Objective Observe the clinical effect of the controllable airbag elastic chest strap after the breast cancer operation,studying the clinical value of the controllable airbag elastic chest strap.Methods Between May.2009 and Oct.2012,71 women having surgery for breast cancer at The Affiliated Second Hospital of Nan Hua University Oncology Center.They were to receive either a common thoracic girdle compressed binding(n =35),or the controllable airbag elastic chest strap compressed binding (n =36) at the postoperation.The following complications were evaluated:the incidence of seroma formation and duration,the incidence of skin flap swelling,and the dressing time.Results Thirty-five patients with common thoracic girdle is 14 cases of subcutaneous effusion,fluid rate was 40.0％,the effusion duration 7 to 31 days,an average of 17.6 ±7.3 days,15 cases with skin redness (disc),swelling rate is 42.9％,the average treatment time 18.7 ±3.5 minutes; With the controllable airbag elastic chest strap group of six cases of subcutaneous effusion,fluid rate was 16.7％,the duration of effusion 5-14days,an average of 10.5 ± 3.9 days,skin redness (disc),swelling rate is 13.9％,the average treatment time 12.2 ± 2.4 minutes.Conclusions The controllable airbag elastic chest strap can reduce subcutaneous effusion,reduce skin flapswelling,saving the treatment time,is worth promoting in the clinical medical supplies.

The ultraviolet spectrophotometric method for determination of vitamin A in breast milk was simplified. The milk sample was extracted directly with ammonia water, ethyl alcohol and ether, the saponification procedure be- ing omitted. The optimal height of the aluminum oxide adsorption column and the optimal rate of flow for elution were developed. The observation was made whether the elution for vitamen A was complete by identifying the fluorescence of vitamin A directly under ultraviolet light in the eluat-es collected instead of SbCl3-Vitamin A blue color reaction. The simultaneous estimation of Vitamin A in breast milk and in the standard solution of Vitamin A with this method showed E300nm/E325nm less than 73%, agreeable with the British Pharmacopoeia. The recovery was 100.8%. This procedure has advantages of high sensitivity, simplicity, time saving and economy.

Objective To investigate the clinical efficacy of acupuncture and massotherapy for cerebral palsy-salivation. Method Forty-eight patients with infantile cerebral palsy were randomly allocated to two groups. The control group of 24 patients received acupoint application of Chinese herbal medicine and did the tongue exercises. The observation group of 24 patients received acupuncture and massotherapy in addition. Result Of the control group, five patients were assessed as grade Ⅰ, four patients as grade Ⅱ, 10 patients as grade Ⅲ, two patients as grade Ⅳ and three patients as grade Ⅴ in the TDS grading after treatment. Of the observation group, 10 patients were assessed as grade Ⅰ, seven patients as grade Ⅱ, four patients as grade Ⅲ, two patients as gradeⅣ and one patients as grade Ⅴ in the TDS grading after treatment. The TDS grading assessment was better in the observation group of patients than in the control group. After treatment, middle cerebral artery blood flow velocity and vessel pulsation index were (135.64±13.72)cm/s and (1.05±0.17), respectively, in the control group and (144.78±16.45)cm/s and (0.90±0.18), respectively, in the observation group. There was no statistically significant difference in speech training between the two groups (P>0.05). The number of independent eating patients was significantly larger in the observation group than in the control group; there was a statistically significant difference (P<0.05). Fine action, adaptive behavior and personal social behavior development levels were significantly higher in the observation group than in the control group; there was a statistically significant difference (P<0.05). Both groups of patients could tolerate the therapeutic dosage and course and had no severe adverse reactions. There was no statistically significant difference in safety grade between the two groups (P>0.05). Conclusion Acupuncture and massotherapy is safe and of a fair effect in treating cerebral palsy-salivation. It can increase cerebral blood flow, promote the contraction of local muscles, control salivation and improve the quality of life in cerebral palsy patients.

Objective To observe the effects of electric stimulation on mastoidea in the patients with ischemic stroke by transcranial Doppler sonography (TCD) and brainstem auditory evoked potentials (BAEP).Methods 40 patients with ischemic stroke were equally divided into two groups, i.e., observation group (20 cases) and control group(20 cases). The two groups received routine treatment, while the observation group added electrical stimulation on mastoidea. The blood flow vecolity of anterior cerebral artery (ACA), middle cerebral artery (MCA), posterior cerebral artery (PCA), vetebral artery (VA) and basilar artery (BA), and the peak latency(PL) of wavesⅠ, Ⅲ and Ⅴ, and inter-peak latency(IPL) of Ⅰ-Ⅲ and III-V were examined by TCD and BAEP before and 2 weeks after treatment.Results The blood flow velocity in the observation group accelerated after treatment compared to pre-treatment(P<0.05). Compared with the control group, the blood flow velocity in the observation group accelerated more markedly(P<0.001). The nerve conduction in both groups improved after treatment. After treatment, PL of waves I, Ⅲ and V, and IPL of I-Ⅲ, III-V and III-V/I-Ⅲ in the observation group improved more significantly than the control group(P<0.05). Conclusion Electric stimulation on mastoidea can enhance the blood flow velocity of brain and the function of brainstem nerve conduction in patients with ischemic stroke.

