AIM: To investigate the extraction of anti-thrombotic contituent from Earthworm ( Pheretima). METHODS: The prothrombin time (PT)of rabbit blood and antithrombin of 14 samples that were extracted by different methods were determined in vitro. RESULTS: The sample that was percolated with 70% alcohol and the sample that was extracted by semi-bionic method were better than ones that were extracted by other methods. CONCLUSION: It is a simple and practicable method to percolate Earthworm with 70% alcohol.

Objective To investigate the invasion ability and collagenolytic activity of human glioma cells in vitro.Methods Boyden chamber invasion assay was employed to evaluate the cell migration ability of human malignant glioma cell line U87MG in vitro and the effect of conditioned medium of U87MG cells on matrix collagenolytic activity was tested by agar-gelatin gel.Results The number of U87MG glioma cells migrating through the Matrigel-coated membrane was more than that of addition of EDTA or anti-MMP-2 antibody in U87MG glioma cells(P0.05).EDTA or anti-MMP-2 antibody markedly inhibited the number of the migrated cells,the inhibitory rates were 79.2% and 77.1%,respectively. The conditioned medium collected from the U87MG cells showed an increase in the transparent ring area on agar-gelatin gel.Inhibition of MMP-2 enzymatic activity by EDTA or anti-MMP-2 antibody reduced the transparent ring area on agar-gelatin gel.Conclusion Both invasion ability and collagenolytic activity of U87MG cells are depended on MMP-2,suggesting that MMP-2 plays an important role in glioma invasiveness.

Objective To investigate the relationship between the expression of estrogen receptor (ER),progesterone receptor(PR)and epidermal growth factor receptor(EGFR)in uterine leiomyoma and the recurrence after myomectomy.Methods Eighty cases underwent myomectomy were enrolled in this study.ER,PR and EGFR expression in uterine leiomyoma tissue and normal myometrium tissue were examined by immunohistochemical technique.All the patients were followed up regularly after myomectomy.Results The positive expression rate of ER,PR and EGFR in uterine leiomyoma tissue were significantly higher than those in normal myometrium tissue[88.8％(71/80)vs.48.8％(39/80),92.5％(74/80)vs.30.0％ (24/80),86.2％(69/80)vs.57.5％(46/80),P ＜ 0.05].The recurrence rates in ER,PR and EGFR positive expression patients were significantly higher than those in negative expression patients[42.3％(30/71)vs.22.2％(2/9),41.9％(31/74)vs.16.7％(1/6),42.0％(29/69)vs.27.3％(3/11),P ＜ 0.05].The recurrence times in ER,PR and EGFR positive expression patients were significantly shorter than those in negative expression patients(P ＜0.05).The expression of EGFR had positive correlation with ER and PR(r =0.837,0.702,P ＜ 0.05).Conclusions The recurrence of uterine leiomyoma may have correlation with high expression of ER,PR and EGFR in uterine leiomyoma.

Objective To discuss the position nursing for neonates with type three congenital esophageal atresia after operation. Methods We selected 10 patients with type three congenital esophageal atresia from September 2004 to May 2007.The respirator, trachea catheter was removed after operation and the SpO2 was lower than 95% through head mask.Then the SpO2 was compared in different positions of patients,that is face forward,face lateral, face forward with hypsokinesis,face lateral with hypsokinesis. Results All the ten patients survived the operation.The Sp02 in the four positions under oxygen inhalation were (90.30±0.95)%, (89.90±0.74)%, (96.80±1.48)%, (95.90±0.88)%.The former two results were statistically different from the latter two results (P ＜ 0.01). Conclusions The position of face forward with hypsokinesis and face lateral with hypsokinesis facilitated the transition to natural respiration for patients with type three congenital esophageal atresia after remove of respirator and trachea catheter.This could prevent the incidence of complications and the probability of reuse of respirator.

