gene frequencies is diversified.

Objective To investigate relationship between serum uric acid, blood lipids and blood pressure in obesity children.Methods 71 obese volunteers were selected as obesity group and 30 normal volunteers who matched obese volunteers in age and sex as control group from 13 702 children in Xiangtan city by sampling survey. Their body mass index(BMI), systolic and diastolic blood pressure(SBP,DBP),serum uric acid(UA), high-sensitive C-reactive protein(hs-CRP) and blood lipids were checked respectively. Then ,the difference between the two groups was compared and the relationship of all indexes was analyzed, then the difference between the two groups was compared and the relationship of all indexes was analyzed.Results The values of serum UA,hs-CRP, triglyceride(TG),low density lipoprotein cholesterol(LDL-C) and apolipoprotein B(apoB) in obesity group were significantly higher than those in control group (P

With the development of radiotherapy techniques,the efficacy of radiotherapy for liver tumors has been improved,and radiothera-py can be considered as an alternative treatment for patients who are not suitable for surgery.The progress in liver tumor radiotherapy repor-ted in the 55th annual meeting of the American Society for Therapeutic Radiology and Oncology (ASTRO)in 2013,particularly the efficacy and safety of stereotactic body radiation therapy for liver tumors,is reviewed.In the future,radiotherapy may be increasingly used as an ef-fective method in the treatment of liver tumors.The main studies reported at the meeting are sorted as follows in this article.

Objective To investigate the clinical efficacy of laparoscopic splenectomy combined with pericardial devascularization.Methods The retrospective cross-sectional descriptive study was adopted.The clinical data of 64 patients who underwent laparoscopic splenectomy combined with pericardial devascularization at the Second Affiliated Hospital of Kunming Medical University from April 2012 to June 2015 were collected.Observed indexes included (1) treatment outcomes,including surgical procedures,operation time,volume of intraoperative blood loss,time of postoperative enteral recovery,time of postoperative drainage tube removal,duration of postoperative hospital stay,occurrence of complications,(2) follow-up situation.The follow-up using reexaminations of blood routine,liver function,coagulation function,gastroscopy and color Doppler ultrasonography of portal vein was performed regularly at postoperative month 1,2,3,6,12,24 until reemergence of gastrointestinal hemorrhage.The final deadline was death of patients and performance of liver transplantation.Measurement data with normal distribution were presented as-x ± s.Results (1) Treatment outcomes:of 64 patients,62 underwent total laparoscopic splenectomy combined with pericardial devascularization successfully.One patient was transffered to hand-assisted laparoscopic splenectomy combined with pericardial devascularization due to uncontrollable hemorrhage.One patient received laparoscopic cholecystectomy firstly with volume of blood loss of about 1 500 mL and terminated surgery after infusion of suspension red blood cells of 6 U and plasma of 900 mL,and underwent laparoscopic splenectomy combined with pericardial devascularization again next week.No postoperative hemorrhage,pancreatic leakage or death occurred during the perioperative period.The operation time,volume of intraoperative blood loss,time of postoperative enteral recovery,time of postoperative drainage tube removal and duration of postoperative hospital stay were (146 ± 33) minutes,(214 ± 31) mL,(24 ± 4) hours,7 days and (14 ± 6) days,respectively.Nine patients had postoperative pleural effusion and recovered after thoracic drainage and thoracentesis.(2) Follow-up situation:All the 64 patients were followed up for an average time of 19.7 months (range,3.0-23.0 months).Reexamination of gastroscopy showed improvement of varicosed veins of lower esophagus and fundus of stomach.During the postoperative 3 months,4 patients had portal vein thrombosis with level of PLT ＞ 700 × 109/L.For patients with D-Dimer ＞ 5,low molecular weight heparin of 0.4 U was injected subcutaneously until D-Dimer ＜ 2.Three patients were loss to follow-up at postoperative month 6 without upper gastrointestinal hemorrhage,hepatic encephalopathy or liver failure.Conclusion Laparoscopic splenectomy combined with pericardial devascularization is safe and effective for portal hypertension,and rigorous perioperative management offers guarantee for surgical safety.

Objective To investigate the effect of combined application of mannitol and monosialoganglioside on neurological function and its efficacy in brain edema after intracerebral hemorrhage. Methods 92 cases of cerebral edema in patients with cerebral hemorrhage in our hospital, were randomly divided into groups, each group of 46 cases, the control group on the basis of the treatment of mannitol (125mL per times, once per 8h) treatment, the study group on the basic of control group received monosialogangliosides (20mg per times, once daily), 10d for a course of treatment, determination of serum indexes, neurological functions were recorded. Results The effective rate of control group was 71.74%, which was lower than 91.30% of the study group, there was significant difference (P < 0.05); compared with control group after treatment , in study group the intracranial pressure, intracranial hematoma, edema decreased, the urine volume increased, National Institutes of Health Stroke Scale (NIHSS) and quality of life score decreased, interleukin (IL-1β), high sensitive C reaction protein (Hs-CRP), IL-6 and IL-8 levels decreased, Na+-K+-ATP enzyme increased, the ratio of cerebrospinal fluid albumin and serum albumin, matrix metalloproteinase (MMP-2) and MMP-9 decreased, there were significant differences (P < 0.05). Conclusion The effect of the combined application of mannitol and monosialoganglioside on cerebral edema after cerebral hemorrhage is exact and could improve neurologic function.

Objective To discuss the effect of nicotine on the growth of rabbit tendon cells.Method 1% concetration Nicotine(0 5ml/kg) was injected into rabbit's auditive vein,then femoral vein blood was extracted at 3 minutes later. Separating blood serum and add it into F-12 medium and 10% fetal bull serum,penicillin,streptomycin,vitC which was then used to culture rabbit tendon cells, observating the time of cell number growth duplication of test group and drawn up its growth curve. The control group was treated as the test group except the rabbit was not injected by nicotine.Results The time of cell number growth duplication of test group was 9?2 1 days and the control group was 7?1 1 days,there was difference significatly between the two groups.Conclusion Nicotine can restrain the growth of rabbit tendon cells and enhance tendon's adhesiveness in post-operation.

50%),including 22 complicated with hypertension cases were retrospectively analyzed and statistics their fluid infusion volume,serum albumin levels serum sodium,blood sugar and general infections conditions were summarized,and compared with non-hypertension cases.Results The difference of fluid infusion volume between them was no significance in statistics.The difference of serum Na +,blood sugar,serum albumin levels between them was significant.All of 22 cases of hypertension had toxemia symptom,12 of 22 cases in control group occured toxemia symptom,morbidity peak of hypertension was accorded with infection morbidity peak.Conclusions General infection after burns could play an important role during the happening of hypertension after burns. We should pay more attention to the therapy of infection in treatment period.