Infectious mononucleosis associated with Epstein-Barr virus infection (EBV-IM) is a proliferative disease of acute monocyte - macrophage system caused by primary EBV infection. Currently, the incidence IM has increased,and the cases are more severe. The mechanism of specific cellular immune response, new treatments and vaccines are increasingly becoming the focus.

Objective To analyze the short-term treatment effects and adverse effect on intermediate or advanced lung cancer by means of transbronchial arterial chemoembolization. Methods 68 patients were included in this study. First bronchial arteriography was performed to identify the tumor-feeding branch, and super-catheterize it. After CBP, EPI and 5-Fu was infused, embolization with gelatin sponge was carried out, chemoembolization was reperformed at 4~6 weeks intervals, 136 times of chemoembolization were done in 68 cases. Results The tumor blush of 51 patients stained obviously ,the feeding arteries with the second treatment decreased obviously. In 68 patients, the overall response rate was 82.3 %, the clinical beneficial rate was 97.0 %. The main side effect was myelosuppression, especially granulocytopenia, which was similar to that of conventional intravenous chemotherapy. No other severe adverse effect was seen. Conclusions Transbronchial arterial chemoembolization by selective tumor-feeding arteries catheterization has good short-term effect and clinic beneficial rate in the treatment of intermediate or advanced lung cancer, but the long-term treatment effects will be studied in the future.

Objective To study the refining of Radix Sophorae Flavescentis (RSF) decoction by microfiltration combined with ultrafiltration method,and to compare the refining effect with that refined by alcohol sedimentation method.Methods HPLC was applied to analyze the content of oxymatrine,ultroviolet (UV) spectrophotometry was used to determine the content of total flavonoids in RSF decoction,and suitable concentration of NaOH solution for the detergent were chosen in order to wash the membrane.Results The refining effect is better and the recovery ratio of efficient components is higher in RSF decoction refined by microfiltration combined with ultrafiltration method than in those refined by alcohol sedimentation method.Microfiltration combined with ultrafiltration method is of simple operation and short period,and ultrafiltration membranes can be easily washed.Conclusion The microfiltration combined with ultrafiltration method can be used as the alternative for the traditional alcohol sedimentation method in refining of RSF decoction.

Objective: To study the refinement of general flavone of sophora flavescens decoction by macroporous resins combined with ceramic microfiltration membranes (MR-CM), and compare it with macroporous resin combined with alcohol-sedimentation (MR-AS). Methods: UV-spectrophotometry method was applied to analyze the content of general flavone of sophora flavescens. Results: The porous ratio and effect of eliminating impurity of general flavone of sophora flavescens decoction by MR-CM method was higher than those by MR-AS method, but the MR-CM method is of simple operation and short period.Conclusion: MR-CM method is better than alcohol-sedimentation-macroporous resin method in refining of general flavone of sophora flavescens decoction.

ObjectiveTo evaluate the safety and efficacy of tirofiba in the treatment of patients with acute ST-elevation myocardial infarction (STEMI) undergoing emergency percutaneous coronary intervention (PCI). MethodsA total of 158 patients with acute STEMI were randomly divided into tirofiban group 1 (59 cases, received tirofiban before PCI), tirofiban group 2 (56 cases, received tirofiban when PCI) and control group(43 cases, only received PCI). The coronary reperfusion flow(TIMI grade) of infarct related artery (IRA) after PCI, the resolution of the sum of ST segment elevation(sum STR) at 90 min after the procedure, the changes of myocardial enzyme at 6 h and 12 h afterwards, the left ventricular ejection fraction (LVEF) 1 week later, the major adverse cardiac events(MACE) within 30 d, bleeding and thrombocytopenia complications were analyzed and compared among the three groups. ResultsTIMI reperfusion grades in tirofiban group 1[98.3％(58/59 )]and tirofiban group 2[92.9％(52/56)]were higher than those in control group[60.5％(26/43)](P ＜0.05). The resolution of sum STR at 90 min after PCI in tirofiban group 1 [(89.3 ± 6.9)％]and tirofiban group 2[(82.4 + 7.3)％]was higher than that in control group[(65.6 +8.1 )％](P＜ 0.01 ),and there was significant difference between tirofiban group I and tirofiban group 2 (P＜0.05 ). The occurrence of MACE within 30 d was lower in tirofiban group 1 and tirofiban group 2 than that in control group (P＜ 0.05). The level of CK-MB at 6 h and 12 h afterwards was lower in tirofiban group 1 than that in tirofiban group 2,and tirofiban group 2 was lower than control group (P＜ 0.05). LVEF 1 week later in tirofiban group 1[(56.2 + 6.4)％]was higher than that in tirofiban group 2[(51.1 + 4.9)％]and control group[(49.8 + 5.7)％](P ＜ 0.05),but there was no significant difference between tirofiban group 2 and control group (P ＞ 0.05). Although bleeding incidence in tirofiban group 1 and tirofiban group 2 was higher than that in control group, no severe bleeding and thrombocytopenia was observed. Conclusion Tirofiban can safely and effectively reduce the incidence of the ischemic events in the patients with acute STEM1 during preoperative of emergency PCI.

