Objective To study the effect of amikacin on aquaporin 4 (AQP4) expression in mice inner ear and reveal the possible mechanism of amikacin ototoxicity .Methods A total of 60 CBA/CaJ mice were randomly di-vided into experimental group and control group with 30 mice in each group .Experimental group mice were subcuta-neously injected with 450 mg/kg amikacin once a day for 2 weeks ,meanwhile control group mice were injected with normal saline .The expression of AQP4 were detected by immunohistochemistry .The changes of AQP4 protein and mRNA abundance were detected separately by Westen Blots and RT -PCR .Results AQP4 in mice inner ear mainly located in supported cells in Corti’s organ .AQP4 protein abundance in experimental group and control group were 0 .672 ± 0 .074 and 0 .479 ± 0 .108 ,mRNA abundance were 0 .701 ± 0 .107 and 0 .460 ± 0 .080 ,respectively .The a-bundance of AQP4 protein and mRNA in inner ear in amikacin -treated mice were significantly lower than those of in control group .Conclusion Amikacin may down -regulate AQP4 expression in mice inner ear .

Objective To explore the effects of husband and wife joint education model on marriage adjustment and birth and quality of life in female patients with infertility. Methods Totally 360 sterile females who received In Vitro Fertilization-Embryo Transfer (IVF-ET) in the Fertility Dpartment of a Class Ⅲ Grade A hospital in Wenzhou were selected using convenient sampling from June to August 2018. The study subjects were randomly divided into intervention (n=180) and control (n=180) groups. The conventional nursing method was used in the control group, and the husband and wife joint education model was applied in the intervention group after the conventional nursing. On the basis of transplantation, the dyadic adjustment scale and birth and quality of life scale were used before the patients were discharged to compare the result of marriage adjustment and birth and quality of life in the two groups. Results The martial adjustment score and birth and quality of life score in the intervention group scored (114.56±20.64) and (68.05±15.18) respectively, both higher than those of the control group, and there was statistically significant difference between the two groups (P＜0.05). Conclusions Husband and wife joint education model can not only promote the intimate relationship between patients and their spouse, but also improve their quality of life, which is worth applying in the clinical care of patients with infertility.