Objective To observe the characteristics of hemorheologic changes of hyperlipidemia rats at an early stage and to explore the mechanism of myocardial ischemic injury.Methods Seventy-two SD rats which were fed with high cholesterol diet(cholesterol+lard+pig bile salt) to result in hyperlipidemia model fell into HC group.Thirty-six SD rats which were fed with common diet fell into control group.The rats were fed for 10 to 90 d.At an interval of 10 d,9 time points were set.Therefore the 2 groups were randomly divided into 9 subgroups.At each time point,the changes of plasma lipids,cTnI,hemorheologic parameters,and pathological change of the rats’ myocardium and coronary vessel were assayed.Results The main characteristic of our models was hypercholesterolemia and high low-density-lipoproteins(LDL).With the extension of feeding time,plasma viscosity of HC group increased significantly,and erythrocyte deformability decreased.After fed with cholesterol for 50 d,myocardial ischemic injury occurred in the HC group.Correlation analysis revealed that the incidence of myocardial ischemic injury in hyperlipidemia rats was closely related with hypercholesterolemia and high low-density-lipoproteins.Conclusion The abnormal change of hemorheologic characteristics of hypercholesterolemia is a cause of myocardial ischemic injury.

Nine known compounds were isolated from the chlorofrom soluble fraction of ethanolic extract of Belamcanda chinensis(L. )DC.. Their structures were identified as irigenin(Ⅰ),dichotomitin(Ⅱ),irisflorentin (Ⅱ),3-stigmastenol (Ⅳ), apocynin (Ⅴ), tectoridenin (Ⅵ), ?-sitosterol (Ⅶ), 3', 4', 5, 7-tetrahydroxy-8-methoxyisoflavone (Ⅶ)and polyoctapenten (Ⅹ) by spectorscopic methods (UV, IR, EIMS, 1HNMR, 13CNMR). Compounds Ⅱ, Ⅳ, Ⅹ and Ⅻ were obtained for the first time from the plant.

To investigate the changes in the expression of intercellular adhesion molecule 1(ICAM 1) ,tumor necrosis factor ?(TNF ?) mRNA and MPO activity in rat liver after burns and the effects of escharectomy during burn shock stage on the expression of these parameters. One hundred and seventy six Wistar rats with 30% TBSA full thickness thermal injury were used. The mRNA expression of ICAM 1 and TNF ? were detected by reverse transcription polymerase chain reaction(RT PCR) in liver tissues at various intervals after burns with or without escharectomy during shock stage. Both expression of ICAM 1 and TNF ?mRNA in rat liver after burns began to increase at 4h postburn and peaked at 12h and 24h, respectively. Afterwards in groups of escharectomy at 8h and 24h postburn, the expressions of both ICAM 1 and TNF ? increased again, though the expression values were lower than the first peak values, and they returned to normal range at 96h postburn. But in groups of burn control and escharectomy at 96h postburn, their expressions still remained high on 7d postburn. In addition, MPO activity returned to normal levels at 96h postburn in groups with escharectomy during shock stage, while in groups burn control and escharectomy at 96h postburn, a high value of MPO activity persisted till 7d postburn. Our findings suggest that eschar could induce the production of inflammatory mediators such as ICAM 1 and TNF ? which damaged endothelial cells. Therefore, escharectomy as early as possible may play an important role in preventing the expression and release of adhesion molecules and the development of systemsc lnflammatory response syndrome.

OBJECTIVE： Evaluating clinical efficacy of ceftriaxone in the treatment of lower respiratory tract infections METHODS： Applying meta－ analysis to evaluation of clinical efficacy of ceftriaxone， cefotaxime and cefuroxime in the treatment of lower respiratory tract infections RESULTS ＆ CONCLUSION： Ceftriaxone is worthy of popularization and application in clinical practice because of its better efficacy and lower cost

Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. In ad-dition to genetic alterations, epigenetic inactivation of (tumor suppressor) genes has been recognized as an important and alternative mechanism in tumorigenesis. In recent years, increasing evidence has shown that ep-igenetic silencing of those genes is essential to carcinogenesis and metastasis. Epigenetics include two main forms: histone acetylation and DNA methylation, histone acetylation and DNA hypomethylation can promote gene expression,otherwise, they will inhibit gene expression. It is important to the methylation especially the hypermethylation of the tumor suppressor gene during the hepatocarcinogencsis. The study of epigenetics will be proved valuable for early diagnosis and disease monitoring at hepatocell.

