BACKGROUND:Safety of self-made silicon carbide ceramic(SiC)was evaluated before clinical application.OBJECTIVE:To study the biocornpatibility of low-Ni non-Be SiC alloy,detect its teratogenic effect to the mice with micronuclei test.DESIGN,TIME AND SETTING:Randomized controlled animal study was performed at Central Laboratory of China Medical Universitv from June tO December 2007.MATERIALS:86 mice were randomly divided into 8 groups by sex and body mass,including SiC(0.02,0.002,0.000 2 mL/kg)groups,Ni-Cr-Ni extract(0.02,0.002,0.000 2 mL/kg)groups,saline group,and 75 mg/kgcyclophosphamide group.with 10 mice in each group.METHODS:Mice in each group were administrated once and sacrificed after 24 hours.Bone marrow extracted from bilateral femur was used to make cell suspension.Slide specimens were fixed,dried,and dipped into Giemsa,The district which had complete cell,well-distributed,and well-coloration,was selected and counted under light mlcroscope,MAIN OUTCOME MEASURES:1000 micronucleated cells of polychromatic erythro-cytes(MNPCE)were counted,and ratio between polychromatic erythro-cytes(PCE)and normochromatic erythrocytes(NRBC),i.e.,P/N,wascalculated.RESULTS:Micronuclear rates in the cyclophosphamide group were higher than other groups(P＜0.01):however,there wete no significant differences in micronuclear rates among SiC ceramic group,Ni-Cr-Ni extract group,and saline group(P＞0.05);while,there were also no significant differences between SiC ceramic group and Ni-Cr-Ni extract grouP.The P/N value was≥1.CONCLUSION:The self-made SiC has no teratogenic effect to mice and shows great biocompatibility

Seventy patients, who received type Ⅱ restoration of mandibular first molars in Department of Stomatology, Third People's Hospital of Shenyang, were selected and randomly divided into 2 groups, which were treated with silver amalgam filling and cobalt-chromium inlay, separately. The patients were followed up for 2 years to evaluate clinical outcomes such as marginal color, marginal adaptation, contour, secondary caries, and breakage of dental prosthetic restoration. The findings showed that cobalt-chromium inlay had better effect in marginal color, marginal adaptation and contour in class Ⅱcavity restoration than silver amalgam filling, and no material reaction or host reaction was found by two methods. This indicates that both kinds of materials have good biocompatibility, and cobalt-chromium inlay in the restoration of dental defect shows a more promising prospect.

WIF-1 is an important antagonist in the Wnt/?-catenin pathway.As a tumor suppressor, WIF-1 is down-regulated in majority cancers but when it is correlated with WIF-1 promoter hypermethylation and treated with demethylation drug, its expression can be restored.Functional loss of WIF-1 due to promoter hypermethylation plays an important role in a tumor's pathogenesis, possibly mainly through aberrant canonical Wnt/?-catenin signal activation.The correlation of the expression of WIF-1 with clinicopathological features in different cancers is not identical.Detecting WIF-1 and promoter hypermethylation may offer help for early diagnosis, therapy and predicting prognoses.

Objective To investigate neurogenesis in the dentate gyrus of adult rat following conditioned fear stimulus.Methods 24 adult male rats were randomly divided into conditioned fear stimulus group and control group,and all rats were intraperitoneally injected bromodeoxyuridine(BrdU)3 days before conditioned fear stimuli.Animals in conditioned fear stimulus group were given monotone matching foot shock and made conditioned fear stimulus model,animals in control group only give monotone.Freezing time was detected before stimuli and 7th,14th days after stimuli,then rats were sacrificed 7th or 14th day after fear conditioned stimulus,and immunohistochemistry for Brdu was detected.Results Behavioral assessment showed that the percents of freezing time in conditioned fear stimulus group 7th,14th days after conditioned fear stimulus were significantly high(88.68%,91.33%)than those in control group(11.18%,14.23%,P<0.01)and before stimulus(24.11%,16.11%,P<0.01).Compared to control group(1567±28,580±5),Brdu positive cells in dentate gyrus of rat in conditioned fear stimulus group(1364±21,296±22)were decreased,and it reached to a statistical significance on 14th day (P<0.05).Conclusion The decreased neurogenesis in the dentate gyrus of adult rat following conditioned fear stimuli may have relation to fear and other emotion memory.

BACKGROUND: There have been no reports in CNKI database addressing the cellular apoptosis caused by stainless steel materials used in the dental magnetic attachment. OBJECTIVE: To observe the effects of 3 stainless steel materials used in the dental magnetic attachment, cobalt-chromium alloy, titanium alloy, and austenitic stainless steel on L-929 cell apoptosis. DESIGN, TIME AND SETTING: A comparative observation was performed at the Central Laboratory of China Medical University between June and December 2007. MATERIALS: Mouse L-929 fibroblasts in logarithmic growth phase were provided by Institute of Tumor, First Affiliated Hospital of China Medical University, China. Cobalt-chromium (Co-Cr) alloy was purchased from Heraeus-Kulzer Corporation, China. Titanium alloy was obtained from Morita Company, Japan. Austenitio stainless steel was provided by Institute of Mental Research Chinese Academy of Sciences, China. METHODS: The leaching liquor of Co-Cr alloy, titanium alloy, and austenitic stainless steel was made by culturing 3 kinds of round-slice mental materials in 24-well plate with RPMI-1640 medium at 0.1 mL/cm2 (leaching liquor volume: specimen surface area). L-929 fibroblasts were divided into 5 groups: Co-Cr alloy, titanium alloy, austenitic stainless steel, negative control, and positive control. The Co-Cr alloy, titanium alloy, and austenitic stainless steel groups were cultured with corresponding leaching liquor (250, 500, and 1 000 g/L). Cells from the negative control and positive control groups were cultured with simple RPMI 1640 medium and 100 mg/L mitomycin-treated L-929 cells. MAIN OUTCOME MEASURES: Following 24-hour culture, the effects of 3 kinds of leaching liquor (at different concentrations) on L-929 fibroblasts were examined through the use of flow cytometer. RESULTS: There was significant difference in cellular apoptosis between Co-Cr alloy, titanium alloy, and austenitic stainless steel groups at the same concentration or between different concentrations for the same dental material (P < 0.01), but no significant difference existed between titanium-alloy (250 g/L) and negative control groups (P > 0.05). CONCLUSION: Co-Cr alloy, titanium alloy, and austenitic stainless steel have apparent influence on cellular apoptosis. The apoptosis rate is the greatest in the Co-Cr alloy group, followed by austenitic stainless steel group, and lastly titanium alloy group.

