Although the cesarean section has become an effective means to solve dystocia,high - risk pregnan-cy and other critical obstetrical disease,but the morbidity rate and hospitalization rate of newborn did not decreased obviously because of the continuous increasing of cesarean section rate. Contrary,in recent years,many domestic and foreign researches have found that,compared with normal vaginal delivery babies,cesarean section newborn infants have higher related disease incidence rate after birth,especially respiratory distress sydrome,transient tachypnea of newborn infants,persistent pulmonary hypertension and infection,and more attention should be paid to the fact that cesarean section children are more prone to allergic and immune system diseases as well as neuropsychiatric related diseases. Therefore,this article aims at integrating the researches and providing an overview on recent and long - term effection on newborn infants.

Objective Implement random sample from a simulation population,to evaluate the The impact of samplesize and sample-process on several usual importance evaluate methods,observe the stability of those methods.Methods This study introduced existed importance methods,using PROC SURVEYSELECT procedure to sample a fixed population for 1000 times,generating 1000 same size sample,to evaluate the stability of relative importance methods.We sampled the population to generate datasets with different sample size to observe impact of sample-size on those methods.Results The sum of squared correlation coefficients' estimator is bigger than model R-square,squared standardized regression coefficients' sum is smaller.In contrary,sum of the Product Measure,Relative Weight and Dominance Analysis are extremely close to model R-square.When the sample size small than 1000,the estimator have obviously variation,but the variation decreased when the sample size rise up.Conclusion The dominance analysis has best stability,also has the best match of model R2 in those methods.

OBJECTIVE:To explore the regularity and characteristics of anaphylactoid reactions induced by Magnesium iso-glycyrrhizinate injection,and provide reference for clinical rational drug use. METHODS:27 ADR reports induced by Magnesium isoglycyrrhizinate injection from Hunan Center for ADR Monitoring from Jan. 2014 to Mar. 2015 were retrospectively analyzed. RE-SULTS:In the 27 cases,15 were male (55.56%) and 12 were female (44.44%),with percentage of 1.25∶1;ADR mainly oc-curred in patients with 41-60 years old(51.85%);involved organ/system were mainly skin and its appendages(35.85%)and sys-temic damage(26.42%),the main clinical manifestations were rash,itching,fever,chills,vomiting and dizziness;there were 7 severe ADR reports(25.92%),mainly showed chills,fever and other systemic symptoms;most ADR can be cured(40.74%)or relieved(55.56%)by related processing. CONCLUSIONS:There are no risk tips for severe allergic reactions in instruction,it is necessary to strengthen post-marketing surveillance and further improve the drug instructions to reduce the risk of severe ADR in-duced by Magnesium isoglycyrrhizinate injection.

Objective To express outer membrane protein 2(Omp2) of Chlamydia trachomatis, purify expressed products and study its immunity.Methods The target gene encoding Omp2 167—434 amino acid residues was amplified by PCR from C. trachomatis template DNA. The targeted DNA fragment was cloned into expression vector pET28b(+) and introduced into competent E. coli BL21(DE3) cell. Recombinant Omp2aa_ 167 ～aa_ 434 was expressed after induction by IPTG and analyzed by SDS-PAGE and Western blot, purified with Ni-NTA-His affinity chromatography. The rOmp2aa_ 167 ～aa_ 434 was used to immune rabbits for immunogenicity assessment.Results Restriction enzymes cleavage analysis and DNA sequencing confirmed that the plasmid pET28b(+)/Omp2aa_ 167 ～aa_ 434 was correctly constructed. The 35.0?103 molecular weight pure protein, which specifically reacted with serum from C. trachomatis infected patient by Western blot, was obtained by optimizing the conditions for both expression and purification. The titer of serum antibodies was above 1∶1 280 as detected by ELISA.Conclusion The expressed product showed good immunity.

On the basis of the investigation of the status of doctor-patient relationship in ten cities of China,this article ethically analyzes and studies the situation of trust between doctors and patients,clarifies the lack of trust inevitable and the environmental factors of trust,and puts forward the corresponding proposal.

It is shown by the typical investigation on the status of doctor-patient relationship in ten cities of China that doctor-patient relationship is generally harmony and relative better,and it is affected by many important factors including governmental policy,hospital and staff,and patient aspects,so it is injustice for hospital and staff to be excessively criticized.This article mainly analyzes the factors of hospital and staff and advances the corresponding countermeasure.

The reasons why the relationships between doctors and patients hasn't advanced to a full and true harmonious stage in our country are multiple.From the legal point of view,the research on the important legal issues of doctor-patient relationship is deficient.The law regulating the relationship is hysteretic and even lacking.The system settling the doctor-patient conflict is distempered and imperfect.Both the doctors' and patients' legal knowledge and consciousness are inadequate.Therefore,it is necessary to discuss the legal measures from the four aspects to construct a harmonious medical service system.

Objective:In investigate the status quo of current physician-patient relationship.Method:A self-designed questionnaire is conducted among the medical staff and patients in 40 hospitals(20 grade 3 hospitals and 20 grade 2 hospitals) located in 10 cities of China.Result:9.3% of the total patient responders consider the current physician-patient relationship dissatisfying,with a counterpart ratio of 31.2% in responders of medical staff.Conclusion:Currently in China,main-stream of physician-patient relationship is harmonious,but it can not be denied that medical staff are suffering tremendous stress from the ever tenser physician-patient contradictions.

Various reasons are responsible for the current tension and conflicts in physician-patient relationship,in which media factors contribute a lot.It is of great significance for promoting the construction of a harmonious society and soothing physician-patient conflicts to conduct research on media factors affecting current physician-patient relationship and seek for relevant countermeasures.

y of care,in an effort to build a better doctor-patient relationship.