Objective To evaluate the effects of analytic variations in creatintine measurements on estimated glo -merular filtration rate (eGFR) and the classification of chronic kidney disease ( CKD).Methods A total of 13 157 patients, including inpatients, outpatients and health individual , were enrolled, whose creatinine range from upper reference limit (URL) -12% ×URL to URL +12% ×URL.There were 9 886 males and 3 271 fe-males with an age range of 20 ~89 years.The results of sCr incremented or decremented 4%, 8%, 12% and orig-inal results were divided into 7 groups.The effects of different degree of analytic variation in sCr measurement on eGFR and classification of CKD using eGFR were evaluated .Results When sCr was in the range of URL ±12%×URL, creatintine increased with age, on the contrary, eGFR decreased with age (P <0.05).The mean bias of the results of eGFR compared with the original results of eGFR was increased with the analytic variation of sCr . When the results of sCr had a reverse analytic bias of 12%, the results of eGFR had a forward bias of 16.73%. When sCr results had a forward analytic bias of 12%, the male patients in stage G3 (eGFR in 30 ~59 ml/min/1.73 m 2 ), according to the classification of CKD using eGFR, increased about 20%; while women in this stage could increase by nearly 30%.When sCr results had a reverse analytic bias of 12%, the percentage of female pa-tients in stage G3 could decrease from 38.65% to 10.42%; while male patients could decrease by 7%.Conclu-sion The analytic variation of creatinine change in laboratory testing tolerance range can cause large shift in the distribution of eGFR, which can cause change in the classification of patients .The correct eGFR report relies on the accurate detection of sCr.Routine reporting of eGFR alongside creatintine should pay attention to the detection accuracy of sCr.

A total of 306 fresh human serum samples were randomly selected. Alb concentrations were measured by bromcresol green ( BCG) method, modified bromcresol purple ( mBCP) method, and immunoturbidmetic assay ( ITA) , respectively. GA was measured by an enzymatic method. GA value was expressed as the percentage of GA in the total serum Alb [ GA%(%) =GA/Alb?100%] . When Alb≥40 g/L, the clinical differences between BCG and mBCP, BCG and ITA, and mBCP and ITA were not significant, and there was no statistical difference between GA% BCG and GA% mBCP(P=0. 537); when Alb<40 g/L, BCG had statistical difference between mBCP and ITA ( P<0. 01 ) , and GA% BCG was significantly lower than GA% mBCP ( P<0. 01 ) . No obvious clinical signifi-cance of Alb concentrations was observed measured by BCG, mBCP, and ITA when Alb≥40 g/L. There was no difference in different Alb methods for GA% calculation. When Alb<40 g/L, the consistency of mBCP and ITA was better than that of BCG and ITA, which indicated that mBCP method may be more suitable for the assay of gly-cated albumin value ( GA%) , whereas the GA% may be underestimated if using BCG method for the determination of Alb.

Objective To evaluate the changes of QT dispersion (QTd) after successful percutaneous transluminal coronary angioplasty (PTCA) or stent implantation during the recovery period after acute myocardial infarction (AMI).Methods We studied 57 AMI patients who underwent PTCA or stent implantation and 86 normal coronary artery subjects used as control. Maximal and minimal QT interval (QT max and QT min) and QTd (QTd=QT max-QT min) were measured using 12-lead electrocardiography within 1 d before and 1 h after PTCA and stent implantation or coronary angiography.Results QTd and corrected QT dispersion (QTcd) were significantly prolonged in patients with AMI compared with control group. There was no significant difference in QTd and QTcd between anterior AMI and inferior one. QT max, QTc max, QTd and QTcd after PTCA or stent implantation were significantly decreased.Conclusion QTd and QTcd were significantly decreased after successful PTCA or stent implantation which were performed during the recovery period of AMI, which shows it can produce beneficial effects in decreasing the risk of ventricular arrhythmia and reducing mortality in AMI patients.

It is an urgent task to transform scientific research achievements on traditional Chinese medicine (TCM) into standard for the science and technology workers of TCM. The transformation not only improves the level of TCM standard, but also promotes the scientific research achievement on TCM into productivity. The Medium and Long-Term Strategic Development Plan for TCM Standardization issued by the State Administration of TCM of the People's Republic of China put forward reinforcement of study on method for transformation of TCM scientific research achievement into standard. Thus, this paper presented seven steps for the transformation on the basis of the TCM Standardization Regulation (trial) in order to provide some references.

