Objective To investigate the antineoplastic action of 5-aza-2-deoxycytidine(CdR) in combination with arsenic trioxide on HL 60 myeloid leukemia cells.Methods HL 60 leukemia cells were incubated with CdR and arsenic trioxide and their antineoplastic action was determined by cells grouth curve,surface differentiation antigen spikes of subdiploid of cell cycle and superoxide dismutase etc.Results CdR combined with arsenic trioxide produced a greater growth inhibition andmove strong induced the differentiation of HL 60 cell than that in anyone of the two agents used independently.Conclution These results suggest that the arsenic trioxide may enhance the anti-leukemic action of CdR.

Objective To study the difgerence between the IAEA code of practice TRS-Z77 and TRS-398 in the determination of the absorbed dose to water for high-energy photon beams using several cylindrical chambers. Methods For 6 different types of cylindrical chambers,the calibration factors ND,W,Q0 in terms of absorbed dose to water were calculated from the air exposure calibration factors N,,and were compared with the ND,W,Q0 measured in European standard laboratory. Accurate measurements were performed in Varian 6 MV photon beam using 6 cylindrical chambers according to TRS-277 and TRS-398.The beam quality correction factors kQ.Q0 as well as the water absorbed doses were compared.Results For the set of chambers,the difference between ND,W,Q0 computed from Nx and ND.-.Q0 obtained in European standard laboratory was 0.13％ ～ 1.30％.The difference of beam quality correction factors for TRS-277 and TRS-398 was 0.09％ ～0.45％.The distinction of the water absorbed doses obtained according to the two different protocols was 0.27％ ～ 1.40％,and was primarily due to their different calibration factors.Conclusions The discrepancy in absorbed doses determined according to two protocols using different cylindrical chambers is clinically acceptable.However,TRS-398 allows a more convenient localization of chambers,provides a more simple formulation,and offers the reduced uncertainty in the dosimetry of radiotherapy beams.

Objective To observe the effect of interventional occlusion for the treatment of residual leak after repaired of patent ductus arteriosus (PDA).Methods A total of 23 patients with residual leak after repaired of PDA underwent interventional occlusive procedures.Original operative methods:15 patients performed surgical ligation of PDA,4 patients performed neoplasty under cardiopulmonary bypass,4 patients performed transcatheter occlusion.Twenty-three patients were treated by standard interventional occlusion.The lateral descending aortographies and echocardiography were performed to evaluate the immediate results after occlusion 15 min.Echocardiography was rechecked after occlusion 1 week,1 month to find whether there was residual shunt and recanalization.Results Twenty-three patients per formed aortographies in operation showed residual leak.The mean minimum diameter of the residual leak was 2-8 (3.7 ± 1.4) mm.After occlusion 15 min,heart murmur disappeared,the form of interventional occlusive procedures was better.One patient had little residual leak,22 patients (95.65％,22/23) residual leak disappeared.Aftertreated 1 week,1 month,aortographies and echocardiography showed the form of interventional occlusive procedures was better,all patients had no residual leak.Conclusion Residual leak after repaired of PDA can be treated safely and effectively with interventional occlusive procedures.

ObjectiveTo discuss the effect of surgical procedure and interventional procedure on coronary arterial fistula.Methods The clinical data of 25 cases of coronary arterial fistula were retrospectively analyzed.Seven cases received surgical procedures,18 cases received interventional procedures.ResultsOf the 7 cases with surgical procedures,coronary arterial fistula shunt disappeared in 6 cases after review of echocardiography,coronary arterial aneurysms was found in 1 case who had limited residual flow.Of the 18 cases with interventional procedures,12 cases were completely cured,2 cases had limited residual flow,4 cases did not succeeded.ConclusionsSurgical procedures and interventional procedures on coronary arterial fistula are safe and efficient.The choice of method should be based on the specific situation.

[Objective]To evaluate the feasibility of the minic microgravity as a method and the layered cylindric collagen-chitosan-?-tricalcium phosphate composite as a scaffold for the cartilage tissue engineering after an observation of how it absorbs the chondrocytes and affects the cell behavior.[Method]The chondrocytes were isolated and multiplied in vitro,and then the chondrocytes were seeded onto the porous collagen-chitosan-?-tricalcium phosphate composite scaffold and were cultured in both minic microgravity and ordinary environment for 3 weeks.The effects of the composite scaffold on the cell adhesivity,proliferation,morphological changes and synthesis of the extracellularmatrix were observed by the growth curve,phase-contrast microscopy,histology,scanning electron microscopy and immunohistochemistry.[Result]The chondrocytes that adhered to the scaffold increased significantly and secreted extracellular matrix in the center of the porous scaffold around the chondrocytes under minic microgravity compared with ordinary environment.Immunohistochemistry of type Ⅱ collagen was positive.[Conclusion]The minic microgravity environment will be a good method for the cartilage tissue engineering.And the layered cylindric collagen-chitosan-?-tricalcium phosphate composite scaffold has a good cellular compatibility.It will be an ideal scaffold for the cartilage tissue engineering.

