Objective To summarize the tending physique of hysteromyoma patients and thus to provide the clinical prevention and treatment methods for the disease.Methods Clinical epidemiology investigation and study methods were adopted to design the unified investigation and study table.Questionnaire survey were performed stochastically to 150 hysteromyoma patients and 150 normal females.Various physique characteristics were recorded.Results Ecchymosis physique were commonly seen in hysteromyoma patients,accounting for 36.7％ in all patients.Conclusion The occurrence of hysteromyoma was closely related to ecchymosis physique.

Objective To explore the clinical efficacy of acupuncture point embedding therapy in the treatment of slow transit constipation (STC)and the influence on the colon transmission function,plasma substance P(SP), vasoactive intestinal peptide(VIP). Methods A total of 128 slow transit constipation patients were involved in the study and divided randomly into two groups by using stochastic indicator: a treatment group (treated with acupuncture point embedding therapy) and a control group (treated with lactulose oral liquid). The clinical effects, colon transmission function, the influence of SP, and VIP were observed for STC patients. Results In the treatment group, CCS score was (13.52±3.93) before treatment and(8.15±3.77) after the treatment. In the control group CCS score was (14.11±4.24) and (9.01±2.93) respectively before and after the treatment, CCS score in both groups showed significantly improvement after the treatment (P<0.01), and there was no significant difference between groups (P>0.05). The plasma SP and VIP level of the two groups after the treatment [treatment group (46.73±1.50) ng/L,(206.10±12.14) ng/L, the control group(40.92±6.25)ng/L,(214.73± 7.62)ng/L] were also improved than before [treatment group was(26.66±0.88)ng/L, (332.68±17.84)ng/L, the control group was (23.32±1.75)ng/L,(306.22±20.01)ng/L], with statistically significant(P<0.05), and the difference between the two groups were also significant(P<0.05).Conclusion The acupuncture point embedding therapy is effective for STC, and it can regulate the level of SP and VIP.

0 05),BP decreased significantly,which was lower in group B than in group A (P

ObjectiveTo observe the effect of Kuiyu dection on ulcerative colitis and to explore its mechanism.Methods120 patients with ulcerative colitis were randomly divided into a treatment group and a control group,60 cases in each group.The treatment group was treated with Kuiyu dection retention enema,1 time a day,15 days for a course of treatment; and the control group was treated with SASP orally,2 g every time,3 times a day,and 15 days for a course of treatment.All patients with a course of treatment for the next 7 days after the rest period of treatment,evaluated after 3 courses.Comprehensive clinical efficacy,the main symptoms,score of integral electronic colonoscopy examination,and the changes of IL-6,IL 8 levels were observed in both groups after treatment with complete remission and six months followed-up,besides,the recurrence rate was also observed.ResultsThe total effective rate was 93.33％ in the treatment group,better than the control group 78.33％ (x2 =9.027,P＜0.05) ; the treatment group also showed better results in diarrhea,pus and blood improvement than the control group (x2=5.630、x2=5.972,P＜0.05),the treatment group could significantly reduce UC Serum IL-6,IL-8 level (x2=8.314,P＜0.01); electronic colonoscopy examination points,hyperemia,erosions,ulcers in both groups were significantly improved,but the treatment group was significantly better than the control group (x2=8.322,P＜0.05) ; 6 months followed-up and the recurrence rate in treatment group were better than control group,and the difference was statistically significant (x2=5.857,P＜0.05).ConclusionThrough decreasing the IL-6,IL 8 levels,Kuiyu dection can treat ulcerative colitis,with significant effect,non-toxic side effects,inexpensive,and easy for use.

Objective To investigate the mechanism of kuiyusan on recovering intestinal mucosa of patients with active ulcerative colitis(UC). Methods Forty-eight patients with active UC were selected as our subjects. They were applied kuiyusan to retention enema for1 time per day for 15 days as a treatment course. Next treatment course were started interval 7 days and all patients were received 3 treatment courses. Changes of pathological signs were observed before and after the treatment with kuiyusan. Results The rate of intestinal mucosal focal small hemorrhages after treatment was 37. 5 %(18 / 48),significantly lower than that at pre-treatment(87. 5%(42 / 48);χ2 = 25. 60,P < 0. 001). The UC mucosal epithelial regeneration rate,disappear rate of intestinal mucosal epithelial goblet cells and Paneth cells regeneration rate at pre-treatment were 18. 8%(9 / 48),31. 2%(15 / 48),6. 3%(3 / 48),significantly different from that after treatment(50. 0%(24 / 48), 6. 3%( 3 / 48 ),20. 8%( 10 / 48 );χ2 = 10. 39,9. 85,4. 36;P < 0. 01 ). The incidence rate of intestinal lymphocyte hyperplasia and infiltration of eosinophils at pre-treatment were 95. 8%(46 / 48)and 100%(48 /48),higher than that after treatment( 72. 9%( 35 / 48 ) and 56. 2%( 27 / 48 )) and the differences were significant(χ2 = 9. 56,26. 88;P < 0. 01). Crypt abscess incidence were decreased from 56%(27 / 48)at pre-treatment to 25%(12 / 48)at after treatment( χ2 = 9. 72,P < 0. 01). Conclusion Kuiyusan can obviously improve small vascular lesions and crypt abscess of the mucous membrane on active UC,reduce neutrophil, eosinophil infiltration,maintain the intestinal epithelial tight junction,improve mucosal barrier,as well as promote healing of mucosal inflammation.

