Objective To explore cardiovascular disease in postmenopausal women with polycystic ovary syndrome(PCOS) phenotype.Methods This study was a prospective follow-up study which included subjects with PCOS phenotype admitted from Jan.1st, 2005 to Dec.31st, 2007 in Gynecology Department of Dongyang People's Hospital.These subjects were divided into two groups according to the presence or absence of PCOS phe -notype and were followed .Kaplan-Meier method was used to estimate survival rate .The survival difference be-tween the two groups was compared using the log-rank test.Multivariate Cox proportional hazards regression was used to determine the independent risk factors of major adverse cardiac events ( MACE) .Results 11 patients in non-PCOS phenotype group had MACE , and 6 patients in PCOS phenotype group had MACE .PCOS phenotype group had a significantly decreased MACE-free survival(χ2 =4.957,P=0.026).Multivariate Cox proportional hazards regression analysis showed that low level of HDL-C, presence of PCOS phenotype and high level of hs-CRP were independent predictors for MACE .Conclusion PCOS phenotype is an independent risk factor for car-diovascular disease in postmenopausal women , help to make further risk stratification and shows cardiovascular disease is closely related to PCOS .

Objective To investigate the effects of humanistic care on primiparas' role competency during pregnancy and delivery.Methods Data of 21 cases were collected using semi-structured interviews and phenomenological qualitative method was used to analyze the data.Results The effects of humanistic care on maternal role competency during pregnancy included:to participate in related curriculum,to get ready for delivery actively.The effects of humanistic care on maternal role competency during delivery included:nurses' carefulness gave psychological support,nurses' instruction gave professional support,nurses' encouragement gave spiritual power,nurses' support enhanced belief,successful delivery experience gave.satisfaction.Conclusions Humanistic nursing is helpful to improve delivery experience,to increase maternal satisfaction and confidence,to promote matemal role competency and harmonious relationship between mothers and nurses.

To develop a method for the quality control of Astrgalus membranaceus (Fisch.) Bge. powder for injection (AMPI). Methods　TLC-spectrophotometry was used to determine the content of astragaloside A in AMPI. The mobile phase was chloroform-methanol-water (65∶30∶10) and detected at the wave length of 418 nm. Results　The regression equation within the spotted range of 80～240 μg was A=1.538 C+ 0.189, with good linearity, r=0.999 8. The average recovery rate was 98.68% and RSD=2.52%(n=5). Conclusion　The method used simple and easily available instruments and the rusults obtained were reliable and specific, suitable for the quality control of AMPI.

Object To develop a method for the quality control of Astrgalus membranaceus (Fisch ) Bge powder for injection (AMPI) Methods TLC spectrophotometry was used to determine the content of astragaloside A in AMPI The mobile phase was chloroform methanol water (65∶30∶10) and detected at the wave length of 418 nm Results The regression equation within the spotted range of 80~240 ?g was A=1 538 C+ 0 189, with good linearity, r=0 999 8 The average recovery rate was 98 68% and RSD=2 52%(n=5) Conclusion The method used simple and easily available instruments and the rusults obtained were reliable and specific, suitable for the quality control of AMPI

Among inorganic salts tested, K 2HPO 4 was more essential to the sclerotia for mation and carotenogenesis of strain PT95 than KCl, MgSO 4 or FeSO 4 It wa s also shown that the combination of K 2HPO 4, KCl and MgSO 4 could produce t he best positive cooperation and give the highest sclerotia biomass (782mg/plate ) and pigment yield (328 ?g/plate) Five carbon sources, i e glucose sucros e, lactose, maltose and soluble starch, all could be utilized by the strain PT95 , and maltose was the best Among 8 nitrogen sources, yeast extract favooured t he sclerotia formation, and peptone fovoured the pigment accumulation; amine sal ts and urea were unfavourable to form sclerotia The medium containing 0 24 ～ 0 48 g/L sodium nitrate nitrogen was effective to both the sclerotia formation and the carotenoid production of strain PT95 when available maltose carbon con centrations were at 5 26～21 05 g/L The optimal C/N ratio was found to be 25 ∶1

