Objective To evaluate the outcomes of microdiscectomy for treatment of recurrent lumbar disc hemiations. Methods There were 18 cases of recurrent lumbar disc berniations accepted microdiscectomy (reoperation group), compared with 30 cases of primary lumbar microdiscectomy (primary group). Operating time, blood loss and length of hospital stay were compared between two groups. MacNab criteria were used to evaluate the outcomes. Results Operating time was a mean of 75 min in reoperation group, 52 min in primary group. Blood loss of reoperation group was 70 ml, 50ml in primary group. Length of hospital stay was 8.5d in reoperation group, 6.5d in primary group. There were one dural tear, two Ls root lesions and 5 more numbness reoperation group, but recovery all patients. There were one dural tear in primary group. 17 cases got follow-up mean 12.1 months in reoperation group and 29 cases in primary group mean 11.8 months. The results demonstrated 88.2% success rate in reoperation group and 93.1% in primary group. There was no significant dif-ference in two groups (P ＞ 0.05). Conclusion Outcomes of Lambar mierodisceetomy with mini-open for treatment of recurrent lumbar disc herniations are similar to primary lumbar mierodiscectomy.

Objective: To evaluate the effectiveness of family interventions among children with allergic rhinitis receiving immunotherapy, so as to provide insights into improvements of allergic rhinitis treatment. Methods: A total of 80 children with allergic rhinitis admitted to The First People's Hospital of Linping District from July 2018 to July 2021 were enrolled and randomly assigned into the control and intervention groups, of 40 participants in each group, and all children underwent immunotherapy. Children in the control group received routine interventions, while participants in the intervention group were given family interventions for 3 months, including health education, psychological counseling, and periodical follow-up. Parental awareness of allergic rhinitis was investigated using self-designed questionnaires, and the compliance to immunotherapy was evaluated. The clinical symptoms were evaluated using the symptom scores and the Rhinoconjunctivitis Quality of Life Questionnaire (RQLQ), and the prognosis was evaluated using the Sino-Nasal Outcome Test-20 (SNOT-20). Results: There were no significant differences between the intervention and control groups in terms of gender, age, course of disease or disease severity (P>0.05). The scores for parental awareness of etiology [(9.56±0.25) vs. (7.45±0.85)], inducement factor [(8.84±0.62) vs. (6.76±1.36)], medication management [(9.56±0.25) vs. (7.97±0.85)] and daily life management of allergic rhinitis [(9.14±0.55) vs. (8.14±0.46)] were significantly higher in the intervention group than in the control group (P<0.05). The proportion of participants' compliance to immunotherapy was significantly higher in the intervention group than in the control group (92.50% vs. 75.00%, P<0.05). The scores for clinical symptoms [(3.12±0.94) vs. (3.96±1.23)], RQLQ score [(3.31±0.87) vs. (3.87±1.02)] and the SNOT-20 scores for nasal symptoms [(6.54±2.14) vs. (8.22±2.45)], sleep disorders [(4.11±0.58) vs. (5.24±1.03)], associated symptoms [(5.29±1.52) vs. (6.34±2.01)] and emotional consequences [(7.52±1.85) vs. (9.19±2.69)] were significantly lower in the intervention group than in the control group post-interventions (P<0.05). Conclusion Family interventions are effective to improve the compliance to immunotherapy, clinical symptoms, prognosis and quality of life among children with allergic rhinitis

Objective To evaluate the susceptibilities of different testing methods of tigecycline against IRAB. Methods MIC and inhibitory zone diameters of 32 strains of IRAB for tigecyclinc were determined by different testing methods.Data were analyzed by comparing the results from each method to those produced by the reference macro broth dilution method. Results The rates of sensitivity intermediate resistance detected by macro broth dilution method , agardilution , MTS and Vitek-2 instrument , disk diffusion method were as follows , 34.375%, 65.625%, 0%, 3.125%, 81.25%, 15.625%, 75%, 25%, 0%, 78.125%, 21.875%, 0%, 3.125%, 81.25%, 15.625%. Compared with macro broth dilution method ,the key major error rate of other methods was 0% by FDA breakpoints , but the CA and EA rates were less than 90%. Conclusions Tigecycline has good activity against the IRAB, with significant difference in susceptible rate among the different methods.

Objective To develop new methods to cultivate, retrieve and purify oligodendrocyte progenitor cells (OPCs). Methods Primary OPCs were isolated from mixed glial cultures of newborn Sprague Dawley rat forebrains by using a selective detachment procedure, further purified by differential adhesion, and maintained in a chemical conditioned medium. The characteristic of OPCs was determined by both electrophysiological recording and immunostaining. Results Immunocytochemical analysis revealed that more than 90% of the isolated cells expressed the OPCs-specific marker NG2. OPCs exhibited a moderate input resistance, delayed rectifier K+ currents (IK) that could be antagonized partly by TEA-Cl and CsCl, small tetrodotoxin (TTX) sensitive Na+ currents that failed to generate typical action potentials. Conclusion Isolation and purification by selective detachment and differential adhesion procedure are effective to obtain highly purified OPCs. OPCs, distinct from neuron, astrocyte, oligodendrocyte or microglia, possess unique physiological properties.

