Objective To explore the application effect of home care on the basis of temperament combined with social competence in preschool children with bronchial asthma.Methods A total of 100 preschool children outpatients with bronchial asthma who came to our hospital from January 1 to February 28,2013 were selected with the method of convenience sampling and were randomly divided into observed group and control group with 50 cases in each group.Cases of the observed group were divided into ordinary,middle,and strengthen subgroups according to temperament and social competence.The control group and the ordinary subgroup received routine care interventions,and the other subgroups were given corresponding enhanced care measures.The attack rate of asthma,medical care,quality of life and lung function during one year follow-up were observed and compared between the two groups.Results Within the first six months of follow-up,patients in observed group got better control in acute attacks than the control group [(2.3±0.4) times vs.(3.4±0.3) times,x2=5.710,P＜0.05)].The percentage of forced expiratory volume in the first second and Pediatric Asthma Quality of Life Questionnaire scores in observed group were significantly higher than the control group one years later [(86.9±4.6)％ vs.(85.5±3.8)％ and (90.1±7.5) vs.(76.5±8.0),P＜0.05].Conclusions The application of temperament type combined with social competence showed a good practical value in home care for asthma preschoolers.

Objective To investigate the effectiveness of hyperthermia of different degrees in killing tumor cells and its influence on Na+ -K+ -ATPase activities in erythrocytes. Methods Cultured low differentiated stomach gland tumor cells (SGC-170) and large intestine gland tumor cells (LOVO) (1?106?ml-1 each) were mixed with concentrated RBCs respectively and incubated in warm bath of 37℃ , 42℃, 43℃ , 45℃ or 47℃ for 40 min respectively. After hyperthermic treatment tumor cells were isolated from RBCs using density gradient centrifugation and the live tumor cells were counted by typan staining. The isolated tumor cells were then cultured and the clone formation of tumor cells was checked. The cultured tumor cells were marked with 5-bromo deoxyuridine and DNA metabolism was examined. The Na+ -K+ -ATPase activities in RBC after hyperthermic treatment were also determined. Results The amount of tumor cells was significantly decreased by 40 min hyperthermic treatment in a temperature-dependent manner from 42-471 as compared with the control group (37℃) (P

Antitumor activity of 40% -alcoholic extract from Trachyrhamphus serratus (Temminck et SchlegeDwas assayed by using Heps and EAS tumor models of mice. The results showed the antitumor rate of water -eluted from macroporous absorbing resigns column was 50. 75% on cultured Heps of mice.

Objective To explore how to trigger an HLA Ⅰ restricted CD8 + T cell response to exogenously synthesized peptides in vitro . Methods A new panel of therapeutic peptides based on the immunodominant B and CTL epitopes of HBV PreS 2 region and HBcAg and the tetanus toxoid common T helper epitopes were synthesized by Merrifield solid phase peptide synthesis, and HLA A2 + human PBMCs were used to investigate the immunological properties of the mimetic peptides. Results The results demonstrated that the peptides could trigger vigorous CD8 + HBV specific CTL responses in vitro specifically and effectively. Conclusion The results reveal that T helper plus B epitopes designing with the introduction of short and flexible linker can remarkably improve the immunogenicity of short peptides and hence produce effective CTL responses in vitro .

Objective To evaluate the effect of Ganoderma lucidum on drug resistance of the cisplantin-resistant ovarian cancer cell line(A2780CP).Methods The A2780CP cells were randomly divided into control group and test group.The cells in control group and test group were incubated in culture media alone and in culture media containing Ganoderma lucidum 0.5 mg/ml for 48 h respectively.The resistance index(RI) of A2780CP was determined by WST-1 assay.The expression of Akt,Bcl-2 and p53 protein was measured by Western blot.Results The RI was significantly deeressed,the expression of Akt and Bcl-2 protein was down-regulated,while p53 protein expression was up-regulated in test group as compared with control group(P<0.05).Conclusion Ganoderma lueidum can reduce the resistance of A2780 CP cells to cisplantin by down-regulating the expression of Akt and Bcl-2 and up-regulating p53 expression in A2780CP cells.

Objective To control the incidence of infection during transportation process of supply room,reinforce the management of collecting and sending carts in supply room and ensure the effect of disinfection and safety of use.Methods Available chlorine of 500 mg/L was used to disinfect thein ner face of the clean carts,available chlorine of 1000 mg/L for the outer face,irradiation for 1 hour by ultraviolet lamp before use.The inner and outer faces of the dirty carts were disinfected by available chlorine of 1000 mg/L and irradiation for l hour by ultraviolet lamp.The wheels were flushed by clean water and then sterilized by available chlorine of 1000 mg/L"Clean"or"dirty"was indicated on the carts and they were strictly used and stored.The carts were covered with water-proof cloth,also indicated with"clean"or"dirty".Results The bacteriaJ colony was ≤5 cfu/m2 after disinfection on the clean carts and≤15 cfu/m2 on the dirty carts.Conclusions Disinfection by available chlorine and irradia- tion by ultraviolet lamp can reach the standard of sterile area,clean area and contamination area set by the Ministry of Health.This method is an effective measure in the control of transportation process of supply room with simple,feasible,economical and effective characteristics.

