This study was designed to determine the protective effect of scopolamine and catalase on the arterial endothelial cells during reoxygenation. The culture of endothelial cells from bovine aorta subjected to ischemia for 2 hours and reoxygenation for 30 minutes was used as a model for inves-tigaion. In the experimental group, scopolamine was added to the arterial endothelial cells culture medium, Hanks solution containing no sugar or oxygen; or in the control group no medicine was added to the ordinary Hanks solution. The alteration of glutathione ( GSH ) and malondialdehyde (MDA) of endothelial cells, and nitric oxide (NO) and lac-tate dehydrogenase (LDH) in the supernatant fluid were determined. The results demonstrated the following changes: (1) GSH in the control group significant reduction from the preischemia value 44. 5 + 2.45 to postischemia and reoxy genation value 35.08 + 5.72ug/mg protein, vs the post-ischemia and reoxygenatior value of 46. 67+3.20ug/ mg protein (scopolamine) and 43.38+2.98ug/mg protein (catalase). The differences between the GSH changes of control group and medicine treated group are statistically significant (P

OBJECTIVE:To observe the effect of Buflomedil Injection plus backwards traction and functional exercises on the hemorheology of patients with vertebral artery type of cervical spondylosis. METHODS:Sixty patients with vertebral artery type of cervical spondylosis were treated with 0.2 g Buflomedil Injection (diluted with 500 mL 0.9% Sodium Chloride Solution or 5% Glucose Solution,q.d by slow iv gtt for two courses of treatment with 7 days defined as 1 course) plus backwards traction and functional exercises. Patients' hemorheologic parameters including blood viscosity high shear rate,blood viscosity low shear rate,plasma viscosity,erythrocyte aggregation index,erythrocyte rigidity index,hematocrit,and fibrinogen etc were detected before and after treatment. RESULTS:Before treatment,all the hemorheologic parameters were significantly higher than the reference values of healthy adults(P

OBJECTIVE:To study the mycoplasma infection and the drug susceptibility of mycoplasma in cervical secretions from female genital tract in our hospital.METHODS:Ureaplasma urealyticum(Uu)and mycoplasma hominis(Mh)in cervical secretions sampled from 3 416 female genital tract inflammation cases in our hospital were identified by cultivation,and the antibiotic resistance was also detected.RESULTS:The total positive rate of mycoplasma was 43.7%;Uu infection accounted for 40.0%,which was sensitive to Cycloate erythromycin,Minocycline,Deoxycycline,Josamycin;Mh infection accounted for 0.7%,which was sensitive to Deoxycycline,Minocycline and Josamycin;Uu+Mh infection accounted for 3.0%,which was sensitive to Deoxycycline,Josamycin and Minocycline.CONCLUSION:Rational use of drugs based on drug susceptibility test is of importance to prevent the production of persister of mycoplasma.

OBJECTIVE:To study the value of bronchoalveolar lavage fluid(BALF) bacteria culture and drug sensitivity test in the hospital-acquired pneumonia(HAP) therapy.METHODS:The bacteria types in BALF samples and drug sensitivity test in 516 HAP cases were analyzed retrospectively.RESULTS:The positive rate of BALF culture was 46.9% with the main pathogenic bacteria of Candida albicans,Acinetobacter baumannii,Pseudomonas aeruginosa,Klebsiella pneumoniae.Of total nosocomial pneumonia,single bacteria infection accounted for 58.9% and mixed infection 41.1%.Candida had no drug resistance against 5-fluorocytosine,amphotericin B.Extended spectrum ? lactamase was found in 75.9% of Escherichia coli and 35.7% of Klebsiella pneumoniae with severe drug resistance.CONCLUSIONS:BALF bacteria culture and drug sensitivity test can provide reference for rational use of drug in the clinic.

OBJECTIVE To explore the relationship between Ureaplasma urealytium(Uu),Mycoplasma hominis(Mh),and Chlamydia trachomatis(Ct) with infertilitas feminis.The drug sensitivity can offer evidence for rational usage to improve the cure rate.METHODS Of 320 cases of infertility womenthe,primary infertility accounted for 132 cases and the secondary infertility for 188 cases.Other 120 cases of normal women were chosen as control.Secretion samples were used to detect Uu,Mh,Ct,and drug sensitivity.RESULTS The rate of Uu,Ct,Uu+Ct,Uu+Mh in infertility group was significant higher than control group(P0.05);minocycline doxycycline,josamycin and erythromycin cyclocarbonte were sensitive to Uu+Mh;drug resistance rate was higher in Uu+Mh infection, multidrug resistance was very serious.CONCLUSIONS Uu,Mh and Ct infections are the major factors to infertilitas feminis.The detection of Uu,Mh and Ct is good for diagneosis.

0.05). CONCLUSIONS Minocycline,deoxycycline and josamycin can be chosed to cure Mycoplasma infection in this territory. Drug fast rate of mycoplasma is changing with the time. It is important for guiding clinic to monitor drug resistance of mycoplasma of Genitourinary tract.