Objective To explore the anti-hepatic-fibrosis mechanism of Baogan Ning(BN).Methods Fifty Wistar rats were randomized into 5 groups: normal group,model group,high-and low-dosage BN groups(35.4 and 17.7 g/kg respectively),and colchicine group(0.11mg/kg).Rat models of hepatic fibrosis were induced by multiplex factors.Hepatic protein was extracted,and the expression of hepatic leptic receptors of OB-Rb,JAK2 and STAT3 was detected with Western Blot method.Results The protein expression was not obvious in the normal group,and the color of protein band in the medication groups was light but dark in the model group.Compared with the normal group,the expression of OB-Rb,JAK2 and STAT3 increased in the model group.However,the expression of OB-Rb,JAK2 and STAT3 decreased in the medication groups as compared with the model group.And the expression of OB-Rb,JAK2 and STAT3 decreased in the BN groups as compared with the colchicines group.Conclusion The anti-hepatic-fibrosis mechanism of Baogan Ning is probably related with the inhibition of OB-Rb expression,thus inhibit the JAK2/STAT3 message pathway.

Objective:To discuss the mechanism of Baoganning anti-liver fibrosis.Methods:liver fibrosis rat models were established by complex factors.Automatic biochemical analyzer was used to detect TC、TG and RIA isotope analysis was used to detect serum leptin.To observe the influence of Baoganning on TC、TG and leptin level of liver fibrosis model rats. Results:Compared with the normal group,levels of serum TC,TG decreased apparently in model and every drug groups(P0.05).Conclusion:Baoganning can effectively prevent and treat liver fibrosis in rats,which may be closely related to the improvement of liver function,adjustment of lipid and reduction of serum leptin.

Objective: To observe antipyretic, diuresis, anti inflammatory and antisepsis effects of Qinglin Granules. (Herba Polygoni Avicularis, Herba Dianthi, Caulis Aristolochiae Manshuriensis, Semen Plantaginis, Fructus Gardeniae, etc.) Methods: The model of pyretic rats induced by yeast was used to prove antipyretic action. The diuresis action was observed by the test. The anti inflammatory effect was observed by pettitoes swelling in rats and auricle swelling in mice. The paper diffuse methods were used to confirm the antisepsis effect. Results: Qinglin Granules not only had the obvious effect of relieving fever and increasing the amount of urine, but also had the strong inhibition on the two kinds of inflammatory models, moreover it can widen the diameter of the antisepsis ring which showed it had the inhibition on cdibacillus, pseudomonas, aeruginosa, bacillus gasoformans, staphylococcus aureus and gonococcic. Conclusion: Qinglin Granules have the obvious antipyretic, diuresis, anti inflammatory and antisepsis effects.

Objective To investigate the multiparametric magnetic resonance technique in diagnosis of bladder urothelial carcinoma. Methods The clinical data of 80 patients with urinary tract disease who were treat-ed in the Department of Urology in our hospital from October 2016 to March 2017 were analyzed.The patients were divided into two groups:the tumor group(39 patients with bladder cancer)and the control group(41 patients with the remaining bladder disease).The basic information of two groups and pathological diagnosis results were record-ed and analyzed. Multivariate logistic regression analysis was performed to screen out the statistical significance of the diagnosis of bladder cancer. The ROC curve was applied to determine the best diagnostic point. Results The multivariate analysis showed that UBC level(P=0.039),NMP22(P=0.038)and ADC(P=0.028)have signifi-cance in diagnosis of bladder cancer. Pearson analysis showed that there was a significant correlation between the ADC value and the NMP22(r = 0.333,P = 0.038). The ROC curve shows that the ADC area under the ROC curve was 0.750. The diagnostic sensitivity of ADC value was 0.9750 × 10-3mm2/s;The diagnostic sensitivity was 73.2% and specificity was 69.2%. Conclusion Multiparametric magnetic resonance imaging is of great value in the diagnosis of bladder cancer.

BACKGROUND AND PURPOSE: Encephalitis caused by Listeria monocytogenes (L. monocytogenes) is rare but sometimes fatal. Early diagnosis is difficult using routine cerebrospinal fluid (CSF) tests, while next-generation sequencing (NGS) is increasingly being used for the detection and characterization of pathogens. METHODS: This study set up and applied unbiased NGS to detect L. monocytogenes in CSF collected from three cases of clinically suspected listeria meningoencephalitis. RESULTS: Three cases of patients with acute/subacute meningoencephalitis are reported. Magnetic resonance imaging and blood cultures led to a suspected diagnosis of L. monocytogenes, while the CSF cultures were negative. Unbiased NGS of CSF identified and sequenced reads corresponding to L. monocytogenes in all three cases. CONCLUSIONS: This is the first report highlighting the feasibility of applying NGS of CSF as a diagnostic method for central nervous system (CNS) L. monocytogenes infection. Routine application of this technology in clinical microbiology will significantly improve diagnostic methods for CNS infectious diseases.
Central Nervous System ; Cerebrospinal Fluid ; Communicable Diseases ; Diagnosis ; Early Diagnosis ; Encephalitis ; Humans ; Listeria monocytogenes* ; Listeria* ; Magnetic Resonance Imaging ; Meningitis, Listeria ; Meningoencephalitis* ; Methods