Paclitaxel is an antimicrotubule agent indicated as first-line therapy for the treatment of advanced carcinoma of the ovary and breast cancer.Oral administration of paclitaxel is preferred as it is convenient to use in hospital and reduces costs.However,due to the low oral bioavailability of paclitaxel,most commercially available for-mulations are injections.This review presents the progress of developed paclitaxel injection and oral formulations to enhance the bioavailability of paclitaxel.

Intestinal mucosal barrier can prevent harmful substance into other tissue,organ and blood across intestinal mucous,and sIgA in feces is an important part of intestinal mucosal barrier.sIgA plays a critical role as an immunological barrier and matters to maintain intestinal homeostasis.SIgA can enhance mucosal barrier[1],protect microbial pathogen from invading and relieve intestinal infammafion symptoms.This review summarizes the research of sIgA in some diseases,to understand its function in the diseases and provide evidence for diagnosis and treatment.

Objective To investigate the expression of vascular endothelial growth factor (VEGF) or matrix metalloproteinase-9( MMP-9) in pancreatic carcinoma( PCA) and its clinical significance.Methods The expression of VECF and MMP-9 in 50 PCA tissues and in 20 normal pancreatic tissues were examined by immunohistochemistry.Results The expression of VECF and MMP-9 was higher in PCA than that in its surrounding parenchyma and normal pancreatic parenchyma( x2 = 2.82,2.96, all P < 0.05 ) ; The expression of VEGF and MMP-9 were related to the tumor differentiation,the clinical stage and the lymphnode metastasis.Conclusion VECF and MMP-9 should play important role in the invasion and metastasis of PCA, and had a certain significance for predicting recurrence and metastasis of pancreatic cancer.

Two cases, prenatal genetic testing for deafness and newborn genetic testing for diseases and talent, were firstly introduced. And then, the author analyzed the ethical issues in these cases, such as question about ge-netic testing technologies, freedom of genetic testing in clinical obstetrics, role of obstetricians in genetic testing, problems about informed consent, and harms to doctor-patient relationship, and so forth. Finally, it put forward some suggestions on commercial genetic testing in obstetric practice.

Objective To develop an ion-pairing reversed-phase high performance liquid chromatography(IP-RP-HPLC)method for the determination of L-methionine sulphoximine(MSX) residues in recombinant protein therapeutics, and to verify and preliminarily apply it, so as to provide a reference for the quality control of related products.Methods A chromatographic analysis method was screened and the mobile phase conditions such as ion-pairing reagent, pH and salt concentration were optimized to develop an IP-RP-HPLC method for the determination of MSX residues in recombinant protein therapeutics. The method was verified for specificity, system applicability, linearity, limit of detection(LOD) and limit of quantitation(LOQ), precision, accuracy and solution stability, by which the residual MSX contents in multiple batches of in-process sample and bulk of recombinant protein therapeutics were determined.Results The chromatographic conditions were determined as follows: using Hypersil GOLDTMC18 column(4. 6 mm × 250 mm, 5 μm) at column temperature of 25 ℃; 50 mmol/L sodium dihydrogen phosphate(containing 5 mmol/L sodium 1-octane sulfonate, pH 2. 0)-acetonitrile(volume fraction of 85 ∶ 15) as mobile phase for isocratic elution at a flow rate of 0. 6 mL/min; detection wavelength of 200 nm; injection volume of 50 μL. The method exhibited good specificity and system applicability. The control substance showed good linearity in the concentration range of 10-100 μg/mL, with a correlation coefficient(R) of 0. 999 95. The LOD and LOQ were 5 and 0. 5 μg/mL, respectively. MSX was not detected in either recombinant protein A or B, and the relative standard deviations(RSDs) of the measured concentrations of six parallel spiked samples were 1. 3% and 1. 1%, respectively, with the recovery rates ranged from 94% to 103%. For recombinant protein A spiked test sample placed at 10 ℃, the RSD of the measured concentrations at 0 h and 12 h were 2. 1%. MSX was not detected in the in-process samples and bulk of recombinant protein A test samples and three batches of recombinant protein B samples.Conclusion The developed IP-RP-HPLC method has the characteristics of simple operation and high sensitivity, and can be used for the quality control of MSX residues in recombinant protein therapeutics.

