Objective To explore the effects of home-based 6-minute walk test on self-management in patients after percutaneous coronary intervention (PCI). Methods Eighty-four patients who admitted for PCI in the past five years in Cardiovascular Medicine of Zhujiang Hospital of Southern Medical University were recruited from the three major communities and were divided into intervention group and control group by random digits table method with 42 cases each. The intervention group had the home-based 6-minute walk test and monthly telephone follow-up while the control group was followed up by telephone and keep daily activities. Six months after the intervention,the changes of chronic disease self-management in-ventory were compared. Results There were significant differences the overall health self-assessment, fa-tigue,chest pain, time of aerobic exercise, the increase of self-efficacy between intervention group and control group[ (0.62±1.28) points vs. (0.20±1.71) points, (-0.84±3.27) points vs. (0.36±2.71) points, (0.04 ±3.56) points vs. (0.34 ±3.56) points, (30.47 ±123.29) min/week vs. (6.62 ±84.82) min/week, (0.42±3.01) points vs. (-0.29±2.94) points ], and there were significant differences, F=9.15, 7.42, 5.24, 3.91, 12.18, P<0.01. But there were no significant differences for other 9 aspects between two groups (P>0.05). Conclusions Home-based 6-minute walk test can be a simple self-assessment tool used at home for patients after PCI.

Objective:To investigate the feasibility of dynamic turbidity method for the detection of bacterial endotoxin content in influenza split vaccine. Methods: According to the bacterial endotoxin detection method described in general rule 1143 in Chinese Pharmacopoeia (2015 edition), the reliability test for standard curves of influenza split vaccine, the interference initial screening test, the interference verification test and the endotoxin content were performed or determined, and the results were compared with those by the gel method for the same batches of vaccine. Results:The results of reliability test for standard curves were accordance with the reg-ulations. In the interference initial screening test, vaccine was diluted by 160 times, 320 times and 640 times, and the recovery was between 50% and 200%, which showed no interference. The results of the interference verification test further proved that vaccine with 640 times dilution had no interference effect on test. The bacterial endotoxin contents of 10 batches of influenza split vaccine deter-mined by the turbidity method were less than the limit value of 20 EU·ml-1 , and the results were the same as those determined by the gel method. Conclusion:It is feasible to detect the content of bacterial endotoxin in influenza split vaccine by the dynamic turbidity method, which is worthy of promoted application.

Objective To study the effect and mechanisms of berberine (BBR) on the proliferation of papillary thyroid cancer K1 cells induced by high glucose.Methods K1 cells were cultured under 5.5 mmol/L or 25 mmol/L glucose condition with or without different concentration of BBR (0,10,40 and 80 μmol/L) for 24 hours.The proliferations of K1 cells in each condition were detected by MTT.Western blot was used to measure the expression of nuclear factor erythroid 2-related factor 2 (Nrt2),phosphoinositide 3-kinase (PI3K),protein kinase B (Akt) and phosphorylated-Akt (p-Akt).The distribution pattern of Nrf2 in K1 cells was determined using immunofluorescent staining.Results Compared with 5.5 mmol/L condition,the proliferation rate [(126.64 ± 5.41) ％ vs (87.31 ± 3.67) ％],expression levels of PI3K (0.425 ±0.019 vs 0.272 ±0.039),p-Akt/Akt (0.446 ±0.021 vs 0.168 ±0.035) and Nrf2 (0.597 ± 0.014 vs 0.308 ± 0.026),and Nrf2 distribution (93.0％ vs 23.1％) in nuclear of K 1 cells under 25 mmol/L condition were significantly elevated,respectively (all P ＜0.01).Addition of BBR in 25 mmol/L condition dose dependently (10,40,80 μmol/L) lowered the proliferation rate of K1 cells [(111.76 ± 4.10)％,(70.03 ±2.18)％,(32.41 ±3.76)％ vs (126.64 ±5.41)％,all P＜0.05],and suppressed the expression of PI3K,p-Akt/Akt,Nrf2,and Nrf2 nuclear distribution (P ＜ 0.05).Conclusions BBR dose dependently inhibited the proliferation of high glucose-induced K1 cells.This effect was associated with the suppression on of PI3K/Akt signaling activation,Nrf2 expression and its nuclear translocation.