To evaluate the clinical effects of metronidazole-doxycycline gel for treatment of periodontitis, 18 adult patients with periodonti-tis, who had at least 4 mm of probing depth (PD) on the symmetric quadrant, were selected to participate in a randomised study with split-mouth design. Patients of the experimental group were given with metronidazole-doxycycline gel locally once a day for 7 days,while those of the control group were given with metronidazoce stilus. PD, PLI(plaque index), GI(gingival index), BI(bleeding index) were examined on days 0 and 7 of treatment. The results showed that all clinical parametres of the experimental group were better than those of the control group . Therefore, metronidazole-doxycycline gel is effective in the treatment of periodontitis.

Aim To explore the effect of genetic poly-morphisms of POR on the stable warfarin maintenance doses in Han Chinese patients receiving mechanical heart valve replacement. Methods The association between POR gene polymorphisms and warfarin doses of 185 Han Chinese patients were investigated through ANOVA or t test. SNPs of POR and VKORC1 were de-tected by Sequenom? DNA MassArray genotyping method. CYP2C9*3 was genotyped by polymerase chain reaction-restriction fragment length polymorphism method ( PCR-RFLP ) . Patients ’ clinical characteris-tics, INR value and daily dose were obtained from their medical records. Statistical analysis was performed by SPSS 21. 0 software. Results No mutant carriers of POR rs17148944 , POR rs56256515 and rs72553971 were found in this study. The genotype frequencies of other SNPs were in accordance with Hardy-Weinberg e-quilibrium. In the group of patients with CYP2C9*1*1 , the mutant type carriers ( T carriers ) of POR rs17685 had a significantly higher dose than CC carri-ers(3. 50 ± 1. 07) mg·d-1 vs (3. 14 ± 0. 94) mg· d-1,P =0. 03. Also, in the group of patients with CYP2 C9*1*1 and VKORC1 rs9934438 G allele carri-ers, the mutant type carriers ( T carriers ) of POR rs17685 had a significantly higher dose than CC carri-ers(4. 76 ± 0. 90) mg·d-1 vs (4. 08 ± 1. 03) mg· d-1 ,P=0. 04. No significant difference was found in different genotypes of POR rs2868177 . Conclusion These results illustrate that POR rs17685 T carrier is closely associated with a higher warfarin maintenance dose, suggesting that this SNP is useful for clinical guidance of warfarin.

Objective To investigate the inhibitory effect of the cytochrome P450 3A4(CYP3A4) gene expression and function in CHL-3A4 cells lines by vector-ecpressed small hairpin interfering RNA(shRNA).Methods The shRNA expression vectors targeting CYP 3A4 gene(CYP3A4Ⅰ,CYP3A4Ⅱ,CYP3A4Ⅲ) were designed and constructed.The inhibitory effect of shRNA was detected by Western blot and RT-PCR analysis.The inhibitory effect of cytotoxic of cyclophosphamide using shRNA was measured by MTT assay.Results CYP3A4Ⅲ shRNA expressing vector significantly reduced the CYP3A4 mRNA(70%) and protein expression levels(75%) of the CYP3A4 gene in CHL3A4 cells suppression of CYP3A4 gene expression by CYP3A4Ⅲ largely reversed cyclophosphamide cytotoxicity(75%) in CHL-3A4 cells.Conclusion Vector-based RNAi could suppress CYP3A4 gene expression and function,and the use of RNAi to inhibit CYP3A4 gene expression in mammalian cells was a promising new tool for the study of cytochrome P450 gene function.

Objective To explore the effects of husband and wife joint education model on marriage adjustment and birth and quality of life in female patients with infertility. Methods Totally 360 sterile females who received In Vitro Fertilization-Embryo Transfer (IVF-ET) in the Fertility Dpartment of a Class Ⅲ Grade A hospital in Wenzhou were selected using convenient sampling from June to August 2018. The study subjects were randomly divided into intervention (n=180) and control (n=180) groups. The conventional nursing method was used in the control group, and the husband and wife joint education model was applied in the intervention group after the conventional nursing. On the basis of transplantation, the dyadic adjustment scale and birth and quality of life scale were used before the patients were discharged to compare the result of marriage adjustment and birth and quality of life in the two groups. Results The martial adjustment score and birth and quality of life score in the intervention group scored (114.56±20.64) and (68.05±15.18) respectively, both higher than those of the control group, and there was statistically significant difference between the two groups (P＜0.05). Conclusions Husband and wife joint education model can not only promote the intimate relationship between patients and their spouse, but also improve their quality of life, which is worth applying in the clinical care of patients with infertility.