This study was aimed to discuss the ways of converting clinical data entered in the Case Report Form (CRF) to Study Data Tabulation Model (SDTM) database format in clinical research of traditional Chinese medicine (TCM). Currently, there were three approaches for implementing SDTM, which were the pure SDTM approach, submission-only approach and database-only approach. This article compared and analyzed advantages and disadvantages of three approaches and introduced experiences of the Clinical Evaluation Center of China Academy of Chinese Medical Sciences using database-only approach for SDTM implementation. The results showed that pure SDTM approach can maximally embrace SDTM standards. However, the current process and software system should be modified. Therefore, it was time-consuming and expensive. The submission-only approach was the most economic way in the application of SDTM standards. However, the data quality and traceability may not be guaranteed. The database-only approach built the study database based on the SDTM standard by writing transformed program before data entry while the data collection system was not SDTM-compatible. It was concluded that database-only approach for implementation SDTM was a suitable and practical way to TCM clinical research.

Objective To investigate the value of preoperative risk assessments and countermeasures on ultrasound-guided radiofrequency ablation for benign thyroid nodules.Methods The preoperative risk assessments of 311 benign solid thyroid nodules in 274 patients were divided into level 0-Ⅳ,among which 38 nodules on level 0,69 nodules on level Ⅰ,36 nodules on level Ⅱ,59 nodules on level Ⅲ,109 nodules on level Ⅳ.Treatments were performed by moving shot technique or multiplanar shot technique,using Leverage Displacement or Hydrodissection to prevent the adjacent vital structures from thermal injuries.Results 311 thyroid nodules were completely ablated.In ablating the 59 nodules on risk level Ⅲ,thermal injuries were effectively avoided in 30 cases by using Leverage Displacement,while recurrent laryngeal nerve injuries happened in 2 cases using Hydrodissection for 29 nodules.The difference between these two methods on risk level Ⅲ had no statistical significance(P =0.237).To avoid thermal injuries during ablation for 109 nodules on risk level Ⅳ,using Leverage Displacement for 102 nodules,recurrent laryngeal nerve injuries happened in 1 case,while using Hydrodissection for 7 nodules,recurrent laryngeal nerve injuries happened in 2 cases.The difference between the two methods on risk level Ⅳ had statistical significance(P =0.010).No thermal injury happened during ablation for 143 nodules on risk level 0-Ⅱ.Conclusions Preoperative risk assessment on thyroid nodules is helpful in preventing potentially avoidable complications.Proper use of Leverage Displacement can protect adjacent vital structures from thermal injuries during ablation for nodules,which is easy and simple to handle and has a certain practical application value.

To programming one Child's Behavior Problems Computer Screening System (CBPCSS), a series of software for analyzing child behavior individual and group sample. According to the world-famous American ACHENBACH child's behavior checklist, to ensure the screening quality and compatibility of cultures, we revised and standardized the norms of different ages in primary schools and nursery schools in various cities based on the principle of cluster stratified sampling. Then we designed CBPCSS carefully. The system can reliably and rapidly screen an individual child behavior and output the behavior factor curve (appearing in front of the profile). With CBPCSS we can observe the child behavior clearly. It takes twenty times shorter than that of manual screening. On the other hand, CBPCSS has a function of group analysis. The clinical practice proved that CBPCSS could substitute for manual screening completely. It is a powerful tool for social, scientific and pediatric medical workers.

The association between atrial natriuretic peptide (ANP) polymorphism and coronary heart disease (CHD) was studied in Chinese population. The genotypes of ANP T2238C and ANP C-664G were detected by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) methods in 158 consecutive CHD patients and 165 controls. It was found that the distribution of A2A2 genotype in CHD group was significantly higher than that in control group (P<0.05). Stepwise Logistic regression analysis revealed that male, smoking, history of hypertension, history of diabetes, family history of hypertension, high level of serum cholesterol, and ANP T2238C polymorphism were the possible risk factors in patients with CHD (P<0.05). However, there was no significant difference between the patients with CHD and the control group in the distribution of ANP C-664G polymorphism (P>0.05). The results suggest that A2A2 T2238C genotype could be one of the risk factors for CHD (P<0.05, OR: 1.80, 95 % CI: 1.03-3.15).