[Objective] To observe the therapeutic effect of pressing- moxibustion on Baihui (DU20) point combined with electroacupuncture (EA) on cervical spondylosis (CS) of vertebral artery type. [Methods] A single - blind random - controll trial was carried out in 34 cases of CS of vertebral artery type. The cases were randomized into two groups: the treatment group (Group A, n = 17) treated with pressing- moxibustion on Baihui point and EA on bilateral Fengchi (GB20) point and Jiaji (EX - B2) point of impaired vertebrae and the control group (Gropup B, n = 17) with EA alone. Plasma contents of thromboxane 82 (TXB2), 6-keto-prostaglandin Fl alpha (6-keto-PGF1?) and their ratio were measured before and after treatment. [Results] In the treatment group, 9 were cured, 6 markedly effective, 2 effective and 0 ineffective and 4,6,5 and 2 in the control group respectively; the markedly effective rate was 88.2% which was superior to that (58.8%) in the control group (P

Objective To explore the effect of bellybutton exposure on premature navel nursing effect.Method Three hundred and ninety-three premature infants were randomly divided into experiment group(n=195)and control group(198). Bellybutton fasciation and bellybutton exposure were used in the control group and the experiment group respectively. The two groups were compared in terms of umbilical shedding,time of umbilical healing,umbilical swelling,bleeding and breathing.Result The umbilical healing time in the experiment group was significantly shorter than that of the control group(P<0.05). The incidences of omphalitis,umbilical swelling and bleeding, changed breathing in the experiment group were significantly lower than those of the control group(all P<0.05).Conclusion The bellybutton exposure is effective in promoting the umbilical shedding in premature infants and reducing the incidence rate of premature navel infection.

Objective To investigate a method to detect the sentinel lymph nodes(SN) of thyroid papillary carcinoma and its predictive value for cervical metastasis of carcinoma. Methods Intraoperative methylene blue dye mapping was performed in 24 cases of thyroid papillary carcinoma. The coincidence rate of frozen section pathology and routine section pathology of SN was observed,and the predictive value of SN for (metastasis) of the cervical lymph nodes was noted. Results SN was successfully detected in 21(87.5%) of 24 cases. The average number of SN was 3 nodes. There was one false negative case, the false negative rate was 4.8%(1/24), and no false positive cases were found. The predictive value of sentinel lymph nodes to (cervical) lymph node metastasis was 83.3%.Conclusions Methylene blue staining to identify sentinel lymph nodes could accurately predict the status of cervical lymph node metastasis of thyroid papillary carcinoma.

Objective To observe the impact of ningxinjieyu capsule on contents of monoamine neurotransmitters and their metabolites in cerebral cortex of chronic depression model rats.Methods The chronic stressed depression model rats were established by chronic unpredictable stress and separation.the model rats randomly divided into model group,ningxinjieyu high-dose group(10.8 mg/(kg · d)),mid-dose group(3.6 mg/(kg · d)),low-dose group(1.2 mg/(kg · d)) and Fluoxetine group(3.6 mg/(kg · d)) and the normal rats as the control group.Finally the changes of Noradrenaline (NE),Dopamine (DA),5-hydroxy tryptamine (5-HT),3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) would be observed using HPLC-ECD technique.Results Compared with the control group,the contents of NE,DA and 5-HT were significantly lower in the model group (P＜0.01).Compared with the model group,the contents of DA were increased in the different concentrations of ningxinjieyue capsule groups,and the contents of 5-HT were higher in the low-dose and high-dose groups ((272.15± 129.89) ng/g,(445.08± 127.56) ng/g,(375.33±52.38) ng/g) ; the contents of NE were increased in the low-dose and mid-dose groups ((408.08 ± 89.55) ng/g,(530.12± 149.87) ng/g,(542.53 ± 96.10) ng/g) ; the contents of HVA were increased in the mid-dose and high-dose groups; the contents of DOPAC were increased in the low-dose group; the contents of 5-HT,DA and NE were increased in the fluoxetine group(P＜0.01 or P＜0.05).Conclusion Ningxinjieyu capsule has antidepressant effect,the mechanisms might be regulated to the contents of monoamine neurotransmitters and their metabolites in cerebral cortex.

Objective To detect fetal RhCcEe genotype from fetal DNA in maternal plasma for noninvasive prenatal diagnosis.Methods DNA from maternal plasma sample was extracted by use of QIAamp DNA Kit. The existence of fetal DNA was confirmed by amplified fetal SRY gene. The fetal RhCcEe gene was amplified by polymerase chain reaction (PCR) from 30 pregnant maternal plasma. The results of fetal RhCcEe genotype were evaluated retrospectively by the serologic analysis of infant and pregnant woman RhCcEe phenotype.Results Among the 30 samples, 13 were the same phenotypes between mother and infant, 17 were different. When mother phenotypes were RhCC, cc, EE and ee homozygous, the deleted allele gene can be successfully amplified from mother plasma.Conclusion Noninvasive fetal RhCcEe genotyping is reliable. When the mother was homogyzous, genotyping the fetal CcEe alleles was very significant and useful for HDN (hemolysis disease of newborn) diagnosis and therapy.