Objective To explore the relationship between nonalcoholic fatty liver (NFALD)and apolipoprotein E (ApoE)gene polymorphism in Shanghai undergraduate students. Methods A cross-sectional survey was performed in 4 148 students from Shanghai Tongji University. Quesgionary, physical measurement, ultrasonography and biochemical parameters were used to diagnose NFALD. In patients with NAFLD and 200 healthy subjects (served as control), ApoE genotypes were determined by PCR-RFLP. Results (1)Totally 398 subjects were diagnosed as NAFLD (prevalence 9.6%). Body mass index, waist-to-hip ratio, triglycerides, total cholesterol, alanine transaminase, aspartate aminotransferase and γ-glutamyltransferase in NAFLD group were higher than those in control, which were related to the degree of NAFLD (P＜0.01). (2)ApoE genotypes distributions were significantly different between two groups (χ2=10.52, P＜0.01). In NAFLD,E4/4 genotype frequency (5.5%)increased, and E3/3 (61.8%)decreased . (3)Unconditional logistic regression showed that E4/4 were closely associated with lipid profiles. Conclusion ApoE point mutations 112 and 158 may cause different dyslipidosis. E4/4 genotype seems to be one of the genetic predisposition factors of NAFLD.

Objective To investigate the effect of pancreatic ? cell dysfunction on abnormal glucose tolerance during Graves hyperthyroidism. Methods We investigated 6 healthy people and 36 Graves disease patients with abnormal glucose tolerance, and determined blood sugar and insulin at 0, 60 and 120 minutes after breakfast respectively. Then the HOMA-IR, HOMA-? and ISI were compared between the two groups. Results The HOMA-IR in the Graves disease patients with abnormal glucose tolerance is evidently higher than in the healthy subjects (P

Objective To investigate the effects of quercetin on the proliferation of mammary cancer cell in vitro. Methods Effects of quercetin on the cell proliferation was tested in ER-positive MCF-7 cell and ER-negative MDA-MB231 cell by MTT measurement. And to evaluate the estrogen-like effect of quercetin and its relation with the estrogen-receptor by pure estrogen receptor antagonist ICI 182, 780 as a tool. Cell cycle was examined by flow cytometry. Result Quercetin (10～50 ?mol/L) stimulated proliferation of ER-positive MCF-7 cell compared with solvent control, whereas ER-negative MDA-MB231 cell proliferation effect was inhibited, and the cell cycle was impulsed from G1 to S, DNA synthesizing was inhanced, PI was also increased. The above function on boosting MCF-7 cell proliferation can be inhibited by adding estrogen receptor antagonism ICI182, 780. Conclusion Quercetin has the estrogen-like activities through the estrogen response pathway.

Objective To study the condition for induction and differentiation of callus and propagation of adventitious buds in lamina,stem,and bud of Bupleurum chinese and establish a new method for rapid propagation.Methods In MS media added with different phytohormones,calli were induced from explants of lamina,stem,and floral bud of B.chinese,adventitious buds and adventitious roots were differentiated from calli of stem and floral bud,test-tube plantlets were formed.Results MS Medium added with 2,4-D 1.0 mg/L,KT 0.5 mg/L,and 6-BA 0.5 mg/L was suitable for calli induction of the lamina,stems,and floral buds.In medium added with 6-BA 1.0 mg/L,NAA 0.03 mg/L,CM 15% and CH 500 mg/L,the differentiation rate of floral buds callus was the highest.MS Medium added with 6-BA 1.5 mg/L,NAA 0.05 mg/L and CH 250 mg/L was suitable for propagation of test-tube plantlets,1/2 MS medium added with NAA 0.5 mg/L was suitable for rooting.Conclusion A great deal of test-tube plantlets could be differentiated and propagated rapidly by calli induced from stems and floral buds of B.chinese.Then the regeneration plantlets with normal growth and development are obtained.

Objective To research the acute adverse effects of air pollution on the risk of stroke in the air pollution exposed people.Methods Daily counts of strokes occurrence (2004-2007) were obtained from the population-based stroke registry in one district of Shanghai.Air pollution data was obtained from the Shanghai Environmental Monitoring Center.A semi-parametric generalized additive model was used to study the acute effects of air pollution on stroke occurrence after controlling for long-term and seasonal trends,weather variables,and day of the week.Results The relative risks of stroke occurrence for per 10 ?g/m3 increase of PM10,SO2 and NO2 were 1.02 (95%CI:1.01-1.03),1.05 (95%CI:1.04-1.06) and 1.09 (95%CI:1.08-1.10),respectively.Conclusion The study has provided a new evidence for the association between air pollution and risk of stroke occurrence.