Objective Microporous polysaccharide hemostatic powder ( MPPHP) is a most commonly used hemostatic mate-rial, but reports are rarely seen on its application in posterior spinal orthopedic surgery.The present paper aimed to investigate the val-ue of MPPHP in stopping bleeding in posterior spinal surgery. Methods Twenty-four 5-month-old New Zealand rabbits were equally randomized to an experimental and a control group.An 8 cm-long wound was made in the back muscle of animals to establish the model of spinal posterior surgery.MPPHP was applied to the wound of the experimental rabbits but none to that of the controls.Comparisons were made between the two groups of animals in the bleeding time, in vitro coagulation time, intra-operative blood loss, and results of pre-and post-operative blood routine and histopathological examinations. Results Statistically significant differences were observed between the experimental and control groups in the mean bleeding time ([15.36 ±1.42]s vs [347.58 ±32.43]s, P<0.05), in vitro coagulation time ([235.41 ±21.08]s vs [722.16 ±40.35]s, P<0.05), and intra-operative blood loss ([1.61 ±0.24]g vs [2.86 ± 0.37] g, P <0.05).After operation, the experimental animals showed a significantly higher count of erythrocytes than the controls ([5.68 ±0.46] ×106/L vs [4.47 ±0.43] ×106/L, P<0.05)and a higher level of hemoglobin than the latter ([12.67 ±1.18]g/L vs [10.64 ±1.05]g/L, P=0.00).There were no significant differences in the effectiveness and excellence rates of hemostasis between the experimental and control groups (100%vs 83.33%and 83.33%vs 66.67%, P>0.05).Pathological examination revealed mild bleeding and a small number of red blood cells in the muscle interstitium and blood vessel lumen of the experimental rabbits as compared with the controls. Conclusion Intraoperative topical ap-plication of MPPHP can reduce blood loss and achieve rapid and efficient hemostasis in posterior spinal surgery without affecting wound healing.