Objective To explore the effects of herpes simplex virus 2 (HSV-2) latency-associated transcript open reading frame 3 (LAT ORF3) gene on Vero cells against cisplatin-induced apoptosis.Methods Recombinant plasmid enhanced green fluorescent protein-open reading frame 3 (named pEGFP-ORF3) was constructed and transfected into Vero cells; then,reverse transcription (RT)-PCR was performed to detect the expression of the target gene.Cisplatin of 3 mg/L was selected to induce the apoptosis in Vero cells.Cultured Vero cells were transfected with empty plasmid and induced by cisplatin (pEGFP-C2 group),transfected with recombinant plasmid pEGFP-ORF3 and induced by cisplatin (pEGFP-ORF3 group),only induced by cisplatin (cisplatin-induced control group),or remained untreated (normal control group).Subsequently,fluorescence microscopy was conducted to observe apoptotic bodies,Giemsa stain to observe the morphology of cell nuclei,methyl thiazolyl tetrazolium (MTT) assay to evaluate cell proliferation,and flow cytometry to assess cell apoptosis.Data were assessed by using SPSS 13.0 software,and statistical analysis was carried out by one-way ANOVA and t test.Results HSV-2 333 LAT ORF3 gene was successfully cloned.The eukaryotic expression plasmid for LAT ORF3 was constructed,and the expression of LAT ORF3 gene in Vero cells was confirmed by RT-PCR.Giemsa stain showed blue-staining nuclei and pale cytoplasm in recombinant plasmid-transfected and cisplatin-induced Vero cells with a normal shape.The value of cell proliferation (absorbance at 490 nm) by MTT assay was 2.56 ± 0.21 in pEGFP-ORF3 group,similar to that in the normal control group (2.66 ± 0.13,P ＞ 0.05),but significantly higher than cisplatin-induced control group (1.65 ± 0.11,P ＜ 0.05) and pEGFP-C2 group (1.56 ± 0.18,P ＜ 0.05).As far as the apoptosis rate was concerned,no significant difference was observed between pEGFP-ORF3 group and normal control group (4.03％ ± 1.04％ vs.2.13％ ± 0.09％,P ＞ 0.05),but pEGFP-ORF3 group was statistically lower than pEGFP-C2 group (19.45％ ± 2.05％,P ＜ 0.05).Conclusion The transfected HSV-2 LAT ORF3 gene could protect Vero cells from cisplatin-induced apoptosis.

OBJECTIVE: To investigate the survival rate and life expectancy of patients with pneumoconiosis and influence factors in Changsha from 1956 to 2010. METHODS: A total of 3685 patients with pneumoconiosis were diagnosed and reported from 1956 to 2010 in Changsha. The fatality rate and life expectancy were analyzed by life table and the cause of death was analyzed by Kaplan-Meier method and Cox regression model. RESULTS: The death rate increased obviously with age. Age and accumulation death probability showed linearity (Ŷ=1.271+0.041X, r=0.989). The life expectancy was 60.12 years. The first cause of death was pulmonary tuberculosis in patients with pneumoconiosis. Ruling out the influence of pulmonary tuberculosis, pneumoconiosis, and lung source heart disease, the life expectancy of patients with pneumoconiosis averagely extended 0.83, 0.99, and 0.02 years. The death rate of pneumoconiosis-tuberculosis had significant difference with that of the pneumoconiosisnontuberculosis (P<0.01). Cox regression analysis revealed that the main risk factors for the survival of patients with pneumoconiosis included type of work (smashing worker), complication with tuberculosis, type of pneumoconiosis (silicosis). The death hazard ratio or relative risk caused by them was 1.927, 1.749, and 1.609, respectively. CONCLUSION Prevention of pneumoconiosis should focus on smashing workers in Changsha, while its the treatment primarily attaches importance to complication of tuberculosis and lung infection.
Adult ; Aged ; Cause of Death ; China ; epidemiology ; Female ; Humans ; Life Expectancy ; Male ; Middle Aged ; Pneumoconiosis ; complications ; epidemiology ; mortality ; Prevalence ; Proportional Hazards Models ; Risk Factors ; Survival Analysis ; Tuberculosis, Pulmonary ; complications

