PSP94 TNF?D11a naked plasmid intramuscular injection on intact mice.PSP94 TNF?D11a cDNA cloned in pcDNA3,an eukarocytic experssion plasmid,was intramuscularly injected into the quadriceps muscle of 39 mice (50?g each). Serum was collected .The expression of fusion protein was detected by ELISA.Uninjected mice were used as control.RNA of PSP94 TNF?D11a from tissue of skeletal muscle was determined using reverse transcription polymerase chain reaction analysis.The result showed that the levels of expressed protein were remarkably elevated in the serum nine days after the injection of naked plasmid DNA ,peaking on the fourteen days ,and maintaining at a detectable level up to 25 days.

Objective A program-controlled flexible multi-point temperature measurement device was self developed for collection and analysis of skin temperature signals of diabetic patients and healthy subjects under resting and heating conditions so as to assess vasodilation function of the microcirculation, Methods With reference to the endothelial regulation spectrum of human body, wavelet analysis was performed on skin temperature signals, and the temperature fluctuation amplitudes in diabetic group and healthy control group were compared at different time periods after thermal stimulation. Results The temperature fluctuation amplitude in endothelial spectrum of diabetic group was smaller than that of healthy control group, and the decrease in skin temperature fluctuation after the power-off of thermal stimulation was remarkably smaller than that of control group, indicating that the response to thermal stimulation for diabetic patients was slower. Conclusions Vasodilation function can be quantitatively evaluated by using the fluctuation of skin temperature signals in endothelial spectrum band. Skin temperature monitoring is a potentially easy-implemented method for the health management and early diagnosis of microvascular diseases in diabetic patients.