OBJECTIVE:To observe the effect of Buflomedil Injection plus backwards traction and functional exercises on the hemorheology of patients with vertebral artery type of cervical spondylosis. METHODS:Sixty patients with vertebral artery type of cervical spondylosis were treated with 0.2 g Buflomedil Injection (diluted with 500 mL 0.9% Sodium Chloride Solution or 5% Glucose Solution,q.d by slow iv gtt for two courses of treatment with 7 days defined as 1 course) plus backwards traction and functional exercises. Patients' hemorheologic parameters including blood viscosity high shear rate,blood viscosity low shear rate,plasma viscosity,erythrocyte aggregation index,erythrocyte rigidity index,hematocrit,and fibrinogen etc were detected before and after treatment. RESULTS:Before treatment,all the hemorheologic parameters were significantly higher than the reference values of healthy adults(P

OBJECTIVE To explore the relationship between Ureaplasma urealytium(Uu),Mycoplasma hominis(Mh),and Chlamydia trachomatis(Ct) with infertilitas feminis.The drug sensitivity can offer evidence for rational usage to improve the cure rate.METHODS Of 320 cases of infertility womenthe,primary infertility accounted for 132 cases and the secondary infertility for 188 cases.Other 120 cases of normal women were chosen as control.Secretion samples were used to detect Uu,Mh,Ct,and drug sensitivity.RESULTS The rate of Uu,Ct,Uu+Ct,Uu+Mh in infertility group was significant higher than control group(P0.05);minocycline doxycycline,josamycin and erythromycin cyclocarbonte were sensitive to Uu+Mh;drug resistance rate was higher in Uu+Mh infection, multidrug resistance was very serious.CONCLUSIONS Uu,Mh and Ct infections are the major factors to infertilitas feminis.The detection of Uu,Mh and Ct is good for diagneosis.

BACKGROUND: Previous studies have demonstrated that the well combination of decellularized matrix and appropriate seeded cells could construct a tissue-engineered kidney.OBJECTIVE: To review advances in kidney tissue engineering and decellularized matrix.METHODS: The first author retrieved CNKI, Wanfang and PubMed databases for articles addressing kidney tissue engineering published from January 1996 to April 2016. The key words were decellularized matrix,extracellular matrix,tissue engineering, kidney, seeded cells in English and Chinese,respectively.RESULTS AND CONCLUSION: The decellularized matrix loses its immunogenicity due to the removal of cellular components, while it retains the important bioactive components of the extracellular matrix and the ultrastructure of the tissues and organs, making it more and more important in kidney tissue engineering. The decellularized matrix especially exerts an important role in the tissue-engineered construction of the entire kidney as driven by recently emerging all-organ acellular cell technology. Remarkable advances in kidney tissue engineering and decellularized matrix have been made in recent years, and realized the construction of a certain functional tissue-engineered kidney. However, there are still many challenges on the way to construct a completely functional tissue-engineered kidney.BACKGROUND: Previous studies have demonstrated that the well combination of decellularized matrix and appropriate seeded cells could construct a tissue-engineered kidney.OBJECTIVE: To review advances in kidney tissue engineering and decellularized matrix.METHODS: The first author retrieved CNKI, Wanfang and PubMed databases for articles addressing kidney tissue engineering published from January 1996 to April 2016. The key words were decellularized matrix,extracellular matrix,tissue engineering, kidney, seeded cells in English and Chinese,respectively.RESULTS AND CONCLUSION: The decellularized matrix loses its immunogenicity due to the removal of cellular components, while it retains the important bioactive components of the extracellular matrix and the ultrastructure of the tissues and organs, making it more and more important in kidney tissue engineering. The decellularized matrix especially exerts an important role in the tissue-engineered construction of the entire kidney as driven by recently emerging all-organ acellular cell technology. Remarkable advances in kidney tissue engineering and decellularized matrix have been made in recent years, and realized the construction of a certain functional tissue-engineered kidney. However, there are still many challenges on the way to construct a completely functional tissue-engineered kidney.