Intake fraction is a new concept applied in assessing human health effect of pollutants. In the present paper, the history and practical use of it were reviewed, and then the calculation of it was also analyzed. After illustrating and discussing previous approaches for estimating intake fraction, the calculation can be classified into two sorts: one is taking the spatial area as the object, and the other is taking the people as the object. The latter is more fitful to estimate the intake fraction of mobile emission, in which it is necessary to investigate the related population and then combine the time-activity patterns with the microenvironment concentrations, in addition, modeling microenvironmental concentrations is critical for the calculation of intake fraction.

Abstract Objective:To obtain recombinant vaccinia viruses expressed HIV-lcn subtype B gag and gag-hIL-2 chimera gene,evaluate theimmune effects in experimental mice by immunization with HIV-1 DNA vaccine,observe the immune adjuvant effect of IL-2.Metbods: HIV0-1gag and gag-hIL-2 chimera gene were inserted downstream of the combined promotor of pJ38 vector respectively and recombinant vaccinia virus-es were selected by plaque essay. Expression products were examined by SDS-PAGE and Western blot.Immune response of immunized Balb/cmice was evaluated by lymphocyte transformation test,CTL quantity change of T lymphocyte of CD4+、CD8+ and the level of serum antibody.Re-sults:Recombinant vaccinia viruses vJ38gag and vJ38gag-IL-2 were obtained and the expression were confirmed by SDS-PAGE and Western-blot. Recombinant vaccinia viruses show good immune effect when immuned with HIV-1 DNA vaccine.The best strategy for immunization is therVV-primed and HIV-1 DNA vaccine-boosted group and threer times of immunization were suggested to get effective results compared withvJ38gag an incressed immune response was induced by vJ38gag-IL-2. Conclusion: HIV-1 Gag protein and IL-2 protein can be expressed invJ38gag and vJ38gag-IL-2 respectively. IL-2 show good effect to ha sued as immune adjuvant.

Objective:To explore the application of three-level storage pattern(on-line,near-line,off-line)to picture archiving and communication system(PACS),and to find a solution to the long-term storage of medical imaging data in PACS of the basic unites.Methods:To realize the on-line storage of medical imaging data by the redundant array of independent disk(RAID)of host-server;To realize the near-line storage of medical imaging data by the network attached storage(NAS);To realize the off-line storage of medical imaging data by the tape-drive.Results:The long-term storage of medical imaging data in PACS has been available in our hospital for one year since the three-level storage pattern used in PACS.It not only realizes the on-line access of enormous amount of medical imaging data,but also protects the imaging data's integrity when the on-line data damaged by human's activities or fire,etc.Conclusion:The application of three-level storage pattern in PACS,which is characterized as low expense,dependable performance,simple maintenance,can become perfect pattern in the basic Level hospital.

To investigate the degradation features of HA and PDLLA/HA composites in different proportion in vitro. Four kinds of foams consisting of PDLLA, PDLLA/HA (5%wt), PDLLA/HA (20%wt), and PDLLA/HA (35%wt) were immersed in distilled water. Changes in pH, mass loss, viscosity-average molecular weight, and strength against pressure were observed and compared to study degradation features of specimens in vitro. There were statistically differences in the indexes between the PDLLA/HA (20%wt) composite and the other foams after 12 weeks (P

Objective To study corrective method for displacement in the procedure of electronic portal imaging device (EPID)-based intensity-modulated radiotherapy dose valuation by studying the relative mechanical displacement of different vendor EPID (aS1000,Varian; aS500,Varian; iViewGT,Elekta).Methods A 5 cm × 5 cm field was set up to acquire portal images for three kinds of EPID,then a in house software was used to analysis the portal images.The relative displacement was acquired via analyzing a series of comparation between center positions of gantry angle ranging from 0° to 360° and gantry angle at 0°.Results In the lateral direction,the maximum relative displacement of EPID with aS1000,S500 and iViewGT were (-0.23 ±0.17) mm,(2.94±0.17) mm and (0.35 ±0.09) mm,respectively.In the longitude direction,the displacements were (-4.16 ± 0.20) mm,(-4.15 ± 0.25) mm and (-1.66 ±0.11) mm,respectively.As to longitude direction,the displacements could be well fitted with the usage of quadruplicate empiric function.Conclusions There is a significant difference at the aspect of relative displacement between different vendors EPID at different gantry angles.And the displacement in the longitude direction is obviously larger than in the lateral direction.The relative displacement should be corrected when applying EPID to the intensity-modulated radiotherapy dose verification at different gantry angles.