OBJECTIVE:Evaluating clinical efficacy of ceftriaxone in the treatment of lower respiratory tract infections METHODS:Applying meta-analysis to evaluation of clinical efficacy of ceftriaxone,cefotaxime and cefuroxime in the treatment of lower respiratory tract infections RESULTS & CONCLUSION:Ceftriaxone is worthy of popularization and application in clinical practice because of its better efficacy and lower cost

Objective To investigate the methods how to improve condition of procreative health of childbearing disabled women for planned parenthood and good procreation and good education.Methods The method of whole flock sampling of many stages and many layers were taken and the questionnaire and group interview were performed to collect the data of quantity and analyze the affect factors of disabled women about their marriage,procreation,procreative health,and knowledge about procreative health.Results There were 4 380 cases of validity questionnaire.62.33% of them married.In 3 005 procreated women,45.49% had one baby,45.49% had two babies and 8.95% had three babies or more.46.54% of them denied that they had infection of reproductive system.63.19% of them had been examined in department of gynecology.63.17% of them took birth control measure.29.70% of them were lost in taking birth control measure.50.24% of them could say three methods of birth control.37.39% of them could say three ways about AIDS infection.Conclusion Childbearing disabled women have some troubles in birth control,procreative health,and their knowledge about procreative health.

BACKGROUND:Nano-hydroxyapatite/bacterial celulose (nHA/BC) composite scaffolds prepared by nano-bionics method exhibit a structure and performance similar to natural bone. OBJECTIVE:To study the cytotoxicity and biocompatibility of nHA/BC composite scaffolds. METHODS:(1) Acute systemic toxicity test: nHA/BC leach liquor and normal saline were respectively injected intraperitonealy into Kunming mice. After 24, 48, 72 hours, the body mass of mice was recorded. (2) Sensitization test: nHA/BC leach liquor and normal saline were respectively injected subcutaneously into the back of Japanese white rabbits. With 72 hours of injection, edema and erythema were observed, and the experiment was done again at an interval of 14 days. (3) Pyrogen test: nHA/BC leach liquor and normal saline were respectively injected into the ear vein of Japanese white rabbits, and after injection, the body temperature of rabbits were determined. (4) Hemolysis test: Diluted rabbit anti-coagulant was added into nHA/BC leach liquor, normal saline and distiled water, respectively. (5) Passage 3 bone marrow mesenchymal stem cels from rabbits were co-cultured with nHA/BC materials, and then, cel proliferation, growth and adhesion on the material surface were observed. RESULTS AND CONCLUSION: nHA/BC composite scaffold was of no acute systemic toxicity, non-alergenic, no pyrogenic and hemolytic reactions; nHA/BC had a three-dimensional porous structure that was better for growth, proliferation and adhesion of rabbit bone marrow mesenchymal stem cels. These findings indicate that the nHA/BC composite scaffold has good biocompatibility and cytocompatibility.

AIM:To establish the quality standard for Pheretima Dispensing Granules.METHODS:Pheretima Dispensing Granules were identified by TLC.The content of hypoxanthine in Pheretima Dispensing Granules was determined by HPLC,and succinic acid by high performance capillary electrophoresis HPCE.RESULTS:Spots obtained from the test solutions had the same color as amino acid reference solution,Pheretima reference substance and the blank solution had no interference.The contents of hypoxanthine were in the range of 1.6 mg/bag-3.3 mg/bag and succinic acid 23.58 mg/bag-35.38 mg/bag in Pheretima Dispensing Granules,respectively.CONCLUSION:The methods of identification and quantification were accurate,realizable and reproducible.It can be used effectively for the quality control for Dispensing Granules.

Objective:To make an ideal model for liver transplantation. Methods:On the basis of cuff technique,we carried out 62 cases of orthotopic liver transplantation in 3 months. In the transplantation,we anastomosed the portal vein and subhepatic vena cava with cuff and conneted the suprahepatic vena cava to the recipient's diaphragm with 9-0 running suture. Results:The average time of acquiring donor liver was 32 minutes, reqairing liver was 12 minutes,unhepatic phase was 18 minutes.62 liver transplantation operations were carried out ,in which the operation success rate in late 30 cases was 90%. Conclusion:The results show that the model is stable and can be used in the experiment of liver transplantation.