Objective To analyze the influence of diabetes mellitus (DM) on the outcomes of percutaneous transluminal angioplasty (PTA) with stenting of femoropopliteal artery,in patients with atherosclerotic occlusive disease (ASO).Method Clinical data of inpatients who successfully received PTA with stenting procedures of femoropopliteal artery in Xuanwu Hospital from January 2006 to December 2012 were analyzed retrospectively.Patients were stratified into DM and non-DM groups.Results were compared between the two groups including primary patency (PP),assisted patency (AP),limb salvage and survival using Kaplan-Meier life table and Cox regression analyses.Result Totally 291 patients underwent 332 procedures.There were 214 DM and 118 non-DM limbs.Mean follow-up was 34 months.The 5-year PP was 23.2％,AP was 35.3％,limb salvage was 89.2％,and survival was 69.6％.DM was associated with lower 5-year PP,limb salvage,and survival than non-DM.But there were no significant difference in AP between the two groups.Females were associated with decreased PP than males on Cox multivariate analysis.Hypertension and below tibial diseases were associated with lower limb salvage rate and age is the only predictor of survival rate.Conclusions DM is a risk factor for poor outcomes after PTA with stenting procedures.

Objective To establish a PCR sequencing-based typing (PCR-SBT) method for simultaneous genotyping of human platelet antigen HPA-1 to HPA-16w.Methods All DNA polymorphism sites of HPA-1 to HPA-16w were obtained from the immuno polymorphism database.The specific primers were designed using Primer Premier 5.0 software to amplify nucleotide acid fragments encompassing each HPA polymorphism site.The primer sequence and PCR condition were optimized to obtain specific and single amplification product.The PCR product was purified and then sequenced to determine the HPA genotypes.Two standard DNA samples were detected using the HPA PCR-SBT method to examine the accuracy d this method.Sixteen reference samples (including 6 interference samples with HPA gene mutations) provided by 14th platelet immunology workshop of international society of blood transfusion (ISBT) in 2008 were also tested by this home-brew HPA PCR-SBT method.Results Total eleven pairs of primers were designed to amplify and sequence the sixteen HPA systems.The HPA genotypes of two standard samples were 1aa/2aa/3ab/4aa/5ab/6aa/7aa/8aa/9aa/10aa/11aa/12aa/13aa/14aa/15aa/16aa and 1aa/ 2aa/3aa/4aa/5aa/6aa/7aa/8aa/9aa/10aa/11aa/12aa/13aa/14aa/15aa/16aa,respectively.The 256 HPA genotypes of 16 reference samples were clear.128 genotypes among them were completely accordance with the results provided by ISBT report.Conclusions The PCR-SBT assay combining high-throughput DNA sequencer established in the study provides a simple,rapid and accurate method for HPA-1 to HPA-16w systems genotyping.The assay is suitable for routine clinical HPA genotyping and shows a broad prospect in further applications.

0.05),but the numbers of CD41+ cells and platelets were increased significantly(P

Objective To explore the evolution of research themes and trends in China's nursing college education during the last decade.Methods A co-word analysis of keywords was performed in the research literatures around nursing college education from two Chinese databases,VIP China Science and Technology Journal Database and CKNI Periodical Full-text Database,between 2003 and 2012.The analysis was based on two different periods (phase Ⅰ from 2003 to 2007 and phase Ⅱ from 2008 to 2012).Results A total of 646 articles were included,with 287 articles published in phase Ⅰ and 359 articles in phase Ⅱ.Sixteen high-frequency keywords were identified during the phase Ⅰ,with seventeen during the phase Ⅱ.Two research themes,nursing student-training model and construction and reform of curriculum,were presented in both phases.However,some differences in research sub-themes exited between phase Ⅰ and phase Ⅱ.Conclusions Hot research topics in nursing college education seemed no change in the past ten years.School-hospital collaboration newly emerged as a hot topic in the field of nurse education research.The research on construction and reform of curriculum change its focus to nursing courses and humanities courses.The academic studies of nursing college education were mostly influenced and pushed by reforming and developing of nursing education.

To study the mechanism of Shenkangling (SKL), a compound traditional Chinese herbal medicine, combined with prednisone in treating adriamycin-induced nephropathy in rats.