BACKGROUND: Transforming growth factor beta 1 (TGF-β1) is a first selected growth factor in study of articular cartilage tissue engineering. Suitable concentration can stimulate proliferation, division and differentiation of articular chondrocytes. OBJECTIVE: To establish an special inducing system for differentiation of bone marrow mesenchymal stem cells (BMSCs) into chondrocytes in contained TGF-β1 culture in vivo,and to observe the changes in cell form and phenotype.METHODS: BMSCs were separated and purified from rabbit tibial tubercle using attachment culture method. Surface antigen of the third-generated BMSCs was determined by using flow cytometry. Subsequently,the third-generated BMSCs were induced with TGF-β1-contained special inducing system in 21-day culture, which was compared with human septal cartilage cells of nose following induction. Collagen type Ⅱ was qualitatively determined using immunohistochemical method.RESULTS AND CONCLUSION: Rabbit BMSCs were separated and purified using attachment culture method. The third-generated BMSCs were positive for surface antigen CD44,but negative for surface antigen CD34 and CD45. Cell form was irregular 21 days after induction. Immunohistochemical staining for type II collagen demonstrated positive cells. Results suggested that under culture system containing TGF-β1 BMSCs may directionally differentiate into chondrocytes,and there were not significant differences with normal chondrocytes.

The paper introduced the organization culture building of a private hospital, in creating the Aikang hospital as your home culture and Aikang values for building a harmonious workplace for the hospital. The management system reform features the separation between regulations and management,building of the supporting system and operating system, for better quality of care with advanced management practice. The social rewards feature great efforts in supporting the disadvantageous population, and undertaking public health service and charity activities. These care and love to the community help the hospital to fulfill its social responsibilities.

Objective To explore the application value of hysteroscopy in recurrent implantation failure ,and discuss what was the best time to transfer embryos after the operation .Methods The patients who had undergone two or more failed IVF-ET cycles were separated into two groups .The patients of group A ( n=210) received hysteroscopy , and the patients of group B ( n=71) did not receive hysteroscopy evaluation .Intrauterine lesions diagnosis were trea-ted during the procedure .Patients without intrauterine pathologies were given slight curettage .The recurrent IVF-ET were performed after the procedure .Then we analyzed the clinical pregnancy rate of the two groups and determined the best time to transfer embryos after the operation.Results In group A,57 patients were pregnant.In group B, 8 patients were pregnant .There was a significant difference in the clinical pregnancy rates between the two groups (χ2 =7.52,P<0.01).In the pregnant patients of group A ,about 94.7%were pregnant in 6 months after hysterosco-py.Conclusion The patients with recurrent IVF -ET transfer failure should be evaluated prior to commencing IVF-ET cycle to improve the clinical pregnancy rate .It is better to transfer embryos within 6 months after hysteroscopy operation.

SF-36 scale has been widely accepted because of its objectivity and scientificity. However, until now its application in TCM treatment for rheumatoid arthritis is not common. This article discussed the significance of SF-36 scale as the main content of life quality assessment in the efficacy evaluation system of TCM treatment for rheumatoid arthritis, which can provide certain references for the promotion of the application in this field.

Objective:To explore the relationship of life events and internet use in junior high school students.Methods:123 7th grade students were asked to complete the Chinese Adolescent Life Events Checklist,Internet Service Preference Questionnaire for Adolescence and Adolescent Pathological Internet Use Scale.Results:Students' life events scores were lower than the norm significantly;Junior high school students' life events positively correlate with pathological internet use,as well as with salience,tolerance,compulsion symptoms,social comfort,and negative outcomes subscales;Internet service preference closely correlates with pathological internet use;Subjective stress,social preference of internet can predict pathological internet use significantly.Conclusion:Pathological internet use is closely related to life events and social service.

The recent studies have shown a significant relationship between persistent organichorine pollutants(POCPs) and semen quality. The biological characters and source of POCPs, the effect mechanisms of POCPs on semen quality, sperm structure and function were reviewed in this paper. The epidemiological evidences for the reproductive disturbance of men caused by POCPs were discussed and the research direction in the future was pointed out.