Objective To determine the effect of prolonged hyperoxia on neonatal rat lung. Methods Full term and premature newborn SD rats were continuosly exposed to 85% oxygen or room air 7 and 14 days after birth.The activities of 3 different kinds of antioxidant enzyme (AOE) including superoxide dismutase(SOD), glutathion peroxidase (GP) and catalase (CAT) in supernatant fractions of lung homogenates were assessed after 7 and 14 days of exposure. So was the lung hydroproline content. Results (1)AOE acctivitis: Except CAT activity at 14 days of exposure, others AOE activities in O 2 exposed rat pups were significantly higher than those in air exposed controls (P

OBJECTIVE To evaluate the in vitro inhibitory effects of antibacterials in combination carbapenem against multiresistant Pseudomonas aeruginosa.METHODS The protocol was designed by checkboard method and the MIC of the two antibiotics in combination was determined by broth dilution method.FIC was calculated according to MIC results.RESULTS The MIC50 and MIC90 of eight kinds of antibiotics were lower to 1-16 times and 1-64 times,respectively.The synergistic and additive antibacterial activity was seen in ceftazidime with amikacin,piperacillin with tobramycin and azlocillin with sulbactam.The lomefloxacine with ampicillin and lomefloxacine with ceftriaxone were not correlated.CONCLUSIONS Antibacterial activity of ceftazidime combined with amikacin against carbapenem-multiresistant P.aeruginosa is the best.

Objective To study the effect of different dose of propofol compounding with epidural anesthesia on oxygenation and shunt fraction during one-lung ventilation(OVL).Methods Forty patients with esophageal carcinoma undergoing thoracotomy were randomly divided into group Ⅰ and Ⅱ.After placing epidural tube at T_(6～7) for epidural anesthesia,the patients group Ⅰ were received propofol 5mg?kg~(-1)?h~(-1) and 10mg?kg~(-1)?h~(-1) in group Ⅱ.Taking the blood sample from arter for blood-gases measurement at beginning of operation,30min after OVL and 60min after OVL.Results At 30min after OVL,the shunt fraction(Qs/Qt) value of group Ⅰ and Ⅱ was(11.35?3.6) and(15.6?5.41);PaO_2 was (36.12?10.23) and(31.05?9.36).At 60min,the Qs/Qt was(13.9?3.7) and(18.5?2.1);PaO_2 was(34.87?15.42) and(26.97?11.35).There was significantly difference between two groups(P

[Objective] To observe effects of HQSM on CK, LDH and AST activity in serum of acute myocardial ischemia rats caused by injecting pituitrin.[Method] SD rats were divided into several groups at random: normal control group,model control group, three HQSM groups(210mg/kg,150mg/kg,105mg/kg), positive control group(Huangqi Shengmaiyin and Diao Xinxuekang).The rats were treated with respective drugs by ig for 30 days, and pituitrin was injected respectively by caudal vein 1h after administering at the 29th and 30th days.Blood serum was taken out to determinate the activity of CK, LDH and AST at 1h and 24h after the last injection. [Result] The activity of LDH and AST in serum increased at 1h after injecting pituitrin, the activity of CK in serum increased,meanwhile LDH and AST was still higher than normal control group at 24h. HQSM could decrease the activity of CK and AST(P

Objective To observe the effect of comprehensive community intervention on cardiovascular risk factors in metabolic syndrome (MS) high risk group.Methods One hundred MS high risk patients were divided into intervention group (50 patients) and control group (50 patients) by random digits table.The intervention group accepted intervention on health education,health action,health habit and individual intervention on drug.The control group accepted natural intervention.The changes of waistcircumference,body mass index (BMI),blood pressure,fasting plasma glucose (FPG),lipids and serum uric acid (UA) were observed.Results In intervention group,the number of physical exercise,alimentary control and medication compliance increased 12％,16％ and 18％,and the number of smoking and alcohol drinking decreased 8％ and 6％.But in control group,the number of physical exercise,alimentary control,medication compliance increased 2％,0 and 4％,and the number of smoking and alcohol drinking decreased 4％ and 2％.There was significant difference (P＜ 0.05).Before treatment,the level of waist circumference,BMI,systolic pressure,diastolic pressure,FPG,total cholesterol,triacylglycerol,high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C) and UA between two groups had no significant difference (P ＞ 0.05).After 1 year's intervention,except HDL-C,the level of other index had significant difference between two groups (P ＜ 0.05 or ＜ 0.01).Conclusion Comprehensive community intervention can improve the status of obesity and dyslipidemia,and reduce blood pressure,plasma glucose and UA.