ObjectiveTo evaluate the safety of breast-conserving surgery for ductal carcrnoma in situ (DCIS).MethodsOne hundred and nineteen patients with pathologically confirmed DCIS were analyzed retrospectively. The relationship among local recurrence and distant metastasis with relevant factors including surgical procedure, age, tumor size, estrogen/progesterone status and human epithelial receptor 2 (HER-2) status were analyzed by univariate and multivariate methods.ResultsIn this study 48 patients received breast conserving surgery,71 patients received mastectomy. All patients were followed up for 7-132 months (median 35 months). Chest wall recurrence was found in mastectomy group in one case and none in ipsilateral breast tumor recurrence (IBTR) in breast conservation group (P =0. 384). Two patients had distant metastasis in mastectomy group, and one patient in breast conserving group ( P =0. 383 ). The three year disease-free survival (DFS) were 100％ and 94. 4％ in breast conserving and mastectomy groups respectively(P =0. 225). Univariate analysis showed that age( ＜40 years)was the risk factor for distant metastasis(P =0. 035) ,a factor not confirmed by multivariate analysis (P =0. 086).ConclusionsThere is no significant difference on local recurrence and distant metastasis between breast conserving surgery and mastectomy for DCIS of the breast.

Along with changes of medical model, hospitals need to provide best outcome with lowest cost and best patient′s experience rather than merely medical treatments. It is the cultivation of lean awareness to the doctors that could acquire such outcome. The healthcare lean awareness can be summarized as responsibility awareness, digitalized awareness, detail awareness, and outstanding awareness. Through the cultivation of lean awareness, humanism can immerse into the doctors′practice, which is conducive to train for the great masters of the medicine.

Objective To investigate the effects of propofol on intracellular free Ca2+ concentration and nitric oxide synthase (NOS) activity in PC12 cells exposed to bupivacaine. Methods The PC12 cells were provided by Shanghai Cell Biology Research Institute, Chinese Academy of Sciences and cultured in DMEM liquid culture medium. The cultured PC12 cells were seeded in 36 well plates and randomly assigned to one of 4 groups (n=9 wells each): group Ⅰ control (C);group Ⅱ propofol (P);group Ⅲ bupivacaine (B) and group Ⅳ propofol + bupivacaine (PB). In control group D-Hank solution was added. The cells were exposed to propofol 2 mmol/L and bupivacaine 0.09 mmol/L in group P and B respectively. In group PB the cells were incubated with propofol 2 mmol/L and bupivacaine 0.09 mmol/L simultaneously. After being incubated for 6 and 24 h the apoptosis in BC12 cells was assessed by flow cytometry. Apoptotic rate was calculated. NOS activity and intracellular free Ca2+ coneentration in PC12 cells were determined. Results Bupivacaine significantly increased the apoptotic rate of PC12 cells, the intracellular free Ca2+ concentration and NOS activity in PC12 cells in group B as compared with control group. Propofol significantly decreased the toxic effects of bupivacaine on PC12 cells in group PB compared with group B. Conclusion Bupivacaine is toxic to PC12 cells by increasing apoptosis, intracellular Ca+ concentration and NOS activity in the cells. The toxic effects can be prevented to some extent by concomitant administration of propofol.

Objective To establish a method for determinning quetiapine in human hair by HPLC.Methods A reverse phase HPLC system was performed on Inertsil ODS-C18 column (4.6 mm ×150 mm,5μm)with the mobile phase consisted of 0.01 mol/Lammonioum-methanol(32︰68).The flow rate was 1.0 mL/min,column temperature was 40℃,the detection wavelength was 254 nm.N-hexane was used as extracting solvent.Results The calibration curve was linear in the range of 5-200 ng/mg.for quetiapine.The recovery of extraction >75.0%, the recovery of method was between 95.0% and 105.0%, the intra-day and inter-day precision were all no more than 10.0%.This method met the requirements of biological samples. Conclusion A HPLC method of concentration of quetiapine in human hair is established, which is simple,sensitive,accurate and has a certain value.

Objective To investigate the effects of electroacupuncture (EA) pretreatment on the expression of phosphorylated signal transducer and activator of transcription 3 (pSTAT3) in cortical neurons in a rat model of focal cerebral ischemia/reperfusion (I/R) injury.Methods Forty-five adult male Sprague-Dawley rats,weighing 280-320 g,were randomly divided into 3 groups (n =15 each):sham operation group,I/R group and EA pretreatment group.Focal cerebral ischemia was induced by middle cerebral artery occlusion for 120 min,followed by 24 h of reperfusion.EA of Baihui acupoint lasting 30 min was performed and then the model of focal cerebral I/R was established 24 h later in EA group.Neurological function was assessed and scored at 24 h of reperfusion.The rats were then sacrificed and brains removed for detection of the cerebral infarct volume and expression of pSTAT3 (Ser727) in cortical neurons in ischemic penumbra by immunofluorescence and Western blot.Results Compared with sham operation group,the neurological function score was decreased,the infarct volume was increased,and the expression of pSTAT3 (Ser727) was up-regulated in groups I/R and EA (P ＜ 0.05).Compared with I/R group,neurological function score was increased,the infarct volume was decreased,and the expression of pSTAT3 (Ser727) was up-regulated in group EA (P ＜ 0.05).Conclusion EA pretreatment reduces focal cerebral I/R injury through up-regulating pSTAT3 expression in cortical neurons in rats.