Objective To establish a method to evoke cerebral potentials by stimulating nociceptive fibers with contact heat evoked potential stimulator,evaluate the state of nociceptive system in patients with multiple sclerosis and assess the value of the potentials in multiple sclerosis(MS).Methods Thirty-six definite MS patients and 40 sex-,height-and aged-matched healthy persons underwent stimulation of contact heat delivered via a circular thermode to excite selectively nociceptors with a rapid rising time at 70℃/s to elicit pain and contact heat evoked potentiaI(CHEP).Thermal stimuli were sent at two intensity levels (47 ℃ and 51℃)to 3 body sites:volar surface of the forearm,the skin of leg 5 cm proximal to the medial malleolus and lumbar part.The CHEP were recorded from Cz.The relationship between the stimulus intensity and pain rating was observed,and the main components of the evoked potential were recorded.Then,somatosensory evoked potential(SEP)was examined in 36 patients with MS.Results CHEP were elicited reliably and stably in all control subjects.In contrast,in 4 patients there were no recordable CHEP on stimulation of the upper limb,and in some cases of lower limb(n=5).Conduction velocity of Aδ fihers was(18.1±7.3)m/s.The 21 MS cases had hypesthesia in upper limb and 29 cases in lower limb.The visual analog scale(VAS)for pain perception was higher in control subjects(upper limb:8.0±0.7;lower limb:7.9±0.7)than MS with hypesthesia(upper limb:6.1±0.9;lower limb:5.6±1.3,Z=-3.249 and -5.272,both P＜0.01).The group of patients (MS) with hypesthesia(upper limb 17 cases,lower limb 24 cases)had markedly reduced N-P amplitudes(upper limb:(30.5±12.8)μV;lower limb:(28.2±16.2)μV,t=-4.612 and -3.144,both P＜0.01)and prolonged N-wave latencies(upper limb:(387.3±34.2)ms;lower limb:(489.9±70.2)ms,t=4.790 and 4.798,both P＜0.01)compared with the control group in CHEP mediated by Aδ fibers.CHEP abnormality was observed more often in the lower(26/36,72.2%)than the upper limb(16/36,44.4%,P=0.031)and SEP(19/36,52.8%,χ~2=4.261,P=0.039).CHEP were abnormal in 3 of 15 skin areas with clinically normal nociception in upper limb,and in some cases of lower limb(2 of 7).Conclusions CHEP provides a clinically practical,non-invasive and objective measure,and can be a useful additional tool for the assessment of nocieptive system.Combined assessment of other Eps can help to document dissemination of demyelinating CNS lesions and detect subclinical lesions thus contribute to the diagnosis of multiple sclerosis.

BACKGROUND: Fiber post is widely used in front teeth aesthetic repair, but some defect teeth cannot form ferrule or thick root canal, which resulted in fiber post prosthetic replacement defluxion and a failure of repair. Presently, new-style resin cement had self sticking component, showing good effects in sticking of fiber post. Whether combination of new-style resin cement can repair affected teeth using fiber post deserves further investigation.OBJECTIVE: Through fatigue test, residual flexural strength and scanning electron microscope (SEM) observation to compare the strength of severely weakened roots restored with three resin cements. METHODS: Eighteen intact maxillary central incisors were collected and formed to severely weakened canals at the same size. Three kinds of resin cements were used to restore. Rely X Unicem, Panavia F and Super-Bond C&B resin cement and identical glass fiber post were used. All the specimens were restored using Ni-Cr ceramic crown, and placed on TCML chewing machine that loaded 1 200 000 cycle forces, and the cycle times when failure occurred were recorded. The samples were intact following loading received residual flexural strength testl SEM was employed to observe breakage surface of the samples. RESULTS AND CONCLUSION: Mean cyclic loading was significantly greater in the Super-Bond C&B Group (1 200 000 times) than in the Relyx Unicem Group (640 000 times) and Panavia F Group (550 000 times) (P< 0.05). No significant difference was detected between the Rely X Unicem and Panavia F Groups. Residual flexural strength was 747.99 N in the Super-Bond C&B Group. SEM demonstrated that Super-Bond C&B formed longer resin processes, which were more than other two groups. Results confirmed that Super-Bond C&B demonstrated better results when restoring flared roots with prefabricated glass fiber posts.

