Objective To understand the status quo of low vision and the potential influential factors among primary and second-ary school students in Kunming .Methods The visual activity test and the questionnaire survey were performed among 1 366 prima-ry and secondary school students in a community of Kunming ,χ2 and the multivariate Logistic regression were adopted to perform the single factor and multifactor analysis .Results The detection rate of low vision in this community was 57 .5% ,which of the sec-ondary school students was higher than that of primary school students (70 .7% vs .46 .4% ,χ2 =81 .966 ,P=0 .000) .The multifac-tor Logistic regression analysis showed the bad habit of using eyes (OR=3 .176) ,poor posture(OR=2 .844) ,continuous learning time ≥2 h per day(OR=1 .743) ,break bad behavior(OR=1 .952) ,the screen contact time ≥2 h per day(OR=1 .660) and continu-ous doing homework time ≥ 2 h per day(OR=1 .535) were the risk factors leading to the low vision ;while the outdoor activities time ≥ 2 h per day(OR=0 .059) were the protective factors .Conclusion The detection rate of low vision among primary and mid-dle school students in Kunming is higher .Cultivating the good habits using eyes ,alleviating the study load ,reducing the screen con-tact time ,and ensuring the time of outdoor activities and sleep time will conduce to protect the eyesight of primary and secondary school students .

A new method based on the multiple locus variable number tandem repeat analysis (MLVA) was applied for the genotyping of combined HIV Mycobacterium tuberculosis in Dali to investigate the genotyping and distribution pattern of Myco‐bacterium tuberculosis clinical isolates with MLVA .Mycobacterium tuberculosis clinical isolates were selected from Dali area , and the polymorphism of VNTR locus was tested with PCR .The clustering of genotype was analyzed by BioNumerics (6 .6) . Result showed that 15 VNTR loci of 61 combined HIV Mycobacterium tuberculosis clinical isolates were analyzed respectively . There were obvious polymorphisms of VNTRs .The discrimination power of these loci appeared different from each other ,with the biggest Hunter‐Gaston index (0 .839) loci was MIRU26 ,and the smallest one (0 .341) loci was MIRU4 .The clustering of genotype showed that these strains could be categorized into 5 gene clusters and 61 genotype ,the proportions of cluster Ⅰ was the biggest one ,51 .6% were cluster Ⅰ which including 32 strains .The standard strain H37Rv was belongs to cluster Ⅱ .Its indicated that there are obvious polymorphisms of VNTRs of combined HIV Mycobacterium tuberculosis clinical isolates in Da‐li .The main genotype was Beijing family genotype .

Objective To evaluate the short term and long term outcomes of primary percutaneous transluminal coronary angioplasty (PTCA) for acute anterior wall myocardial infarction (AMI) complicated with pump failure Methods 28 patients admitted within 12 hours after the onset of AMI and complicated with heart failure underwent primary PTCA and stent implantation The short term and long term therapeutic effects were observed Results The infarct related coronary arteries (IRCA) of the 28 patients were all LADs with occlusions at the proximal or beginning segment and TIMI flow was 0 Ⅰ Single vessel disease was showed in 15 patients,double vessel disease in 9 and triple vessel disease in 4 The immediate procedural success rate was 100% with TIMI flow Ⅲ in all IRCAs One patient without stent implantation had a recurrent chest pain 2 days after procedure and died of acute pulmonary edema During 1～36 month follow up of the 27 survivors, no death occured and the average LVEF was showed 59 8?6 5% by echocardiography Conclusion Primary PTCA for AMI complicated with pump failure was safe and effective to improve the short term and long term outcomes

Objective To evaluate the efficacy and safety of intravenous thrombolysis with urokinase combined with emergency interventional therapy for acute myocardial infarction(AMI). Methods Fifty two patients with first AMI (≤12 h from onset)were randomized to thrombolysis plus PCI group and primary PCI group,the patency rate of infarct related artery (IRA) before intervention,the procedural success rate,the incidence of bleeding complications and acute ischemic events during hospitalization and the left ventricular ejection fraction (LVEF) measured by echocardiography before discharge were compared.Results The IRA patency rate in the thrombolysis plus PCI group (61 5%) was significantly higher than that in the primary PCI group (19 2%) ( P 0 05),no major bleeding complication and acute ischemic event occurred during hospitalization in both groups,the LVEF in the thrombolysis plus PCI group (64 3?5 6)% was higher than that in the primary PCI group(54 8?4 9)% before discharge ( P

Objective To study the protective effect of a hyperoxic solution on phosgene-induced lung injury by observing the changes in W/D ratio, lung water (LW), and L/B, and MDA contents, GSH-PX activity, and protein contents in broncho-alveolar lavage fluid (BALF). Methods The rabbits were divided into normal control group, hyperoxic solution (HO) and balance salt(BS) groups.Group HO and Group BS inhaled phosgene, and hyperoxic solution was given intravenously in group HO, but BS was given in group BS. W/D, LW, L/B, and MDA contents,GSH-PX activity,protein contents in broncho-alveolar lavage fluid (BALF) were determined. Results The MDA contents, W/D, LW and L/B were increased, and GSH-PX activity was decreased significantly in Group HO and Group BS compared with control group (P

