Objective To study the effect of listening to music with headphone or background music on pain reduction of patients undergoing colonoscopy. Methods 180 patients undergoing colonoscopy were randondy selected as the headphone group,background music group and the control group.The headphone group listened to relaxed music with headphone after measurement of vital signs.The background music group listened to relaxed music played by CD after measurement of vital signs.The control group did not listen to music.Pain degree was measured by pain intensity number scale during colonoscopy.Heart rate,blood pressure,saturation of blood oxygen and appraisal to the music by the former two groups were observed.Results Pain degree of the headphone group and the background music group was lower than that of the control group(P＜0.05).Pain degree of the headphone group was lower than that of the background music group with no significant difference(P＞0.05).Heart rate of the control group after colonoscopy was faster than before colonoscopy(P＜0.05).Conclusions Relaxed music can alleviate pain of patients undergoing colonoscopy.Listening to background music is a convenient and economical method for alleviation of pain without any side effects for patients undergoing colonoscopy.

Objective:To explore the mediating effect of self-nursing ability between living condition and anxiety in the patients with peptic ulcer caused by helicobacter pylori.Methods:By using questionnaire survey method,300 patients with a peptic ulcer caused by helicobacter pylori were assessed with the exercise of self-care agency (ESCA),Self-rated Health Measurement Scale (SRHMS) and Self-Rating Anxiety Scale (SAS).The result was compared with that of 300 healthy crowd whose general condition was well-matched.The data were processed and analyzed by using SPSS13.0 and AMOS7.0.Results:The total scores of the ESCA,SRHMS and SAS were (101 ± 13),(299 ±35),(56 ±8) respectively.The result of the correlation analysis showed there were varying degree correlation among the disease self-nursing ability,self-rated Health,and anxiety (Ps ＜ 0.01).Bootstrap test showed the size of the mediating effect of self-test health to anxiety was 0.17,the 95％ confidence intervals was 0.11-0.26,and the ratio of the mediating effect to the total effect was 0.44.Contusion:It suggests that self-nursing ability may have much strong mediating role between health condition and anxiety.

OBJECTIVE:To analyze the general features of adverse drug reactions(ADRs)caused by hepatitis B vaccine.METHODS:72national case reports of ADRs induced by hepatitis B vaccine in recent10years were classified and analyzed statistically.RESULTS:The affected patients ranged in age from1year to 40 years,with more males than females;Most ADRs occurred with in 30 minutes after the medication;The ADRs were characterized by allergies in clinic,even allergic shock.CONCLUSIONS:It is important to manage the ADRs caused by hepatitis B vaccine for the safety of medication.

OBJECTIVE:To study the compatible stability of puerarin injection with 10 commonly-used drugs.METHODS:The changes in appearance,pH,particulate and puerarin content after mixing with 10 drugs were observed and assayed.RESU_LTS & CONCLUSION:Puerarin injection mixing with adenosine triphosphate,coenzyme A and ribavirin showed significant change in pH,which indicats that they are incompatable each other,however,when puerarin injection was mixed with vitamin B6,potassium chloride,etamsylate,cimetidine,gentamycin,amikacin and erythromycin,no significant changes in pH,particulate and puerarin content were found,which indicates their compatibility.

Objective To investigate clinical and pathological features of lupus erythematosus profundus (LEP)of the scalp in children.Methods A retrospective study was carried out on 5 children with LEP.The clinical and histopathological features,treatment and prognosis of LEP were analyzed.Results The 5 children with LEP included 2 boys and 3 girls with a median age at onset of 5 months (range,2-38 months) and a median clinical course of 15 months (range,4-72 months).Clinically,the patients presented with arc-shaped or circular purple atrophic plaques on the scalp complicated by alopecia.The occipitalia and tempora were the most commonly involved sites.Antinuclear antibodies (ANA) and extractable nuclear antigens (ENAs) were negative in all the patients.Main histopathological changes were hyaline degeneration of the fat,mucin deposition and local aggregation of lymphocytes in fat lobules.Of the 5 patients,2 were treated with oral prednisone (1.5-2 mg/kg/day),1 with oral hydroxychloroquine (5 mg/kg/day),1 with oral prednisone (1.5 mg/kg/day) combined with hydroxychloroquine (5 mg/kg/day),and another 1 with topical halometasone cream and 0.03％ tacrolimus ointment.Lesions were remissed after 2-3 months of treatment,and subsided with growth of new hairs after 6 months.No recurrence was observed during a 1.5-year follow-up.Conclusion Prednisone and hydroxychloroquine are markedly effective for LEP,and pediatric patients with LEP may be treated by topical highpotency glucocorticoids and calcineurin inhibitors.