Objective To observe the postnatal development and perinatal electrophysiological characteristics of oligodendrocyte precursor cell (OPC) in rats. Methods Immunohistochemistry and Western blot were applied to determine the expression of NG2 OPC in cerebral cortex and hippocampus at various developmental stages of SD rats. Electrophysiological characteristics of OPC were also recorded in slices of 7-day rats. Results The majority of hippocampal and cerebral OPC exhibited stellate shape,a small cell body with a few processus. Total population of the NG2 immunopositive OPC was numerous at P7d in cerebral cortex and hippocampus. OPC expressed in adult rats with slightly more quantity. Moreover,OPC in hippocampus of P7d rats typically exhibited small inward sodium current and weak active responses,whereas only outward potassium current and inactive responses were recorded in white matter OPC of P7d rats. Conclusion Total population of OPC and relative optical density of NG2 are the highest in P7d rats at the postnatal developmental stages. OPC in cerebrum and hippocampus of P7d rats displays electrophysiological heterogeneity.

[Objective]To report the initial outcomes of occipitocervical fixation using plate-rod-polyaxial screw system and investigate whether this new system offers any advantage over other existing methods of fixation.[Method]Fourteen cases(male 9,female 5)accepted cervicooccipital fixation with plate-rod-polyaxial screw system.There were 3 cases of odontoid dysplasia with dislocation;spinal canal tumor in 3;2 cases of metastatic carcinoma with pathologic fracture of C_1 and C_2,rheumatoid arthritis with cervicooccipital instability,and fasilar impression;burst fracture of C_1 with oecipital-atlantal-axial instability in 1.Twelve cases had neck pain.Thirteen cases had neurological deficits in different degrees.JOA score was 12.4 on average.[Result]The positions of all screws were good.There was no complication related to inserting screws.All neck pain released.Fourteen-months follow-up was obtained on average in 13 patients.Cervicooccipital nonunion was in one case and its occipital screws were pulled out.Twelve patients had satisfactory fixation and got firm fusion.Ameliotate rate of JOA score of the neurological function was 63.0%.[Conclusion]These initial data indicate that posterior occipitocervical fixation using plate-rod-polyaxial screw system is safe and reliable,and satisfactory clinical outcome can be obtained.The system appears to offer some advantages over other existing methods of fixation.

Objective To test the influence of Lactobacillus rhamnosus GG (LGG) in down-regulating the IL-8 production in Caco-2 cells induced by TNF-?. Methods Caco-2 cells were treated with living LGG (1?106 CFU/L) with or without the presence of TNF-? in the culture media. The production of IL-8 was measured by quantitative ELISA. Results TNF-? significantly increased IL-8 secretion by Caco-2 cells at 24 h and 48 h of incubation as compared with the control group (5.33 ng/mg vs 7.36 ng/mg, 15.69 ng/mg vs 32.29 ng/mg, P

Objective:To study the effects of periodontal treatment on the levels of serum lipids and lipoprotein associated phospho-lipase A2(LP-PLA2)in patients with chronic periodontitis(CP)and metabolic syndrome(MS).Methods:52 cases of CP with MS were included,the blood lipid levels,clinical periodontal indexes and white blood cell count(WBC)were detected before and 3 months after treatment.Results:Before and after periodontal therapy the levels of AL(mm)were 5.02 ±0.68 and 3.61 ±0.43(P<0.01),PD(mm)4.07 ±0.46 and 2.52 ±0.39(P<0.01),BOP positive loci(%)92.13 ±6.98 and 37.41 ±8.19(P<0.01), PLI 1.38 ±0.29 and 0.89 ±0.27(P<0.05),TG(mmol/L)1.99 ±0.42 and 1.45 ±0.32(P<0.01)and TC(mmol/L)6.11 ± 0.38 and 5.17 ±0.41(P<0.01),HDL(mmol/L)1.06 ±0.22 and 1.41 ±0.19(P<0.05),respectively.Before and after treat-ment WBCs(×103/L)were 6.03 ±0.42 and 5.52 ±0.37(P<0.01),serum LP-PLA2(mg/L)31.02 ±9.81 and 23.89 ±14.15 (P<0.01),respectively.Conclusion:Periodontal therapy can improve the blood lipid levels in patients with CP and MS.

SF-36 scale has been widely accepted because of its objectivity and scientificity. However, until now its application in TCM treatment for rheumatoid arthritis is not common. This article discussed the significance of SF-36 scale as the main content of life quality assessment in the efficacy evaluation system of TCM treatment for rheumatoid arthritis, which can provide certain references for the promotion of the application in this field.

Objective To discuss the influence of respiratory training and prompting effective discharging of sputum for chronic obstructive pulmonary disease (COPD) patients in acute aggravation period. Methods 50 patients were randomized into the intervention group and the control group with 25 cases in each group.The intervention group received respiratory training and prompting effective discharging of sputum while the control was only given routine treatment and nursing.The ratio of first second expiration volume to forced vital capacity, oxygen partial pressure (PaO2),carben dioxide partial pressure (PaCO2) ,blood oxygen saturation (SpO2)and the in-hospital days were compared between the two groups. Results The clinical effect in the intervention group was superior to that of the control group with shorter in-hospital days (P<0.01).Conclusion Better results could be obtained by application of respiratory training and prompting effective discharging of sputum for COPD patients in acute aggravation period.