BACKGROUND:There are some poisonous and hazard materials released from decoration materials and office furniture that will deteriorate the air of office if the ventilation of air-condition system is not very good. OBJECTIVE:To investigate the effects of newly decorated air-condition office to human health. DESIGN:A retrospective observational comparative study taking the office staff in newly decorated air-conditioned environment as the subjects and the office staff in natural ventilation environment with same decoration as the controls. SETTINGS:Occupational medicine department of a disease control and prevention center of a city. PARTICIPANTS:This study was conducted in the Department of Occupational Medicine of Dalian Centre for Disease Control and Prevention from April to May 2001.Inclusive criteria:office staff in a newly decorated and airconditioned telecommunication building of either sex;Exclusive criteria:people with acute or chronic disease history.Twenty-three office staff working in the airconditioned office was chosen as the observational group,the ratio of male to female was 12 to 11,aged from 24 to 51 with the mean age of 33.1 years old,average length of service of 14.2 years and average working length in air-conditioned office of 6.8 months.Other 23 office staff was randomly selected from natural ventilation office in the same building with the same decoration condition,the ratio of male to female was 10 to 13,aged from 22 to 58 with mean age of 36 years old, average length of service of 16.7 years and working length in this office for 8.2 months. INTERVENTIONS:To monitor the volatile organic compounds,hazard substance and microclimate in the decorated office with air-conditioner and conduct investigation to the health status of staff. Subjective symptoms between staff in different offices. RESULTS:The concentration of formaldehyde and ammonia exceeded the national hygienic standards in both air-conditioner and naturally ventilation offices,in which formaldehyde exceeded 6.1 and 2.3 times respectively while ammonia exceeded 8.5 and 2.3 times respectively.The illumination,assessment index of positive and negative ions and total bacteria in office with air-conditioner were lower than those of natural ventilation office (P< 0.01).The symptoms manifested in staff working in air-conditioned office such as dizziness,headache,chest pain,dry eye,decreased vision and menoxenia were more distinct than control group(P< 0.01).Dry mouth and hair losing were also more popular in air-conditioned group(P< 0.05). CONCLUSION:More attention should be paid to choosing environmental friendly building and decoration materials and office furniture when decorating.It is very important to make sure the ventilation of air-conditioner is normal and there is enough air change flow.

Objective To improve the treatment level of serous otitis media(SOM)in children.Methods 56 patients were treated by positive pressure tympanic administration of ?-Chymotrypsin and Triamcinolone Acetonide through auripuncture into a single hole and 55 cases by myringotomy with grommet insertion.Results The total effective rate of serous otitis media by positive pressure tympanic administration through auripuncture into a single hole and by myringotomy with grommet insertion was 89.8%,90.8% respectively.But the former obviously had more advantages over the latter.Conclusion The technique is better in that it is safe,painless,economical,and non-traumatic.It is an effective method in treating serous otitis media in children.

BACKGROUND: Transforming growth factor beta 1 (TGF-β1) is a first selected growth factor in study of articular cartilage tissue engineering. Suitable concentration can stimulate proliferation, division and differentiation of articular chondrocytes. OBJECTIVE: To establish an special inducing system for differentiation of bone marrow mesenchymal stem cells (BMSCs) into chondrocytes in contained TGF-β1 culture in vivo,and to observe the changes in cell form and phenotype.METHODS: BMSCs were separated and purified from rabbit tibial tubercle using attachment culture method. Surface antigen of the third-generated BMSCs was determined by using flow cytometry. Subsequently,the third-generated BMSCs were induced with TGF-β1-contained special inducing system in 21-day culture, which was compared with human septal cartilage cells of nose following induction. Collagen type Ⅱ was qualitatively determined using immunohistochemical method.RESULTS AND CONCLUSION: Rabbit BMSCs were separated and purified using attachment culture method. The third-generated BMSCs were positive for surface antigen CD44,but negative for surface antigen CD34 and CD45. Cell form was irregular 21 days after induction. Immunohistochemical staining for type II collagen demonstrated positive cells. Results suggested that under culture system containing TGF-β1 BMSCs may directionally differentiate into chondrocytes,and there were not significant differences with normal chondrocytes.

BACKGROUND: Embryonic stem cells or bone marrow mesenchymal stem cells possess potential to treat cartilage defects, but the practicability is restricted by many factors. Therefore, it is significant to explore a new source of cells.OBJECTIVE: To investigate the feasibility of differentiating human umbilical cord blood stem cells into chondrocytes. METHODS: Human mesenchymal stem cells was obtained from term delivered umbilical vein, labeled by BrdU, and co-cultured with rabbit chondrocytes using Transwell method. The harvested cells were identified by immunocytochemistrical analysis. RESULTS AND CONCLUSION: The human umbilical cord blood stem cells were differentiated into chondrocytes under induction of rabbit chondrocytes, cytoplasm of which were stained into purple, and cellular nucleus was yellow stained with BrdU. The results revealed that human umbilical cord blood stem cells can in vitro differentiate into chondrocytes.