This article reviewed the researches proceeding on the contamination and detection of the foodborne pathogen noroviruses (NoVs) in fresh produce, which involved the NoVs contaminations in fresh produce, the special attachment of NoVs in fresh produce, the NoVs outbreaks associated with fresh produce and the NoVs detection in fresh produce. There had been an increase in reported infectious disease risks associated with the consumptions of fresh produce for recent 30 years. Because the NoVs, as a primary cause of viral gastroenteritis thoughout the world, were highly contagious, had a low infectious dose, and were persistent in the environment. And also the methods for NoVs detection in food had significantly developed over the last 15 years. Currently NoVs were the most common pathogen accounting for 40% of outbreaks associated with fresh produce (i. e., fruits and vegetables). Data from outbreaks investigations verified fresh produce as the high risk food products for NoVs. The fresh produce were typically eaten raw with no thermal processing, can be contaminated at any step during production and processing from faecally polluted water and fertilizers, the poor hygiene practices by food handlers and the cross-contamination. The attachment of NoVs to the fresh produce was due to the physio-chemical factors of virus protein coat, the special attachment to different fresh produce, and the possibility for internalization of NoVs. It might provide answers to why those high risk foods were more frequently implicated (i. e., lettuce and raspberries). According to the data of foodborne NoVs outbreaks which were associated with fresh produce from EU countries and the USA, the outbreaks in EU countries were mainly associated with NoVs contaminated raspberries and lettuce, while in USA which were associated with NoVs contaminated lettuce. Unfortunately, there were no NoVs detection methods for fresh produce or the data of foodborne NoVs outbreaks which were associated with fresh produce in China. That made it difficult to analyze the NoVs contamination situation in China. The heterogeneous distributions of presumably low levels of virus, which presented in contaminated fresh produce, also made it difficult to detect NoVs. To solve this problem, different sampling methods, viral elution methods and RT-qPCR methods were chosen. For example, according to the isoelectric point of NoVs particles, high pH and high ionic strength solution could be used as means for releasing NoVs. For the elution from acidic fruit, the buffer capacity and the virus recovery could be increased by the addition of tris-HCl. When analyzing pectin containing raspberries or strawberries, the viral elution usually incubated with pectinase at neutral pH to avoid from foaming jelly. In this paper, the latest ISO standard for NoV detection in food and the new approaches for NoV detection were also reviewed to provide references for domestic researches. It was necessary to establish and develop domestic methods for NoV detection in fresh produce, especially the different NoV conventional molecular detection methods with corresponding NoV extraction methods, which targeted to the different adsorption characteristics of different fruits and vegetables, in order to strengthen the national food safety monitoring.
Food Analysis ; methods ; Food Contamination ; analysis ; Foodborne Diseases ; virology ; Fruit ; virology ; Gastroenteritis ; virology ; Humans ; Norovirus ; classification ; genetics ; isolation & purification ; physiology ; Vegetables ; virology

Objective To explore the effects of the multidisciplinary cooperative continue nursing on the quality of life of patients with chronic obstructive pulmonary disease (COPD). Methods From December 2012 to December 2014, hospitalized COPD patients who were in the stable stage of disease and would soon be discharged and met the criteria standards were chosen. Convenient extraction of 64 patients were set as the experimental group in one ward and 50 patients as the control group in the other ward. The conventional discharge guidance was employed in the control group. While the continuation of care before discharge and 1, 3, 6 months after discharge was employed in the experimental group. The indexes such as the quality of life and 6 min walking test distance (6MWD) were observed and compared. Results The total scores and symptoms, activities, influence scores of the quality of life were 48.53±15.78, 35.38± 18.61, 57.95 ±23.69, 52.28 ±15.27 after 6 months intervention in the experimental group and 60.24 ±10.14, 52.76 ±12.36, 68.34 ±15.59, 58.55 ±11.79 in the control group, and there were significant differences (t=-5.69--2.38, P<0.05). 6MWD was (267.46 ±64.64) m after 6 months intervention in the experimental group,which was more than that before intervention (169.42±48.46) m, and there was significant difference (t=-9.71, P<0.01). 6MWD was (201.32±53.93) m after 6 months intervention in the control group, which was not significantly better than that in the experimental group (t=5.82, P<0.01). Conclusions The quality of life of patients with COPD can be significantly improved by the multidisciplinary cooperative continue nursing education after discharge.

Objective:As a branch of image processing,image mining is a subject for great concern.With the development of image acquisition and storage,human can gain a lot of useful image information,but lack of effective analytic technique,so it is a focus of image mining that how to obtain useful image information and make full use of image information.So this paper gives an overview of the research and applications of image mining.Method:Firstly,it presents the concept and primary frame of image mining and the major techniques of lower layer image mining and higher layer image mining.Then,it discusses some applications in the biomedical,DNA analysis,data analysis of medical imaging and forensic medicine and so on.Result:Image mining is that the technique which can mine to discover new and valuable knowledge from vast collection of image.Conclusion:This article expounds the related technologies about image mining by our study of the image mining areas integrating the usual methods of the methods.Then,it identifies some applications in medicine and future research directions of image mining.

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