Objective To study the performance verification of lactate dehydrogenase(LDH) in the Johnson Vitros 5 .1 FS bio‐chemical analyzer .Methods According to CLSI instrumentation evaluation standard and referring to the validation scheme provided by the Johnson company ,the precision ,accuracy ,linear range of LDH ,maximum dilution degree ,biological reference range were verified .Results The LDH intra‐batch and inter‐batch precision experiments were≤3 .30% ;the accuracy experiment≤4 .00% ;the determination coefficient of the linear experiment was 0 .997 2 ;the LDH maximum dilution degree was 8 times with saline solution dilution;the biological reference range experiment verified that the reference range 313-618 U/L provided by the VITROS Meth‐odology Manual could be quote .Conclusion The performance verification of LDH detected by the Johnson Vitros 5 .1 FS biochemi‐cal analyzer basically conforms to the requirements of the quality objectives and manufacturer′s instructions ,and meets the needs of clinical test .

OBJECTIVE:To evaluate the bioavailability of two nimesulide preparations.METHODS:A total of 20 healthy male volunteers were enrolled in a randomized crossover study in which the subjects were randomly assigned to receive single dose of 200 mg nimesulide orally disintegrating tablets(test)or nimesulide tablets(reference).The plasma concentrations of nimesulide were determined by RP-HPLC,and the pharmacokinetic parameters and bioavailability were calculated with DAS program.RESULTS:The main pharmacokinetic parameters of nimesulide test and reference preparations were as follow:AUC0～24:(54.67?18.25)vs.(56.15?15.54)?g?h?mL-1;AUC0～∞:(56.38?18.03)vs.(57.63?15.26)?g?h?mL-1;Cmax:(7.61?2.72)vs.(7.50?2.19)?g?mL-1;tmax:(3.83?1.39)and(3.80?1.28)h.The relative bioavailability of nimesulide or-ally disintegrating tablets as against nimesulide tablet was(98.7?22.9)%.CONCLUSION:Nimesulide test and reference preparations were bioequivalent.

AIM: To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS: After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly ( P

Objective To investigate the expression of nestin protein and insulin-like growth factor 1(IGF-1) mRNA in rat brain injured by ischemia-reperfusion(I/R),and observe the effects of Tongxinluo,one of Chinese herb drugs,on the expression.Methods The rat model of cerebral I/R injury,reproduced by middle cerebral artery occlusion(MCAO) and release,were divided into three groups: high-dose Tongxinluo group(given 1g/kg?d of Tongxinluo],low-dose Tongxinluo group(given 0.5g/kg?d of Tongxinluo) and control group(administration of saline).2ml of Tongxinluo or saline each time was administered to each rat and 2 times every day up to day 3,5,7,14,21 and 30 after the injury.The expression of nestin in the subventricular zone(SVZ) and hippocampal dentate gyrus zone(DG) was detected by immunohistochemistry,and the IGF-1 mRNA expression around ischemic area was detected by RT-PCR.Results Compared with the control group,the immunofluorescence magnitude of nestin-positive neurons in SVZ and DG was significantly higher in high-and low-dose Tongxinluo groups(P0.05).Conclusions Tongxinluo,a kind of Chinese herb drugs,may promote the proliferation of neural stem cells in SVZ and DG after cerebral I/R injury in rats,and the mechanism may be related to upregulation of IGF-1 mRNA induced by Tongxinluo.

Abstract On May 30 2022, Changsha Center for Disease Control and Prevention received a report of multiple cases of fever from a kindergarten. A field epidemiology investigation, laboratory testing, and control and prevention work were immediately conducted. A total of 76 cases were reported, and all were students, with an attack rate of 9.93%. The onset of illness was peaked between May 18 and June 8. The main clinical symptoms were fever, cough and sore throat, and all the cases were mild. There were 39 cases tested positive for human adenovirus (HAdV) nucleic acid or antigen, and 7 nucleic acid positive specimens were identified as HAdV-7 through virus isolation, culture and genotyping. The field epidemiology investigation and laboratory testing results concluded that it was a clustered outbreak caused by HAdV-7 infection. The main reason for the spread of the outbreak might be that the kindergarten failed to report it in time. High student density, poor classroom ventilation, lax morning and afternoon inspections, non-standard daily disinfection, and the prevalence of HAdV outbreaks in the community were also associated. Kindergartens should improve its infectious disease prevention and control mechanism, strictly follow the reporting regulations, implement the three early measures, standardize disinfection work, and enhance daily ventilation to avoid similar outbreaks in the future.