Objective To investigate whether oral propofol has any inhibitory effects and dose-response relationship on the pain inducing tissue injury in mice. Methods The effect of propofol on pain was observed in formalin test and acetic acid writhing test in mice. Formalin was injected subcutaneously into the plantar surface of one hind paw. Spontaneous nocuous responses were immediately scored by counting the number of flinches of the injected hindpaw at every 5-minute interval during a 60-minute period. The number of writhing caused by intraperitoneal injection of 0.6% acetic acid was also observed in mice. Results Oral propofol in a dose of 100mg/kg did not significantly inhibit nocuous stimulation. With higher doses, propofol inhibited both the phases 1 and 2 of persistent spontaneous pain induced by subcutaneous formalin injection. Orally taken propofol also inhibited the number of writhing after intraperitoneal acetic acid injection in a dose dependent manner. Conclusion Oral propofol is effective in inhibiting pain induced by formalin and acetic acid.

Objective To investigate the clinical effect of atorvastatin combined with psychological intervention in elderly patients with coronary heart disease. Methods According to the different treatment and nursing intervention mode will be January 2015 to April 2017 year in April in our hospital 120 patients with coronary heart disease groups: control group with routine nursing intervention mode enalapril treatment, the observation group used atorvastatin+psychological nursing intervention therapy; the two groups of patients with clinical symptoms, improve psychological status and the incidence of adverse reactions were recorded, and the related data for comparative analysis. Results Atorvastatin statin+psychological nursing mode (observation group) intervention in the treatment of senile coronary heart disease clinical effect is better than that of enalapril+routine nursing (control group) treatment intervention, patients with clinical symptoms improved better than the control group, the mental state improved better than the control group, the incidence rate of adverse reaction was lower than the control group, the difference was statistically significant (P< 0.05). Conclusion The effect of atorvastatin + psychological nursing intervention in elderly patients with coronary heart disease is remarkable. It can effectively improve the clinical symptoms and psychological status of patients, and have less adverse reactions. It is worthy of clinical application.

Objective To observe the effect of doxofylline and budesonide combined with psychological intervention on the treatment of the patients with bronchial asthma, and evaluate its clinical effect. Methods From March 2014 to February 2017 in Guangfu hospital, 148 cases with bronchial asthma were divided into the control group and the experimental group by randomized double blind digit table with 74 cases in each group. The control group were given doxofylline and budesonide combined with routine nursing intervention, at this basis, the experimental group were receive psychological intervention. The clinical effect in the two groups were compared. Results The total effective rate was 95.95% (71/74) in the experimental group while 79.73%(59/74) in the control group, the difference was statistically significant between the two groups(P<0.05); Compared with before treatment, FEV1, FVC, VC and other indicators after treatment were significantly improved, and the experimental group improved significantly better than those in the control group (P<0.05). Conclusion Doxofylline and budesonide combined with psychological intervention on the treatment of the patients with bronchial asthma has obvious curative effect, which can effectively improve the lung function, accelerate the symptoms disappeared time, and worthy of clinical application.

Objective To investigate whether spinal cord is involved in the analgesic effect of propofol.Methods Fifteen adult SD rats of both sexes weighing 195～223g were randomly divided into three group of five animals each: control group received normal saline intraperitoneally(ip), fentanyl group received fentanyl 0 1mg/kg and propofol group propofol 100mg/kg ip 2 min later 4% formalin 150?l was injected subcutaneously into the planta region of right hindpaw 1h after formalin injection animals of all three groups were anesthetized with pentobarbital sodium ip After induction of anesthesia chest was open and 100 ml of normal saline was infused via left ventricle then followed by 4% formalin infusion for fixation of tissue, 90 min later spinal cord, L 3 5 sect, was removed for determination of c fos expression in spinal cord using fos immunohistochemistry technique Results In control group in less than 10 s after formalin intraplantar injection the animals were agitated, restless, lame and paw licking In fentanyl group and propofol group the righting reflex was suppressed for (19 4?7 8) min and (7 2?1 5)min and no pain response was seen during this period When righting reflex recovered the pain response was much lighter than that in the control group Formalin stimulation induced c fos expression was seen only in the ipsilateral spinal cord Both fentanyl and propofol significantly suppressed c fos expression evoked by formalin stimulation The number of fos like immunoreactivity neurons(FLIN) decreased by 57 8% and 36 3% respectively(P

Objeetive:To screen the different extracts from Geranium strictipes with anticaries activity,and to study the effect of the active extract on the production of acid and polysaccharides of oral cariogenic bacteria.Methods:Chlorhexidine and Galla Chinensis were used as the positive controls.Tube double dilution method was used to study the extracts of Geranium strictipes on the growth of Streptococcus mutans (S.mutans) and Actinomyce viscosus (A.viscosus).First,the activity of 5 extracts fom Geranium strictipes against S.mutans,A.viscosus was detected.Their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were obtained.The effects of 4 lower concentrations(＜ MIC) of the active extract on the production of oral bacterial acid polysaccharides were examined.Results:The effect of n-butanol extraction part of Geranium strictipes was the strongest among all the extracts against the two kinds of bacteria in the inhibition of bacteria growth,bacterial acid production and polysaccharides production,but weaker than that of Chlorhexidine and Galla Chinensis.Conclusion:N-butanol extraction part of Geranium strictipes may be a natural and effective anti-caries agent.