OBJECTIVE To understand the distribution and the drug resistance of the pathogenic bacteria so as to guide rational application of antibiotics for clinics.METHODS Bacteria culture and drug sensitivity with urine specimen were done among 1513 cases with urinary tract infection.RESULTS Urinary infection was mostly due to Gram-negative bacilli.Gram-positive cocci and fungi were the second.The top four pathogens were Escherichia coli(47.5%),Enterococcus faecium(8.7%),Candida albicans(7.0%),and Klebsiella pneumoniae(6.5%).The positive rates of urinary smear and culture were 51.9% and 29.3%,respectively with a coincidence rate of 77.4%.The drug resistance rates against imipenem,vancomycin and linezolid were zero,with 19.3% and 34.5% against extended-spectrum ?-lactamases-producing E.coli(ESBLs) and K.pneumoniae together,with 33.3% and 58.2% against meticillin-resistant coagulase negative Staphylococcus(MRCNS) and high-level gentamicin resistance(HLGR).CONCLUSIONS Urinary smear,bacteria culture and drug sensitivity tests can offer the basis for clinical rational use of antibiotics.

OBJECTIVE:To study the mycoplasma infection and the drug susceptibility of mycoplasma in cervical secretions from female genital tract in our hospital.METHODS:Ureaplasma urealyticum(Uu)and mycoplasma hominis(Mh)in cervical secretions sampled from 3 416 female genital tract inflammation cases in our hospital were identified by cultivation,and the antibiotic resistance was also detected.RESULTS:The total positive rate of mycoplasma was 43.7%;Uu infection accounted for 40.0%,which was sensitive to Cycloate erythromycin,Minocycline,Deoxycycline,Josamycin;Mh infection accounted for 0.7%,which was sensitive to Deoxycycline,Minocycline and Josamycin;Uu+Mh infection accounted for 3.0%,which was sensitive to Deoxycycline,Josamycin and Minocycline.CONCLUSION:Rational use of drugs based on drug susceptibility test is of importance to prevent the production of persister of mycoplasma.

OBJECTIVE:To study the value of bronchoalveolar lavage fluid(BALF) bacteria culture and drug sensitivity test in the hospital-acquired pneumonia(HAP) therapy.METHODS:The bacteria types in BALF samples and drug sensitivity test in 516 HAP cases were analyzed retrospectively.RESULTS:The positive rate of BALF culture was 46.9% with the main pathogenic bacteria of Candida albicans,Acinetobacter baumannii,Pseudomonas aeruginosa,Klebsiella pneumoniae.Of total nosocomial pneumonia,single bacteria infection accounted for 58.9% and mixed infection 41.1%.Candida had no drug resistance against 5-fluorocytosine,amphotericin B.Extended spectrum ? lactamase was found in 75.9% of Escherichia coli and 35.7% of Klebsiella pneumoniae with severe drug resistance.CONCLUSIONS:BALF bacteria culture and drug sensitivity test can provide reference for rational use of drug in the clinic.

Objective To compare the early outcomes of laparoscopic and open resection and evaluate the safety and feasibility of laparoscopic surgery in patients with colorectal cancer aged ≥ 70 years.Methods A total of 91 consecutive patients with colorectal adenocarcinoma underwent surgery in Yizheng City People's Hospital between Jan.2009 and Dec.2015.In 91 patients included in this study,38 received laparoscopic surgery and 53 underwent open surgery.Main outcome measures were clinical data,postoperative recovery status and short-term outcomes.Results There were no significant differences between two groups with respect to demographic indicators,clinicopathological results and chronic comorbidities had no significant difference between two groups.No death cases occured in both groups.One patient in the laparoscopic group required conversion to open surgery due to ureteral injury.Laparoscopic surgery was associated with significantly longer operating time [(238 ± 71.3) minutes vs (175 ± 60.8) minutes,P ＜ 0.001],less estimated blood loss [(145 ± 58 ml) vs (186 ± 45) ml,P ＜ 0.001)],a shorter postoperative hospital stay [(11.9 ± 3.9) days vs (14.5 ± 3.7) days],lower overall postoperative complication rate (23.7％ vs 45.3％,P =0.035),wound-related complication rate (2.6％ vs 22.6％,P =0.017) when compared with open surgery.Quality of surgical specimen,lymph nodes harvested were not significantly different between two groups.Conclusion Laparoscopic colorectal cancer surgery is safe and feasible in elderly patients,associated with better short-term outcomes when compared with open surgery.

