High flow nasal cannula oxygen therapy (HFNC) is a new type of oxygen therapy. Because of its unique physiological effects, the clinical application is becoming more and more popular. The article summarized HFNC has obvious advantages in oxygenation and comfort. In the clinical, HFNC is widely used in adult patients with acute hypoxemic respiratory failure, respiratory failure after the operation or extubation and respiratory tract invasive operation. But there is no literature reports in improving ventilation to reduce carbon dioxide. If patients′ respiratory, cycle and oxygenation show no significant improvement after application of HFNC for a short time, it should be replaced as soon as possible for the noninvasive and invasive mechanical ventilation. In this article, the characteristic and application of HFNC will be reviewed.

Objective To study the mechanism of dermal fibroblasts as a feeder layer to support the growth of human keratinocytes. Methods Human dermis fibroblasts were isolated and cultured and then treated with mitomycin-C. The expression of type Ⅰand type Ⅲ precollagen mRNA and relevant protein in feeder layer were examined by RT-PCR and Immunohistochemistry. KCs were cultured both on FB and NIH3T3 feed layer as control, the adhering numbers and the time of fusion were recorded. Results RT-PCR showed an increase of type Ⅰprecollagen mRNA in FB feeder layer as compared with that of normal fibroblasts (P