Objective To investigate the effect of using evidence - based nursing (EBN) in the course of nursing patients with severe craniocerebral injury combined pulmonary diseases. Method Divided 160 patients into research group and control group randomly, there were 80 patients in each group. Using EBN in the research group, and routine nursing measures was used in the control group, and then compare the rehabilitation of patients in 2 groups. Result The condition of rehabilitation in research group was significant better than that of in control group, P

Laser photocoagulation,intravitreal injection of antibody against vascular endothelial growth factor (VEGF) or corticosteroids and pars plana vitrectomy are current popular therapeutic approaches for diabetic retinopathy (DR).However,some DR patients still progress to irreversible blindness even after the above treatments which do not aim at the pathological mechanisms and influence factors for DR.Thus,with the further elucidation on the molecular pathological mechanisms and overall understanding of the factors affecting DR development,more and more potential therapeutic interventions such as neuron protection,vascular reconstruction and protection,gene therapy,non-VEGF dependent antineovascularization agents have been explored.Individual precise therapy based on the potential therapeutic targets would provide the promising future for DR patients.

Objective To summarize the prognosis of elderly patients with colorectal cancer excision who were divided into different groups by the nutritional status before their surgery.Methods In our hospital,100 patients (age from 64 to 70)with colorectal cancer were performed the screening of nutritional risk by NRS2002 before surgery.Patients with score from 1 to 2 were as the group A,and patients with score over 3 were as group B.The related indexes and recovery between two groups were compared.Results The NRS2002 rating scale score showed that 76 patients were at nutritional risk,37 cases among them received nutrition support and had faster recovery of intestinal function the time of tube pulled out after operation[(1.8 ±0.4)d vs.(2.9 ±1.5)d],exhaust time[(2.4 ±0.6)d vs.(4.3 ±0.9)d],time of in-take food[(2.9 ±0.5)d vs.(4.5 ±1.1)d],length of stay[(10.8 ±0.9)d vs.(14.1 ±1.3)d],incidence of infection related complica-tions (0 vs.5.13%),the differences were statistically significant(P <0.05).Conclusion Problems exist in varying degrees of loss of nu-trition in elderly colorectal cancer patients before surgery,an objective assessment of the extent and targeted care and nutritional support can significantly improve the postoperative nutrition and to facilitate recovery of gastrointestinal function after surgery.

Objective To investigate the effects of propofol on P2X7 receptor activition and IL-1β production induced by endotoxin in murine RAW264.7 macrophages. Methods RAW264.7 macruphages were treated with LPS (1 μg/ml) for 4 h to induce the production and release of IL-1β, and pretreated with BBG (specific P2X7 receptor antagonist) 1 μmol/L or propofol 1-100 μmol/L for 20 min before LPS stimulation, and IL-1β release was measured using ELISA kit. Whole-cell patch clamp technique was used to record the P2X7-gated currents induced by 1 mmol/L ATP, the cells were exposed to propofol with 1-1 000 -μmol/L for 4 min, and the IC_(50) level of propofol was achieved. Western blot technique was used to measure the production of pro-lL-1β protein and IL-1β protein intracellularly after LPS treatment for 4 h under different concentrations of propofol. Results IL-1β was released from RAW264.7 macrophages after LPS stimulation, which was decreased by propofol, and the IC_(50) level of propefol was (24±3) μmol/L. P2XT-gated currents were inhibited by propofol, and the IC_(50) level was (33±5) μmol/L. Pro-IL-1β protein intracellularly was up-regulated after LPS stimulation, and propofol with 3-100 μmol/L decreased the up-regulation of pro-IL-1β intracellularly induced by LPS. Conclusion Propefol could inhibit IL-1β release from RAW264.7 macrophages treated by LPS, which is mediated by inhibiting P2X7 receptor activition and decreasing the production of pro-IL-1β intracellularly.

Ca2+ activity has been found to associate with the immunity and inflammation within cardiovascular diseases in recent years. Researchers have begun to focus on the effect of calcium channel blockers, which could modify the immunity and inflammation. This review presented the mechanism underlying the concentration and activation of Ca2+ influenced the response of immunity and inflammation and how calcium channel blockers interfered with it, which may have potential in treatment of cardiovascular diseases.

Objective To investigate the relationship between angiotensin 1-converting enzyme(ACE)inserting/defaulting(I/D)gene polymorphisms and the carotid intima-media thickness(CCA-IMT)in patients with type 2 diabetes(T2DM).Methods The polymorphisms of ACE(I/D)was determined by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)method and the CCA-IMT was assessed using non-invasive high resolution B-mode ultrasonography in 120 T2DM in Han's population of Dongguan city.The data were analyzed by Logistic regression to explore the independent and significant risk factors for CCA-IMT increase in type 2 diabetic patients.Results The DD genotype were significantly more common in diabetic patients with CCA-IMT increase than in those without it(F=10.164,P=0.006);Logistic regression analysis showed that DD genotype,age,male gender and essential hypertension contributed significantly to the CCA-IMT increase in type 2 diabetic patients(OR=3.689,1.054,1.562,respectively,P

Nuclear receptors(NRs)superfamily signaling plays an important role in regulating the expression of gene involved inenergy homeostasis.The nuclear receptors regulate target gene expression through the recruitment of coregulatory proteins,which act as scaffolds or plateforms for linking NRs with enzyme complexes that modify DNA and histones.As a transcriptional co-repressor for NRs,RIP140 negatively regulates the transcription of target gene in metabolic tissue,such as adipose tissue,muscle and liver.In the absence of RIP140,genes expressions involved in metabolic pathways were upregulated including glycolysis,triglyceride synthesis,TCA cycle,fatty acid ? oxidation,mitochondrial electron transport and oxidative phosphorylation.RIP140 may be a candidate therapeutic target for metabolism syndromes.

Objective:To explore the effects of diltiazem on ventricular remodeling and inflammation in rat heart following acute myocardial infarction(AMI).Methods:The model of AMI rats was randomly divided into diltiazem group(D group)and control group(AMI group),besides another group of sham operation(S group).The data of ejection fraction(EF) and the left ventricular mass(LVM)were examined with echocardiography,and leukocyte infiltration in situ was analyzed on the HE staining slices,with the expression of proinflammatory cytokines(IL-I?,IL-6,TNF-?)detected by RT-PCR at 1d,3d,1w,2w and 4w intervals after AMI.Results:The results from echocardiography showed that EF increased(73.7?3.1% vs 61.0?2.6%)and LVM decreased(0.81?0.12g vs 0.92?0.12g),both significantly in D group at 4w,compared with those of the AMI group(P

Objective:To compare the healing of gingiva after electrosurgical gingivectomy with different power outputs and electrodes. Methods:15 rabbits were randomly assigned to 3 groups which used different electrodes: needle electrode, long loop electrode and loop electrode. 1 animal was chosen as control in each group. Free gingiva of anterior teeth had been removed 2 mm in height with different power output and electrosurgical knife. Gingival specimens were harvested for histopathological study on days 1,7,14,and 21 after operation. Statistical analysis was performed on wound healing. Results:Electrical knife with medium power output caused significantly higher degree of inflammation than other techniques(P0.05). Conclusion:When electrosurgery is used on gingiva, there is no significantly difference with different electrodes. Longer observation is needed after gingival electrosurgery with medium power output electrical knife.