Objective:To explore the clinical value of laparoscopic abdominoperineal resection(LAPR) with pelvic peritoneum closure for patients with low rectal cancer.Methods:The clinicopathological data of 90 patients with low rectal cancer who underwent laparoscopic abdominoperineal resection from Mar 2014 to Jan 2019 at the Subei People's Hospital of Jiangsu Province were retrospectively analyzed. These patients were divided into closed pelvic floor peritoneum group (study group, n=42) and without pelvic floor peritoneum group (control group, n=48) . Results:The postoperative hospital stay of the study group was shorter than that of the control group[(10.8±3.0) d vs. (12.4±3.1) d, t=2.569, P=0.013]. There was no statistically significant difference in the operation time , intraoperative blood loss , time to first flatus ,first time of getting out of bed between the two groups. Perineal incision infection and perineal incision dehiscence occurred in 2 cases and 1 case in the study group, and 10 cases and 9 cases in the control group respectively (χ 2= 5.007, P=0.025; χ 2=6.077, P=0.033). In the study group, there were 0 cases of perineal hernia, 1 case of pelvic floor peritoneal hernia and 2 cases of adhesive intestinal obstruction, while those in the control group were 7 cases, 8 cases and 9 cases, respectively (χ 2=6.642, P=0.013; χ 2=5.079, P=0.033; χ 2=4.085, P=0.043). Conclusion:Laparoscopic abdominoperineal resection with pelvic peritoneum closure significantly reduces the incidence of postoperative perineal-related complications and shorten postoperative hospital stay.

BACKGROUND:Orthopedic osteotomy at the apex of kyphosis is best for treatment of ankylosing spondylitis from a biomechanical aspect, but there is a high risk for intraoperative spinal cord injury.
OBJECTIVE:To explore the clinical efficacy of vertebral plate osteotomy+vertebra osteotomy+long-segment pedicle screw fixation in the treatment of ankylosing spondylitis with kyphotic deformity.
METHODS:Thirty-six patients with ankylosing spondylitis were subjected to pedicle subtraction osteotomy and Smith-Peterson osteotomy, and then fol owed up for 3 months to 2 years.
RESULTS AND CONCLUSION:After implantation, sagittal imbalance did not occur in 36 patients, and the improvement rate of sagittal imbalance was 64%. The improvement rates of thoracolumbar kyphosis and chin-brow vertical angle were 60%and 98%, respectively. The pain relief rate was 64%, and the Oswestry Disability Index was 95%. There were no pul ed nails, broken nails and broken robs after implantation. These findings indicate that the combination of selective osteotomy technique and long-segment internal fixation can achieve stable fixation effects, prevent sagittal imbalance, and avoid the occurrence of pul ing nails, breaking nails and breaking robs caused by osteoporosis.

OBJECTIVE: To identify and analyze drug sensitivity of Corynebacterium glucuronolyticum iscolated from clinic, and to provide reference for clinical drug use. METHODS: Two strains isolated from the urine specimens of urolithiasis-induced urinary tract infection patients in our hospital were inoculated into Columbia blood plate and the MacConkey plate. The growth of strains was observed and counted. Protein mass spectrometry of strains was detected by MALDI-TOF-MS. DNA of strains was extracted, and PCR was used to amplify the 16S ribosome RNA (rRNA) sequence. Bi-directional sequencing of 1 500 bp target bands was conducted. Blast comparison between it and GenBank database was conducted to identify bacterial strain. Drug resistance of 2 strains was monitored by Etest assay. RESULTS: Two strains grew on the Columbia blood plate (with colony forming unit ＞105 CFU/mL) and did not grow on the MacConkey plate. Two strains were Gram-positive Corynebacterium and showed palisading or eight type arrangement. Two strains were C. glucuronolyticum by MALDI-TOF-MS identification, with reliability of 99. 9％. The characteristic peaks of m/z 2 431, 3 089, 3 364, 3 378, 4 200, 5 508, 6 302, 6 637, 6 730, 6 946, 12 603 appeared. Blast comparison showed that the sequence homology of 2 strains compared with C. glucuronolyticum strain known in GenBank were higher than 98 ％. Results of drug sensitivity test showed that strain 1 was resistant to ceftriaxone and ciprofloxacin, and sensitive to 14 other antibiotics as penicillin G; strain 2 was resistant to ceftriaxone, erythromycin, ciprofloxacin, tetracycline and clindamycin, moderately sensitive to cefotaxime, and sensitive to 10 other antibiotics. CONCLUSIONS: Two strains are C. glucuronolyticum, and drug resistance of them to commonly used antibiotics is different. The strains are rare pathogen of urinary tract and show multidrug resistance. Antibiotics should be selected according to the results of strain identification and drug sensitivity test.