Objective To explore the effect of hydrocortisone sodium succinate on perioperative airway management in bronchial asthma patients. Methods 47 perioperative bronchial asthma patients with artificial airway in Linzhou City People Hospital were enrolled,and they were randomly divided into control group(24 cases) and therapy group(23 cases). Doxofylline 300 mg intravenous(IV)drip per day was given to the patients in both groups,and in therapy group,additionally hydrocortisone sodium succinate 500 mg IV drip per day was applied. The remission of asthma and changes in vital signs,arterial blood gas and respiratory function were observed in both groups. Results There were no significant differences in remission rate and invalid number at 30 minutes after treatment between therapy group and control group(both P>0.05). The remission rate of asthma in therapy group at 1 hour after treatment was significantly higher than that in control group(95.7%vs. 66.7%,P=0.023),the mean remission time was shorter than that in control group(minutes:38.09±15.93 vs. 45.83±18.75,P=0.012),the respiratory rate was lower(beats/min:20.8±2.3 vs. 22.3±3.3,P=0.042),and arterial partial pressure of oxygen〔PaO2(mmHg,1 mmHg=0.133 kPa):83.5±8.9 vs. 77.9±7.4,P=0.028〕,lactate〔Lac(mmol/L):1.87±0.29 vs. 2.09±0.33,P=0.029〕,forced vital capacity〔FVC(L):3.84±0.23 vs. 3.65±0.31,P=0.004〕and forced expiratory volume in 1 second〔FEV1(L):3.34±0.20 vs. 3.16±0.29,P=0.003〕were significantly increased compared with those in control group. But there were no significant differences in heart rate(HR),arterial oxygen saturation(SaO2),pH value,arterial partial pressure of carbon dioxide(PaCO2),FEV1/FVC,and peak expiratory flow (PEF)between the two groups(all P>0.05). Conclusion Hydrocortisone sodium succinate in conjunction with doxofylline can relax the symptom of perioperative bronchial asthma patients with artificial airway faster.

Objective To study the effects of eicosapentaenoic acid (EPA) combined with etoposide on the proliferation and apoptosis of human lung cancer cell lines A-549 in vitro.Methods The A-549 cells were cultured,respectively,with culture fluids containing 40 μg/ml EPA,10 μg/ml etoposide,40 μg/ml EPA + 10 μg/ml etoposide,20 μg/ml etoposide,or 40 μg/ml EPA + 20 μg/ml.The effects of EPA and/or etoposide on the proliferation of the cell line were detected by MTT assay.The morphological changes of the cells were observed using annexin V-fluorescein isothiocyanate/propidium iodine and acridine orange/ethidium bromide double staining under fluorescence microscope.The apoptosis and the changes of cell cycle were detected by flow cytometry.Results The inhibition rates of EPA plus etoposide groups on the proliferation of human lung cancer cells A-549 were significantly higher than those in other etoposide groups (all P ＜ 0.05).Cell staining showed that the amount of apoptotic cells in EPA plus etoposide groups was significantly larger than that in etoposide groups.The percentages of cells in S and G2/M phases of EPA plus etoposide groups were significantly higher than those of etoposide groups (all P ＜ 0.05).Conclusion EPA may enhance the effect of etoposide on inhibiting proliferation and promoting apoptosis for human lung cancer cells A-549.

Objective To investigate the effects of eicosapentaenoic acid (EPA) or EPA plus carboplatin on the proliferation and apoptosis of human lung cancer cell line A-549.Methods A-549 cells were cultured by 100 μg/ml carboplatin,80 μg/ml EPA,and their combination for 48 hours.MTT assay was used to determine the effect of EPA,carboplatin,or their combination on the proliferation of human lung cancer cell line A-549.The morphological changes of human lung cancer cell line A-549 were observed using HE staining,and apoptosis of A-549 cells was analyzed by flow cytometry.Results MTT assay showed that the proliferation inhibition rate of A-549 cells cultured with EPA plus carboplatin was 85.20％ ± 5.00％,which was significantly higher than that of cells cultured with 80 μg/ml EPA (32.85％ ± 3.00％,P =0.0001 ) or 100 μg/ml carboplatin (53.25％ ±3.00％,P =0.0013 ).HE staining showed apoptosis in all three groups,whereas the apoptosis rate reached 17.05％ ± 4.00％ in the combination group,which was significantly higher than that in carboplatin group (9.49％ ± 1.00％,P =0.0252).Conclusion EPA may enhance the effects of carboplatin in inhibiting the proliferation and promoting the apoptosis of human lung cancer cell line A-549.