Objective To study the effect of maternal hypertensive disorders complicating pregnancy on brain development of the neonates. Methods Insular area,circle and cerebral gyri width of lobus frontals were measured in 56 neonates of the mothers with hypertensive disorder complicating pregnancy(HDCP)by ultrasound and were compared with those of 53 neonates of the mothers without HDCP(control group). Results In the control group,the cerebral gyri width of term neonates was narrower than that of preterm neonates,while the insular circle was longer and the insular area was larger in the term neonates(P<0.01).Insular circle was significantly shorter and the insular area was significantly smaller in neonates of HDCP mothers [term:(87.43±10.21)mm,(413.60±90.05)mm2;preterm:(72.59±7.50)mm,(285.71±48.62)mm2]than those of the corresponding neonates in the control group[term:(102.14±9.97)mm,(557.08±98.89)mm2:preterm:(78.42±10.14)mm,(356.01±93.07)mm2].Cerebral gyri width were significantly wider only in the full term neonates.The incidence of small gestational age neonates was 30.36%(17/56)in hypertensive disorder complicating pregnancy group.The neonatal behavioral neurological score of hypertensive disorder complicating pregnancy group was significantly lower than that of control group (P<0.01). ConclusionsMaternal hypertensive disorder complicating pregnancy may result in poor development of brain of their neonates,which could be evaluated by cerebral ultrasound.

Objective:To investigate the clinical application of and critically ill patients received comprehensive nursing interventions during mechanical ventilation effect value. Methods:Ninety eight patients were selected in 2012 July~2013 year in July in our hospital, were treated with mechanical ventilation, were randomly divided into observation group and control group, 49 cases in each group, the control group using ICU routine care, the observation group in the control group on the basis of comprehensive nursing intervention, observation and nursing effect of the two groups. Results:The group of patients with mechanical ventilation time, hospitalization time was shorter than the control group, comparing the data between the groups, the difference was statistically significant(t=8.3678, t=5.9103; P<0.05). The observation group patients with unplanned estuation, ventilator-associated pneumonia was lower than the control group, the mortality rate was lower than the control group, comparing the data between the groups, the difference was statistically significant (x2=5.0178, x2=10.2424, x2=5.9905;P<0.05). Conclusion:in critically ill patients were given comprehensive nursing intervention can shorten the duration of mechanical ventilation and hospitalization in patients with mechanical ventilation, to reduce the unplanned extubation and ventilator-associated pneumonia, reduce mortality, it is worth promoting in clinical use.

Object To develop a method for the quality control of Astrgalus membranaceus (Fisch ) Bge powder for injection (AMPI) Methods TLC spectrophotometry was used to determine the content of astragaloside A in AMPI The mobile phase was chloroform methanol water (65∶30∶10) and detected at the wave length of 418 nm Results The regression equation within the spotted range of 80~240 ?g was A=1 538 C+ 0 189, with good linearity, r=0 999 8 The average recovery rate was 98 68% and RSD=2 52%(n=5) Conclusion The method used simple and easily available instruments and the rusults obtained were reliable and specific, suitable for the quality control of AMPI

To investigate and analyze the factors which influence the public attitudes towards autopsy after accidental deaths.The study has carried on an investigation among 386 individuals in random,with a questionnaire named "The cognition of the public whether to carry on the autopsy after accidental deaths".Using the statistical methods of the Logistic Regression analysis and optimum regression analysis to analyze the recycling questionnaires.Through the diagnosis,we found there are four main factors which influence public opinion upon the autopsy after accidental deaths,the knowledge of autopsy,the believing in autopsy,years of schooling and family financial circumstances.

Objective:To establish an efficient method for expression and purification of vMIP-Ⅱ which encoded by a viral gene and ho-mogued human chemokine in active single strand in E.coli cells. Methods: Cloning with bi-enzyme restriction and lysising bacteria with cold osmoic shock were used for expression,MBP affinity chromatography of fusion protein and self-selective of recombinative peptide for final purification. The expressing and purifying products were detected with SDS-PAGE and Western blotting and identified with inhibitory adhesion experiment for its activity.Results: Fusion protein MBP-vMIP was effeciendy expressed in E. coli at secretive type, and self-cleaved to sparate the vMIP-Ⅱ. The final product single strand vMIP-Ⅱ was active for blocking chemokine receptor CCR5.Conclusion:This is an effective method for obtaining viral chemokine vMIP-Ⅱ of recombinant single strand.The recombinant vMIP-Ⅱ may be useful for the study of diseases involving in chemokiine receptors such as HIV infection,rejection of transplantation and chronic inflammation,etc.

To develop a method for the quality control of Astrgalus membranaceus (Fisch.) Bge. powder for injection (AMPI). Methods　TLC-spectrophotometry was used to determine the content of astragaloside A in AMPI. The mobile phase was chloroform-methanol-water (65∶30∶10) and detected at the wave length of 418 nm. Results　The regression equation within the spotted range of 80～240 μg was A=1.538 C+ 0.189, with good linearity, r=0.999 8. The average recovery rate was 98.68% and RSD=2.52%(n=5). Conclusion　The method used simple and easily available instruments and the rusults obtained were reliable and specific, suitable for the quality control of AMPI.

OBJECTIVETo observe the clinical efficacy on constipation predominant irritable bowel syndrome (IBS-C) treated with acupuncture for regulating the mind and strengthening the spleen and the impacts on the quality of life in the patients.