Adolescent ; Adult ; Aged ; Bloodletting ; Child ; Child, Preschool ; Combined Modality Therapy ; Dermatitis ; therapy ; Female ; Humans ; Infant ; Insect Bites and Stings ; therapy ; Lymphangitis ; therapy ; Male ; Middle Aged ; Moxibustion ; Young Adult

Objective To explore the effect of employee assistance program on male nurses' professional values and professional identity.Methods Fifty male nurses participated in the program by group counseling and individual counseling.The professional values scale (NPVS-R) and the occupational identity scale were used to investigate the changes in their professional values and occupational identity before and after the intervention.Result After intervention,the occupational values and occupational identity of male nurses were significantly better than before intervention (P＜0.01).Conclusion The employee assistance program for male nurses can promote the formation of positive professional values and professional identity sense.

目的比较国产重组链激酶与尿激酶静脉溶栓治疗老年人急性心肌梗死(AMI)的临床疗效。方法97例60岁以上AMI患者随机分为链激酶组(54例)和尿激酶组(43例)进行静脉溶栓治疗 ,比较两组患者的疗效及不良反应。结果冠状动脉血管开通率链激酶组75.9%,尿激酶组55.8%;并发症中,血痰:链激酶组5.6%,尿激酶组4.7%;血尿:链激酶组1.9%,尿激酶组2.3%;局部出血:链激酶组7.4%,尿激酶组4.7%;低血压:链激酶组3.7%,尿激酶组4.7%;过敏反应:链激酶组1.9%,尿激酶组2.3%;5周预后两组患者无显著性差异。结论国产链激酶静脉溶栓治疗老年人急性心肌梗死的冠状动脉血管开通率较尿激酶高,不良反应发生率与后者无显著性差异,可作为老年急性心肌梗死患者静脉溶栓治疗的首选药物。

Objective To study the diagnostic and distinguishing diagnostic value in primary hepatic carcinoma (PHC)and metastatic hepatic carcinoma (MHC)by the serum sialic acid (SA)detection.Methods During January 2012 to June 2014, 100 cases of patients with PHC,91 cases of patients with MHC,155 cases of benign liver disease patients,and 139 healthy people in Wuyi Traditional Chinese Medicine Hospital were included into the study.The concentration of serum SA and AFP were detected by chemical enzymatic method and chemiluminescence method,SPSS1 9.0 was used to analysis the results.Re-sults The concentration of serum SA in PHC patients (701.08±189.33 mg/L)were significantly higher than benign liver disease patients (588.38±98.51 mg/L)and healthy people (572.37±89.13 mg/L),there was statistical significance (P=0.000),the significantly statistical differences were also in MHC (790.20±162.29 mg/L)and PHC patients (P=0.027). Serum SA in the diagnosis of MHC sensitivity,specificity and AUC were 84.6%,85.2% and 0.895,compared with serum AFP (sensitivity 22.2%,specificity 29.6% and AUC 0.301)had statistically significance (P=0.000).Conclusion The se-rum SA has important clinical significance in the diagnosis and distinguishing diagnosis of PHC and MHC.

Objective To investigate the correlation between Fas-670 gene polymorphisms and the clinic pathologic index of breast cancer in Qinghai district .Methods The polymorphisms of Fas-670 gene were detected by polymerase chain reaction-restric-tion fragment length polymorphisms assay (PCR-RFLP) in 232 female breast cancer patients ,χ2 test was applied to assess the asso-ciation between the polymorphisms of Fas-670 gene and breast cancer clinic pathologic index .Results In 232 breast cancer pa-tients ,the frequency of Fas-670 homozygous wild type(AA) ,heterozygous type(AG) ,homozygous mutant type(GG) were 39 .7%(92/232) ,48 .7% (113/232) ,11 .6% (27/232) respectively .It was no statistical significance between Fas-670 gene polymorphisms and the clinic pathologic index of breast cancer in Qinghai district(P>0 .05) .Conclusion The polymorphisms of Fas-670 gene is not associated with clinic pathologic index of breast cancer in Qinghai district .

OBJECTIVE:To evaluate the rational use of antimicrobials in 160 cases undergoing orthopaedic operations.MET_HODS:To analyse and evaluate the use of antimicrobials in 160 cases undergoing orthopaedic operations in 1999 in respect to the cost of antimicrobials related cost ratio,usage,clinical efficacy,and adverse drug reactions.RESULTS:The cost of antimicrobials per person()was 1 676.55 yuans and average day of treatment()was (7.3?3.4)d.151 patients healed by first intension.9 patients recovered in delayed healing.The ratio of the total cost,cost of drugs and cost of antimicrobials was 1∶0.38∶0.14.CONCLUSION:The use of the antimicrobials in 160 cases undergoing orthopaedic operations was reasonable.