Objective Calcified epithelioma is a rare benign tumor in head and face. It is seldomly seen canceration. But it has local invasion behavior. The aim of this study was to explore the methodology in the diagnosis and treatment of calcified epithelioma in head and face, and to improve the accuracy of clinical diagnosis. Methods A retrospective analysis was carried out in 26 patients with calcified epithelioma in head and face. Results 53. 8 % patients with calcified epithelioma were less than 13-year-old. The disease was easily misdiagnozed. In this group of patients the misdiagnosis rate reached to 42. 3 %. Btype ultrasonographic diagnosis possessed a high level of concordance with histological diagnosis. There were three major types of calcified epithelioma: type 1, a mass with internal echoic foci; type 2, a hyperechoic mass with high beam; type 3, no calcification in the parenchyma. Piomatricoma was described as a skin tumour histologically composed of shadowcells, basophillic cells, foreign body giant cells and intracellular and stromal calcification. The treatment was excision and no recurrences were found by followup. Conclusions It is important for clinicians to know the clinical features of calcified epithelioma. Imaging studies including ultrasonography would help establish the diagnosis. Surgical excision with clear margins is recommended for this tumor.

Objective: To develop a narcissistic personality questionnaire for non-clinical Chinese people. Methods: The questionnaire was developed on the basis of data from open questionnaire, interview and Narcissistic Personality Inventory. Six hundred and twelve participants finished a Narcissism Questionnaire, Self-Esteem Scale, Trait Anxiety Inventory, and Satisfaction with Life Scale. Results: ①An exploratory factor analysis revealed three factors labeled authority, superiority, and selfadmiration; and a confirmatory factor analysis suggested that the goodness-of-fit indexes of this three-factor model was satisfactory; ②Cronbach alpha coefficients of narcissism questionnaire and its three factors ranged from 0.796 to 0.929; ③Consistent with previous studies, males were more narcissistic than females; ④Narcissism was pos- itively correlated with self-esteem, negatively correlated with anxiety and was not associated with satisfaction of life; selfesteem mediated the relationship between narcissism and anxiety. Conclusion: The self developed questionnaire illustrated satisfactory internal consistency reliability, construct validity, and criterion-related validity.

Objective To establish a 3D digitized visual model of the liver to provide anatomical structure for planning hepatic operations and creating accurate computer simulations of the liver.Methods Transverse sections of abdomen were chosen from the Chinese Visible Human dataset. The liver was reconstructed in three dimensions with surface rendering in combination with volume rendering reconstruction. Results Accurately segmented images of the main structures of liver were completed. The reconstructed structures could be displayed singly, in small groups or as a whole and could be continuously rotated in 3D space at different velocities. Conclusion The reconstructed liver is realistic and demonstrates the natural shape and exact position of hepatic structures. It provides an accurate model for the automated segmentation algorithmic study and a digitized anatomical model of the liver.

Objective To investigate clinically chromosome micro imbalance in children with unexplained mental retardation(MR) or development delay(DD) by using high resolution microarray comparative genomic hybridization(Array-CGH),to identify chromosome micro imbalance which might be associated with MR/DD,and evaluate the effectiveness of Array-CGH in etiological diagnosis of children with unexplained MR/DD.Methods One hundred and twenty-six children with unexplained MR/DD were recruited for this study by Array-CGH to detect chromosome micro imbalance.All chromosome micro imbalances were verified with database of genomic variation(DGV),Database of Chromosomal Imbalance and Phenotype in Humans using Ensembl Resources(DECIPHER) and literature review,to determine if the chromosome micro imbalances found in these children were associated with MR/DD.Results Twenty eight clinically relevant chromosome micro imbalances were detected among 26 children out of 126 children with unexplained MR/DD.The diagnostic yield for the MR/DD children was 20.6％ (26/126 cases).These chromosome micro imbalances were undetectable by chromosome analysis.All MR/DD children with chromosome micro imbalances had face dysmorphism and/or surface,organ dysmorphism.The most common abnormality was Prader-Willi syndrome/Angelman syndrome(3/26 cases,11.5％),which was followed by DiGeroge syndrome(2/26 cases,7.6％),Cri-du chat syndrome(2/26 cases,7.6％) and 16p11.2 deletion syndrome(2/26 cases,7.6％).Conclusions Chromosome micro imbalance is one of the most common causes of unexplained MR/DD.Array-CGH can detect disease associated with chromosome micro imbalance as a useful evaluation to help differential diagnosis of children with unexplained MR/DD.Screening for chromosome micro imbalance should be firstly carried out in those MR/DD children with face dysmorphism and/or surface,organ dysmorphism.

Object To develop a new method for the culturing of Gastrodia B1 in vitro, which may provide the theoretical basis of clonal propagation for its rapid reproduction Methods The small stem tubers and the stem buds were used as explants to culture in vitro under sterile conditions In the 1/2 MS medium containing 6 BA 1 mg/L, NAA 0 5 mg/L and banana 50 mg/L, the small stem tuber was induced to form protocorm In the 1/2 MS medium containing 6 BA 2 mg/L and NAA 0 2 mg/L, the stem bud was induced to form protocorm Results Each stem tuber formed a new protocorm within 50 d, which can be separated again to form rosette protocorm within 70 d The stem bud was cultured in vitro to form the protocorm within 140 d The protocorm bloomed within 160 d Conclusion The protocorm of G elata may be induced from the stem tuber and stem bud By subdividing these rosette protocorms a virtually indefinite clonal propagation can be achieved