METHODSSixty patients of IBS-C were randomized into two groups. In the acupuncture group (30 cases), acupuncture was applied to Tianshu (ST 25), Zusanli (ST 36), Shangjuxu (ST 37), Taichong (LR 3), Sanyinjiao (SP 6), Yintang (GV 29) and Baihui (GV 20), once a day, 5 treatments a week and 4 weeks treatment as one session. In the western medication group (30 cases), lactulose oral solution was prescribed, 15 mL each time, three times a day, 4 weeks treatment as one session. The symptom grade scale and the quality of life scale (IBS-QOL) were adopted to observe the changes in clinical symptoms and quality of life before treatment, after treatment and 2-month follow-up after treatment in the patients of the two groups separately.

RESULTS(1) Clinical symptom score: the difference in the symptom score at each time point was significant statistically in intra-group comparison (P < 0.01). In 1 week, 4 weeks of treatment and 2 months after treatment, the clinical symptom scores in the acupuncture group were lower than those in the western medication group (P < 0.05, P < 0.01). (2) IBS-QOL score after treatment was improved as compared with that before treatment in the two groups (P < 0.01). The improvement of IBS-QOL at 2 months after treatment in the acupuncture group was apparently superior to the western medication group (P < 0.05). (3) The total effective rate was 90.0% (27/30) in the acupuncture group and was 83.3% (25/30) in the western medication group. The overall efficacy in the acupuncture group was better than that in the western medication group (P < 0.01).

CONCLUSIONAcupuncture for regulating the mind and strengthening the spleen significantly relieves the clinical symptoms of IBS-C and improves QOL of patients. The total efficacy is superior to lactulose oral solution and presents a certain of long-term efficacy.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Constipation ; physiopathology ; therapy ; Female ; Humans ; Irritable Bowel Syndrome ; physiopathology ; therapy ; Male ; Middle Aged ; Quality of Life ; Spleen ; physiopathology ; Treatment Outcome ; Young Adult

Objective To study the effect of maternal diabetes during pregnancy on brain deve lopment of filial generation. Methods Cerebral gyri width of lobus frontals was measured in 79 infants of pregnant diabetic mother by ultrasound and the parameters were compared with 108 infants of non diabetic mother. We analyzed the relationship between the variation of cerebral gyri width and the diabetic control of the mothers. Brain development status in neonates with diabetic mothers was observed. Results Cerebral gyri width were significantly wider in the full term infants of diabetic mother (1.006?0.102)cm than that of the infants of non diabetic mother (0.859?0.087)cm. This was more obvious in the gyrus frontals superior and initial section[(1.167 ?0.197)cm vs (0.956 ? 0.123) cm]( P 0.05). Conclusions Maternal diabetes in pregnancy may result in filial generation brain immaturity. Cerebral ultrasound can be used to measure the cerebral gyri width.

Objective To investigate the distribution characteristics of bone marrow(BM)cell morphological results and their role in the etiological diagnosis of blood diseases,and to understand the characteristics of hematological diseases spectrum in this ar-ea.Methods The BM puncture specimens in 372 cases of hematological diseases in our hospital from March 2012 to April 2014 were performed the Wright-Giemsa staining and cytochemical staining.The cell morphology and cytochemical staining were ob-served by microscope,which was combined with the related clinical data for obtaining the morphological report.Results Among 372 cases,368 cases were diagnosed with hematological diseases,which were dominated by 3 kinds of main type,leukemia(95/372), hyperplasia anemia(36/372)and iron-deficiency anemia (26/372).In 82 cases of anemia,hyperplasia anemia(36/82),iron-deficiency anemia (26/82)and megaloblastic anemia (11/82)were predominant.Conclusion The BM morphological examination is one of im-portant measures for the etiological diagnosis in hematological diseases.The analysis on the diseases spectrum is conducive to guide the diagnosis and treatment in clinic.

BACKGROUND: Generally speaking, neuroendocrine cells have been not observed in normal breast tissue but found in breast carcinoma tissue which was affected by local microenvironment and hormone level during differentiation of breast epithelial stem cells.OBJECTIVE: By detecting expressions of breast cancer resistance protein (BCRP), cytokeratin-8 (CK8), and chromogranin-A (CgA) in breast carcinoma tissue, to explore the possible mechanism of neuroendocrine cells observed in breast carcinoma tissue during differentiation of multi-lineage potential of bone marrow mesenchymal stem cells.METHODS: BCRP, CK8, and CgA were used as markers for SP stem cells, glandular epithelium differentiation, and neuroendocrine differentiation, respectively. Immunohistochemistry was used to detect the expressions of BCRP, CK8, and CgA in breast tissues of 89 subjects and analyze their correlation. RESULTS AND CONCLUSION: Both BCRP and CK8 expressions were observed in normal breast tissues, hyperplastic tissues, and breast carcinoma tissue. BCRP expression was increased in the breast carcinoma tissue; CK8 expression was decreased with the abnormal differentiation of breast tissue; CgA expression was only detected in breast carcinoma tissue. BCRP expression was significantly correlated with positive CgA expression (P < 0.01), but it was no correlation with positive CK8 expression in normal breast tissues, hyperplastic tissues, and breast carcinoma tissue (P=0.069). The results suggested that neuroendocrine cells were not observed in both normal breast tissues and hyperplastic tissues but in breast carcinoma tissue, which possibly correlated to differentiation of multi-lineage potential of bone marrow mesenchymal stem cells.