Although the cesarean section has become an effective means to solve dystocia,high - risk pregnan-cy and other critical obstetrical disease,but the morbidity rate and hospitalization rate of newborn did not decreased obviously because of the continuous increasing of cesarean section rate. Contrary,in recent years,many domestic and foreign researches have found that,compared with normal vaginal delivery babies,cesarean section newborn infants have higher related disease incidence rate after birth,especially respiratory distress sydrome,transient tachypnea of newborn infants,persistent pulmonary hypertension and infection,and more attention should be paid to the fact that cesarean section children are more prone to allergic and immune system diseases as well as neuropsychiatric related diseases. Therefore,this article aims at integrating the researches and providing an overview on recent and long - term effection on newborn infants.

Objective:To observe the efficacy of A clotrimazole combined with ultrasonic atomization of ozone in the treatment of candidal vaginitis. Methods:Totally 200 cases of candidal vaginitis were divided into the observation group and the control group with 100 cases in each. The control group was given A clotrimazole creams through vaginal administration, qd, while the observation group was treated by ultrasonic atomization of ozone additionally, qd, 15min per time with five times. The clinical efficacy and the recurrence rate after 6 months of the two groups were compared. Before the treatment and in the first and fourth week after the treatment, vaginal secretion bacterial culture was performed and pH value was detected. Results:The cure rate and the total effective rate of the observa-tion group was 88% and 98%, respectively, which was significantly higher than that of the control group (69% and 82%, respective-ly, P<0. 05). After the 6-month follow-up, the recurrence rate was 1. 0% in the observation group (1/98), which was significantly lower than that in the control group [7. 3%(6/82),P<0. 05]. After the treatment, the vaginal pH value in the observation group was reduced notably and the number of lactobacillus was increased significantly(P<0. 05), while those in the control group were without significant changes (P>0. 05). Conclusion:A clotrimazole combined with ultrasonic atomization of ozone in the treatment of candidal vaginitis can improve the therapeutic effect.

Objective To discuss the diagnosis value of ultrasonography in hepatorrhexis. Methods The contrast of ultrasonography for 120 cases of hepatorrhexis with operative exploration was made. Results All of the 120 patients were examined with ultrasonography, fragmentation of liver parenchyma in 55 cases, dehiscence of liver parenchyma in 38 cases, liver subcapsular hematoma in 12 cases, and spontaneous rupture of liver cancer in 7 cases. All of the 120 patients were confirmed hepatorrhexis with laparotomy. The positive diagnostic rate of ultrasonography in the hepatorrhexis was 93.3%.Conclusion Ultrasonography is valuable for diagnosing hepatorrhexis and it is one of the important diagnostic approaches.

Objective To investigate the effects of angiotensin Ⅱ type 1 receptor(AT 1R) antagonist,TCV-116,and angiotensin-converting enzyme inhibitory(ACEI),Delapril on neointimal formation after carotid balloon injury in rats. Method TCV-116 and Delapril were administered p.o for 3 weeks(1 weeks before and 2 weeks after carotid balloon injury) in SHR.Neointimal formation of carotid artery was examined.Results Compared with the control group,neointima/media area ratio was significantly reduced(48 3% and 45 5% respectivity,P

Objective To evaluate the clinical significance of serum tumor supplied group of factor (TSGF) in diagnosing colorectal carcinoma. Methods The TSGF concentration in the patients with colorectal carcinoma, with colorectal polyp and healthy subjects were retrospectively reviewed. Results The TSGF concentration in the colorectal carcinoma patients was significantly higher than that in the colorectal polyp patients (P