OBJECTIVE To determine the sensitivity of Vibrio vulnificus to 23 kinds of antibacterial drugs,and offer the evidence for clinical V.vulnificus infections treatment.METHODS Isolated 11 strains of V.vulnificus from the patients blood and blisters of infected parts,using ATB automatic identification and susceptibility testing to measure the vitro drug sensitivity of the bacteria.RESULTS The susceptibility of the bacteria to the 22 kinds of commonly used antibiotics showed 100% sensitivity,the rate of ciprofloxacin sensitivity was 72%.CONCLUSIONS It is sensitive to commonly used semi-synthetic penicillin,cephalomycin,carbapenem,aminoglycosides,the third generation cephalosporins,fluoroquinolones and so on,all these above drugs may be chosen to treat the V.vulnificus infection.

Objective To evaluate the effect of treatment with batroxobin on above moderate degree of sudden hearing loss (SHL) ,in order to achieve better clinical therapy .Methods The clinical records of 56 patients (57 ears) with above moderate de‐gree of SHL hospitalized from October 2010 to October 2011 were retrospectively studied .57 ears contained 22 ears of flat type and 35 ears of total type ,33 ears were treated with conventional drugs(conventional group) ,while 24 ears were treated with batroxobin and conventional drugs(combination group) .Results The differences of the total effective rate were statistically significant be‐tween conventional group and conventiona group(P=0 .012 8) and between flat type group and total type group(P=0 .012 6) . Conclusion The combination therapy with batroxobin and conventional drugs can significantly increase the total effective rate of the patients with above moderate degree of SHL ,which is superior to conventional drugs .The prognosis of patients with total type is poor than flat type .

Objective:To evaluate the role of HPV16E6,cyclin D1,and human telomerase transcriptase(hTERT) in the development and progression of nasopharyngeal carcinoma(NPC) and to discuss the clinical significance.Methods: Immunohistochemistry was used to detect the expression of HPV16E6,cyclin D1,and hTERT in paraffin-embedded nasopharyngeal carcinoma tissues and nasopharyngeal chronic inflammation tissues.The relationship between their expression with the clinicopathological features of NPC was analyzed;the influence of their expression on prognoses of patients was also analyzed.Results: The positive rates of HPV16E6,cyclin D1,and hTERT in NPC tissues were 62.5%(35/56),50.0%(28/56),and 67.9%(38/56),respectively,which were significantly higher than those in the inflammation tissues(P0.05).HPV16E6 expression was positively correlated with cyclin D1(r=0.480,P

OBJECTIVE: To investigate the expression of Forkhead Box M1 transcription factor (FoxM1) mRNA and protein in laryngeal squamous cell carcinoma (LSCC) and its clinical significance. METHOD: Immunohistochemistry was used to examine the expression of FoxM1 in 89 LSCC tissues, 89 adjacent normal tissues and 20 laryngeal papilloma (LP) tissues. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to examine the expression of FoxM1 in 20 LSCC tissues, 20 LP tissues and 20 adjacent normal tissues. The relationship between FoxM1 expression and the clinicopathological parameters of LSCC were analyzed. RESULT: FoxM1 mRNA and protein expression were gradually decreased from LSCC to LP and normal tissues. The difference was significant (P<0.05). There was a significantly correlation between FoxM1 and histologic differentiation, T stage, lymph node metastasis and clinical stage. Tumors with poorer differentiation, in higher T stage, with lymph node metastasis or in higher clinical stage presented higher FoxM1 expression than tumors with better differentiation, in lower T stage, without lymph node metastasis or in lower clinical stage (P<0.05). The positive expression of FoxM1 was not related with the age, gender or primary site of LSCC patients (P>0.05). CONCLUSION The up-regulation of FoxM1 may play an important role in the invasion and metastasis of LSCC.
Adult ; Aged ; Aged, 80 and over ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Female ; Forkhead Box Protein M1 ; Forkhead Transcription Factors ; metabolism ; Humans ; Laryngeal Neoplasms ; metabolism ; pathology ; Male ; Middle Aged ; Prognosis

Objective To change methods of experiments for increasing Light Cycler's ability to seek out samples of function.Methods To increase the quantity of the reaction of substance,proceed to amplification in advance,and increase the seeking temperature etc.Results Above methods affects inapparent to the accuracy of the examination result,the method of increasing the seeking temperature is particularly simple and practical.Conclusion The inside clinical laboratory science process can adopt the above methods to increase the LightCycler's seeking-out function according to actual circumstance.