OBJECTIVE To understand the distribution and the drug resistance of the pathogenic bacteria so as to guide rational application of antibiotics for clinics.METHODS Bacteria culture and drug sensitivity with urine specimen were done among 1513 cases with urinary tract infection.RESULTS Urinary infection was mostly due to Gram-negative bacilli.Gram-positive cocci and fungi were the second.The top four pathogens were Escherichia coli(47.5%),Enterococcus faecium(8.7%),Candida albicans(7.0%),and Klebsiella pneumoniae(6.5%).The positive rates of urinary smear and culture were 51.9% and 29.3%,respectively with a coincidence rate of 77.4%.The drug resistance rates against imipenem,vancomycin and linezolid were zero,with 19.3% and 34.5% against extended-spectrum ?-lactamases-producing E.coli(ESBLs) and K.pneumoniae together,with 33.3% and 58.2% against meticillin-resistant coagulase negative Staphylococcus(MRCNS) and high-level gentamicin resistance(HLGR).CONCLUSIONS Urinary smear,bacteria culture and drug sensitivity tests can offer the basis for clinical rational use of antibiotics.

This study was aimed to build up a diagnosis and treatment procedure of traditional Chinese medicine (TCM) for HIV/AIDS headache. Domestic and foreign articles correlated to HIV/AIDS headache diagnosed and treat-ed by TCM were summarized. The specialist questionnaire of clinical diagnosis and treatment standard operating pro-cedures of TCM for HIV/AIDS headache was designed by focus group discussions. And the national specialist ques-tionnaire survey was carried out twice. The results showed that the standard operating procedure of TCM clinical di-agnosis, treatment, nursing and therapeutic efficacy assessment for HIV/AIDS headache was preliminarily established. It was concluded that this regulation identified concept, etiology and pathogenesis of HIV/AIDS, established TCM standard diagnosis and treatment service. It also demonstrated features of propaganda and education, follow-ups, con-secutive diagnosis and treatment inside or outside the hospital.

This study was aimed to establish traditional Chinese medicine (TCM) diagnosis and treatment procedure of varicella zoster virus (VZV) in AIDS, and to construct questionnaires for key points and revision of the procedure. The TCM draft of diagnosis and treatment procedure for VZV in AIDS was established through literature retrieval and peer review. Two rounds of surveys were carried out to investigate the confirmation and advice of in-group specialist to key points of TCM or integrative medicine draft including diagnosis, treatment and nursing. Then, diagnosis and treatment procedure were revised according to survey results. The results showed that the recovery of complete ques-tionnaires in the first-round survey was 96%. More confirmation of specialists were given to concept, clinical feature, cause, mechanism, case history and general examination, diagnostic criteria, syndrome differentiation and treatment of dampness-heat of liver channel syndrome, skin, dietary and psychological nursing, treatment course and therapeutic effect standard. The coefficient of variations (CVs) of experiential effective recipe, moxibustion and massage, auxiliary examination, syndrome differentiation and treatment of dampness stagnancy due to spleen deficiency syndrome and qi stagnation and blood stasis syndrome, and western medicine treatment were large. The weight coefficients of all items were within 0.043 6 and 0.046 2. The Cronbach Coefficient Alpha (CCA) was 0.996 and the split-half reliability R was 0.86. Recovery of complete questionnaires in the second-round survey was 100%. More confirmation of special-ists were given to outline, cause, mechanism, case history and general examination, diagnostic criteria, syndrome dif-ferentiation and treatment of three syndromes, experiential effective recipe, skin, dietary and psychological nursing. The CV was 0. The CV of auxiliary examination, moxibustion and massage was 0.063 8, which was less than those of first-round survey. The consistency of specialist was relatively increased. The weight coefficients of all items in the second-round survey were within 0.058 2 and 0.059 0. The CCA was -0.041 and the split-half reliability R was 0.79. A new revised procedure was preliminarily established according to results of two rounds of surveys. It was concluded that the activeness, concentration and coordination of specialists were good in two rounds of surveys. Con-sensus in key points of the procedure draft was reached including diagnosis, treatment and nursing.

OBJECTIVETo study the optimum storage methods of Pinellia ternata from Sichuan.

METHODNine methods were used to overwintering storage of the seed-stem of P. ternata from Sichuan. The germination of seed-stem was observed through the following year after planting, then the germination percentage, vigor of germination and germination index were calculated. At the seedling stage, the physiological indexes like rooting number, root length, root activity, chlorophyll content and catalase activity etc. were determined.

RESULTUnder the different storage conditions, all the tested indexes of P. ternata from Sichuan were significantly different between each other (P < 0.05), except the chlorophyll content.

CONCLUSIONThe seed-stem of P. ternata from Sichuan should be preserved in the wet sand (or in soil) in a greenhouse or 4 degrees C refrigerator. Under these conditions, the germinating vigor and growth activity of seed-stem performed the best.

Agriculture ; methods ; Germination ; Pinellia ; growth & development ; physiology ; Plant Stems ; growth & development ; Seeds ; growth & development