OBJECTIVE: To investigate the association of the polymorphism of angiotensin-converting enzyme (ACE) gene and pregnancy-induced hypertension (PIH) in Chinese Women. METHODS: We systematically searched CNKI, Wanfang database, VIP and PubMed from database construction to March 2012 to collect case-control studies. Stata 11.0 was used for meta analysis after evaluating the quality of studies and collecting the data. The association was assessed by odds ratio (OR) with 95% confidence intervals (CIs). Publication bias was analyzed by Begg's funnel plot and Egger's regression test. RESULTS: We identified 11 case-control studies on association between ACE gene polymorphism and PIH, which included 806 PIH patients and 900 controls. Overall, significant association was found between ACE gene polymorphism and PIH risk [for D vs I: OR=2.73, 95% CI (1.64, 4.24), P<0.001; for DD+DI vs II: OR=3.11, 95% CI (1.98, 4.90), P<0.001; for DD vs II: OR=5.00, 95% CI (2.30,10.88), P<0.001; for DI vs II: OR=1.97, 95% CI(1.53, 2.53), P<0.001]. CONCLUSION Chinese women with D allele gene deletion have a higher risk of suffering pregnancy induced hypertension syndrome.
Adult ; Alleles ; Female ; Genetic Predisposition to Disease ; Humans ; Hypertension, Pregnancy-Induced ; genetics ; INDEL Mutation ; Peptidyl-Dipeptidase A ; genetics ; Polymorphism, Genetic ; Pregnancy ; Randomized Controlled Trials as Topic ; Risk Factors

OBJECTIVE: To investigate the association of 7 novel genetic loci identified in a recent genome-wide association studies (GWAS) with T2DM in Chinese Dong populations. METHODS: A case-controlled study was performed in individuals of Chinese Dong nationality. The genotypes of PARD3B (rs849230), LOC729993 (rs149228), EPHA4 (rs16862811), HNT (rs3099797), PTPRD (rs17584499 and rs649891), TOMM7 (rs2240727) genes were determined by Multiplex PCR-SNaPshot. The independent association between each polymorphism and T2DM was assessed by unconditional binary logistic regression analysis. The gene-environment interaction was assessed by marginal structural linear odds model. RESULTS: A total of 209 cases of T2DM and 209 control subjects were enrolled in the study. The polymorphism of rs2240727 in TOMM7 gene was associated with T2DM (OR=1.50, per copy of the risk T allele, P=0.004). In addition, CT (OR=2.07, 95% CI: 1.14-3.76) and TT (OR=2.75, 95% CI: 1.46-5.17) were risk genotypes for T2DM. After the correction for multiple test, the association remained significant (all P<0.05). After adjustment for the confounders of age, gender, and BMI, the association remained significant (P<0.05). The results of interaction analysis indicated that there were interaction between rs2240727 locus and BMI, WHR, hypertension and family history of diabetes. After adjustment for age and gender, the results of relative excess risk of interaction (RERI) were 1.5430 (95% CI: 0.5797-2.5062), 2.6520 (95% CI: 1.7516-3.5524), 2.9131 (95% CI: 1.7959-4.0303), 4.2062 (95% CI: 1.1686-8.2439), respectively (all P<0.05). CONCLUSION The rs2240727 genetic variant in TOMM7 is associated with T2DM in Chinese Dong population. There is positive interaction between rs2240727 and BMI, WHR, hypertension as well as family history of diabetes.
Alleles ; Asian Continental Ancestry Group ; genetics ; Case-Control Studies ; China ; Diabetes Mellitus, Type 2 ; genetics ; Gene-Environment Interaction ; Genetic Predisposition to Disease ; Genome-Wide Association Study ; Genotype ; Humans ; Membrane Proteins ; genetics ; Mitochondrial Proteins ; genetics ; Polymorphism, Genetic