Objective To reduce the error caused by the non-linearity of headphone and device via the design of calibration algorithm for acoustic immittance.Methods Values of calibration chambers with different volumes (0.5,2.0 and 5.0 ml) were measured using the digital detection system.Then,the calibration data tables,for linearity calibration with inputting the values of acoustic immittance,were produced by means of processing the signal of acoustic immittance using the digital calibration algorithm based on the values from the calibration chambers,Results With the low frequency probe tone measurement,it was confirmed that the range of error among various calibration chambers with different volumes was 1％.Conclusion The calibration algorithm enables the improving of the detection accuracy and provides the reliable data for further clinical application.

Objective To develop a method for the determination of ganciclover in human plasma by RPHPLC.Methods Plasma containing ganciclover was extracted with methanol and methylene chloride,qualitative and quantitative analysis was carried out directly.Working curve,linear range,recovery,precision and so on was obtained according to the sample pre-processing method and analysis state.The HPLC method has been taken to investigate the plasma concentration of ganciclover for 12 volunteers.Results The relationship of the peak area of ganciclover concentration in plasma linear within the range of 0.05 μg/mL～1.60 μg/mL(r=0.9999).The lowest detection limit was 0.01 μg/mL(S/N≥13).The intra and inter-day RSD were less than 5.1%respectively.The recovery is about 90.0%～95.4%.Conclusion The established method in the article was shown to be sensitive,accurate and simple for the determination of ganciclover level.It is suitable for clinical detection of ganciclover and forensic medicine and toxicology analysis.

Objective:To investigate whether the increased plasma fibrinogen level is an independent predictor of cardiogenic embolism in patients with nonvalvular atrial fibrillation (NVAF).Methods:Ninety-eight patients with NVAF hospitalized for cardiac causes were evaluated,and 24 of them had a history of ischemic stroke.The relationship between fasting plasma fibrinogen level and ischemic stroke were analyzed.Results:After adjusting traditional cardiovascular risk factors.the patients with NVAF who had the history of ischemic stroke were significantly older than those without(mean [SD] age 73.6 [8.4]vs.69.1[10.3] years,P=0.017).1he prevalence of hypertension was significantly higher(83.3%va.66.2%,P=0.011).The proportion of patients with left ventricular systolic dysfunction was significantly higher(63.1% vs.45.9%,P=0.006),and plasma fibrinogen level increased significantly (mean [SD] 365 [108]vs.312[82] mg/dL,P=0.008).Conclusions:Plasma fibrinogen level is independently associated with a history of ischemic stroke in older patients with NVAF hospitalized for cardiac causes.

Objective To study HPLC fingerprints of Radix Glycyrrhizae from different habitats, develop the method of hierarchical cluster analysis by using the results of fingerprints, and discuss regional effect on accumulation of active ingredients of Radix Glycyrrhizae. Methods HPLC Fingerprint analysis method of active ingredients was developed and the method had been evaluated. Based on the standardized result of peak area of glycyrrhizin, liquiritin, liquiritigenin, isoliquiritin, and isoliquiritigenin, seventeen batches of Radix Glycyrrhizae were classified and identified using hierarchical cluster analysis. Results The methodological evaluation showed that this method had a good repeatability. The amounts of glycyrrhizin and flavones in Radix Glycyrrhizae from various habitats are different. Conclusion Accumulation of active ingredients of Radix Glycyrrhizae has some relativity with habitats.

Object To study the adsorption function of macroporous resin AB 8 for the purification of total flavones in Sophora flavescens Ait and the effects of concentration, pH, flow rate of the adsorbate and eluant Methods UV spectrophotometry was used to analyze the content of total flavone in S flavescens Results The appropriate adsorption conditions were: concentration of Sophora extract, 0 285 mg/mL ; pH=4; flow rate 3 BV/h, when 50% alcohol was used as eluant, the effect of desorption was satisfactory Conclusion AB 8 resin can be used to refine the total flavone in S flavescens